Deuterium magnetic resonance of selectively deuterated cholesteryl esters in phosphatidylcholine vesicles

Gorrissen, Heiner; Tulloch, A. P.; Cushley, Robert J.

doi:10.1021/bi00556a003

Cited by 34 publications

(22 citation statements)

References 33 publications

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“…This is in contrast to our previous observations on the egg phosphatidylcholine1CP-d3, system (6) in which 20 mol% cholesterol decreased the ester solubility at least by a factor of 2.…”

Section: Discussioncontrasting

confidence: 99%

“…The results indicate that dipalmitoylphosphatidylcholine vesicles can incorporate up to 5 mol% CP, an amount identical to that incorporated into egg phosphatidylcholine vesicles (6). The solubility of the ester is not greatly reduced in the gel phase as evidenced by the ZH nmr experiments conducted with a dipalmitoylphosphatidylcholine/CP-16,16,16-d3 vesicle system both above and 10°C below the gel to liquid crystalline phase transition of the phospholipid.…”

Section: Discussionmentioning

confidence: 64%

“…multilamellar liposomes prepared from the same phospholipid (28) and the same situation has also been encountered for the egg phosphatidylcholineICP system (5,6). The apparent increase in ester solubility upon going from liposomes to small vesicles may simply reflect sonication forcing an excess amount of cholesteryl ester into the bilayer, so that the final sample represents a supersaturated system.…”

Section: Discussionmentioning

confidence: 80%

“…The present study is an attempt to determine the bilayer systems was studied by epr of spin-labeled behavior of saturated cholesteryl ester in a highly CP in multilayers (2,3), as well as 13C nmr differ-curved bilayer composed of the completely satuence spectroscopy (4) and ZH nmr (using CP selec-rated phospholipid, dipalmitoylphosphatidyltively deuterated in the acyl chain) (5,6). chloline, both in the presence and absence of The epr studies indicated that only a small frac-cholesterol (20 mol%).…”

Section: Introductionmentioning

confidence: 99%

“…If vesicle radius may be estimated. Syntheses of hexadecanoic-2,2-d, and 3,3-d, acids were described previously (6).…”

Section: Introductionmentioning

confidence: 99%

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Deuterium and phosphorus-31 nuclear magnetic resonance of cholesteryl palmitate in dipalmitoylphosphatidylcholine model membranes

Cushley¹,

Gorrissen²,

Wassall³

1980

Can. J. Chem.

