Developed fungal–bacterial biofilms as a novel tool for bioremoval of hexavelant chromium from wastewater

Herath, Lasantha; Rajapaksha, Anushka Upamali; Vithanage, Meththika; Seneviratne, G.

doi:10.1080/02757540.2013.861828

Cited by 28 publications

(10 citation statements)

References 32 publications

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“…Two kinds of BC were used: BC300 was produced from Gliricidia sepium (Fabaceae) biomass pyrolysed at a constant temperature of 300°C for 3 h using a muffle furnace (P300, Nabertherm, Germany), while BC700 was obtained directly from the bioenergy industry in Sri Lanka. Metal resistance bacteria were isolated from Wasgamuwa serpentine soil and combined with a garden soil fungus (Aspergillus niger) in the FB medium (Herath et al 2014;Seneviratne et al 2015a).…”

Section: Soil Amendments and Treatmentsmentioning

confidence: 99%

“…Studies conducted to date suggest that heavy metal immobilization is greater under co-inoculation than when using mono or mixed cultures of bacterial inoculations (Herath et al 2014;Seneviratne et al 2015b). Therefore, there is a potential for co-inoculation to be used in wastewater reactors for heavy metal remediation (Bestawy et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Role of woody biochar and fungal-bacterial co-inoculation on enzyme activity and metal immobilization in serpentine soil

et al. 2015

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Purpose In this study, we investigated the effect of biochar (BC) and fungal bacterial co-inoculation (FB) on soil enzymatic activity and immobilization of heavy metals in serpentine soil in Sri Lanka. Materials and methods A pot experiment was conducted with tomatoes (Lycopersicon esculentum L.) at 1, 2.5, and 5 % (w/ w) BC ratios. Polyphenol oxidase, catalase and dehydrogenase activities were determined by idometric, potassium permanganate oxidisable, and spectrophotometric methods, respectively. Heavy metal concentrations were assessed by 0.01 M CaCl 2 and sequential extraction methods. Results and discussion An increase in BC application reduced polyphenol oxidase, dehydrogenase, and catalase activity. The application of FB increased soil dehydrogenase activity, with the maximum activity found in 1 % BC700+FB treatment. Moreover, the CaCl 2 extractable metals (Ni, Mn, and Cr) in 5 % BC700 amended soil decreased by 92, 94, and 100 %, respectively, compared to the control. Sequential extraction showed that the exchangeable concentrations of Ni, Mn, and Cr decreased by 55, 70, and 80 % in 5 % BC700, respectively. Conclusions Results suggest that the addition of BC to serpentine soil immobilizes heavy metals and decreases soil enzymatic activities. The addition of FB to serpentine soil improves plant growth by mitigating heavy metal toxicity and enhancing soil enzymatic activities.

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Section: Soil Amendments and Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of woody biochar and fungal-bacterial co-inoculation on enzyme activity and metal immobilization in serpentine soil

et al. 2015

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“…Additionally, microorganisms may be involved in the formation of multispecies biofilms, providing stability for formed communities [ 85 ]. Numerous recent studies [ 84 , 86 , 87 ] have shown a promising trend in the applications of the fungal–bacterial biofilm in diverse fields.…”

Section: Discussionmentioning

confidence: 99%

Revealing of Non-Cultivable Bacteria Associated with the Mycelium of Fungi in the Kerosene-Degrading Community Isolated from the Contaminated Jet Fuel

Шапиро

Chekanov

Alexandrova

et al. 2021

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Fuel (especially kerosene) biodamage is a challenge for global industry. In aviation, where kerosene is a widely used type of fuel, its biodeterioration leads to significant damage. Six isolates of micromycetes from the TS-1 aviation kerosene samples were obtained. Their ability to grow on the fuel was studied, and the difference between biodegradation ability was shown. Micromycetes belonged to the Talaromyces, Penicillium, and Aspergillus genera. It was impossible to obtain bacterial isolates associated with their mycelium. However, 16S rRNA metabarcoding and microscopic observations revealed the presence of bacteria in the micromycete isolates. It seems to be that kerosene-degrading fungi were associated with uncultured bacteria. Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes were abundant in the fungal cultures isolated from the TS-1 jet fuel samples. Most genera among these phyla are known as hydrocarbon degraders. Only bacteria-containing micromycete isolates were able to grow on the kerosene. Most likely, kerosene degradation mechanisms are based on synergism of bacteria and fungi.

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“…Furthermore, one of the most recurring features of biofilm-associated cells is a high tolerance to antimicrobial agents, such as antibiotics, disinfectants, and biocides, which makes biofilms a major problem in clinical and industrial settings (Finkel and Mitchell, 2011; Wolfmeier et al, 2017). These properties have beneficial aspects in bioremediation, where biofilms were often more efficient than planktonic counterparts (Singh et al, 2006; Seneviratne et al, 2008; Pesciaroli et al, 2013a,c; Herath et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Time-Dependent Changes in Morphostructural Properties and Relative Abundances of Contributors in Pleurotus ostreatus/Pseudomonas alcaliphila Mixed Biofilms

et al. 2019

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Pleurotus ostreatus dual biofilms with bacteria are known to be involved in rock phosphate solubilization, endophytic colonization, and even in nitrogen fixation. Despite these relevant implications, no information is currently available on the architecture of P. ostreatus -based dual biofilms. In addition to this, there is a limited amount of information regarding the estimation of the temporal changes in the relative abundances of the partners in such binary systems. To address these issues, a dual biofilm model system with this fungus was prepared by using Pseudomonas alcaliphila 34 as the bacterial partner due to its very fast biofilm-forming ability. The application of the bacterial inoculum to already settled fungal biofilm on a polystyrene surface coated with hydroxyapatite was the most efficient approach to the production of the mixed system the ultrastructure of which was investigated by a multi-microscopy approach. Transmission electron microscopy analysis showed that the adhesion of bacterial cells onto the mycelial cell wall appeared to be mediated by the presence of an abundant layer of extracellular matrix (ECM). Scanning electron microscopy analysis showed that ECM filaments of bacterial origin formed initially a reticular structure that assumed a tabular semblance after 72 h, thus overshadowing the underlying mycelial network. Across the thickness of the mixed biofilms, the presence of an extensive network of channels with large aggregates of viable bacteria located on the edges of their lumina was found by confocal laser scanning microscopy; on the outermost biofilm layer, a significant fraction of dead bacterial cells was evident. Albeit with tangible differences, similar results regarding the estimation of the temporal shifts in the relative abundances of the two partners were obtained by two independent methods, the former relying on qPCR targeting of 16S and 18S rRNA genes and the latter on ester-linked fatty acid methyl esters analysis.

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Developed fungal–bacterial biofilms as a novel tool for bioremoval of hexavelant chromium from wastewater

Cited by 28 publications

References 32 publications

Role of woody biochar and fungal-bacterial co-inoculation on enzyme activity and metal immobilization in serpentine soil

Role of woody biochar and fungal-bacterial co-inoculation on enzyme activity and metal immobilization in serpentine soil

Revealing of Non-Cultivable Bacteria Associated with the Mycelium of Fungi in the Kerosene-Degrading Community Isolated from the Contaminated Jet Fuel

Time-Dependent Changes in Morphostructural Properties and Relative Abundances of Contributors in Pleurotus ostreatus/Pseudomonas alcaliphila Mixed Biofilms

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