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Dedicated to Prof. Raymond U. Lemieux on the occasion of his 60th birthday ROBERT J. CUSHLEY, HEINERGORRISSEN, and STEPHEN R. WASSALL. Can. J. Chem. 58,2433Chem. 58, (1980. Cholesteryl palmitate (CP) has been incorporated into vesicles of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylcholine/20 mol% cholesterol, in amounts up to 5 mol%. By means of 3'P lanthanide induced shifts the average diameter of these vesicles was calculated to be -270 A.The 2H spectral linewidths of selectively deuterated CP incorporated into liquid crystalline dipalmitoylphosphatidylcholine vesicles are S 150 Hz, implying a low degree of order for the ester acyl chain. Addition of 20 mol% cholesterol to the vesicles increases the linewidths which, nevertheless, remain 5 185 Hz. The corresponding 2H longitudinal relaxation times (1450 ms) indicate that the fast segmental motions of the ester chain are slower than for the phospholipid chains.ROBERT J. CUSHLEY, HEINER GORRISSEN et STEPHEN R. WASSALL. Can. J. Chem. 58,2433Chem. 58, (1980. On a incorpore le palmitate de cholesteryle (PC) en quantite allant jusqu'i 5 mol% dans les vesicules de dipalmitoylphosphatidylcholine et de dipalmitoylphosphatidylcholine/20 mol% cholesterol. Au moyen des deplacements chimiques du )'P induits par les lanthanides on a calculk que le diambtre moyen de ces vesicules est d'environ 270 A. Les largeurs des raies des spectres rmn 2H des PC deuteres selectivement et incorpores dans les vCsicules de dipalmitoylphosphatidylcholine cristallins B I'etat liquide sont S 150 Hz; ceci implique un faible degre d'ordre de la chaine ester acyc1ique.L'addition de 20 mol% de cholest6rol aux vesicules augmente la largeur des raies qui nkanmoins restent i 185 Hz. Les temps de relaxation longitudinale correspondants du 2H (2450 ms) indiquent que les rapides mouvements des segments de la chaine ester sont plus lents que ceux des chaines phospholipides.[Traduit par le journal] Introduction the phospholipid is changed from phosphatidylWe have been studying the structure and proper-choline to sphingomyelin the amount of interties of cholesteryl esters in various phospholipid calated CP reaches 1.5 mol% (7). membrane systems using a diversity of magnetic When eggphosphatidylcholine bilayers are made resonance techniques. In our initial study (1) we by sonication of aqueous dispersions to form highly utilized 31P nmr spectroscopy and found that curved unilamellar vesicles, ester intercalation incholesteryl palmitate (CP) increased the permea-creases substantially, reaching as high as 5 mol% bility of model membranes to ions, whereas (6) while the ester chains appear more disordered cholesteryl linoleate did not. The behavior of the compared to CP in multilamellar liposomes (5). saturated ester CP in egg phosphatidylcholineThe present study is an attempt to determine the bilayer systems was studied by epr of spin-labeled behavior of saturated cholesteryl ester in a highly CP in multilayers (2, 3), as well as 13C nmr differ-curved bilayer composed of the completely satuenc...

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“…This is in contrast to our previous observations on the egg phosphatidylcholine1CP-d3, system (6) in which 20 mol% cholesterol decreased the ester solubility at least by a factor of 2.…”

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

“…If vesicle radius may be estimated. Syntheses of hexadecanoic-2,2-d, and 3,3-d, acids were described previously (6).…”

Section: Introductionmentioning

confidence: 99%

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Deuterium and phosphorus-31 nuclear magnetic resonance of cholesteryl palmitate in dipalmitoylphosphatidylcholine model membranes

Cushley¹,

Gorrissen²,

Wassall³

1980

Can. J. Chem.

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The Lipid Droplet and the Endoplasmic Reticulum

Ohsaki

Sołtysik

Fujimoto

2017

Advances in Experimental Medicine and Biology

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Lipid droplets (LDs) are often found adjacent to the endoplasmic reticulum (ER). The ER-LD association may appear morphologically similar to the prototypical membrane contact sites found between the ER and other organelles, but the functional relationship between the ER and LDs is unique in that highly hydrophobic lipid esters are transported between them. This transportation is thought to occur through some form of membrane continuity, but its details are yet to be defined. Lipin, seipin, and FIT proteins, which are located at the ER-LD interface, may be involved in the lipid ester transport and probably play important roles for functional connectivity of the two organelles. More recently, LDs in the nucleus were found to be closely adhered to the inner nuclear membrane, representing a specialized form of the ER-LD association. In this article, we will give an overview of the ER-LD association, which is still filled with many unanswered questions.

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Deuterium magnetic resonance of selectively deuterated cholesteryl esters in dipalmitoyl phosphatidylcholine dispersions

Gorrissen

MacKay

Wassall

et al. 1981

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Deuterium magnetic resonance of selectively deuterated cholesteryl esters in phosphatidylcholine vesicles

Cited by 34 publications

References 33 publications

Deuterium and phosphorus-31 nuclear magnetic resonance of cholesteryl palmitate in dipalmitoylphosphatidylcholine model membranes

Deuterium and phosphorus-31 nuclear magnetic resonance of cholesteryl palmitate in dipalmitoylphosphatidylcholine model membranes

The Lipid Droplet and the Endoplasmic Reticulum

Deuterium magnetic resonance of selectively deuterated cholesteryl esters in dipalmitoyl phosphatidylcholine dispersions

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