2021
DOI: 10.1002/nafm.10708
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Developing a Standardized Single Nucleotide Polymorphism Panel for Rangewide Genetic Monitoring of Bull Trout

Abstract: Conservation of threatened and endangered species often involves large-scale collaboration across jurisdictional boundaries, but such efforts are typically hampered by a lack of consistent monitoring data at local, regional, and species-distributional scales. Contemporary methods of genetic monitoring that are grounded in sequencing-based approaches offer a powerful means to achieve the data consistency necessary for conservation of high-profile species. Here, we describe the collaborative, multiagency develop… Show more

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Cited by 8 publications
(8 citation statements)
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“…With this possibility, managers would be able to combine phenotypic information and rapid genomic testing for barrier management across the landscape as is done for rapid Bull Trout population assignments (Bohling et al. 2021).…”
Section: Discussionmentioning
confidence: 99%
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“…With this possibility, managers would be able to combine phenotypic information and rapid genomic testing for barrier management across the landscape as is done for rapid Bull Trout population assignments (Bohling et al. 2021).…”
Section: Discussionmentioning
confidence: 99%
“…Advancements in genomic techniques will likely allow for the detection of hybridization at enough markers for reliable individual admixture estimation with a faster turnaround capacity, making this a more practical consideration for future applications. With this possibility, managers would be able to combine phenotypic information and rapid genomic testing for barrier management across the landscape as is done for rapid Bull Trout population assignments (Bohling et al 2021).…”
Section: Discussionmentioning
confidence: 99%
“…Further publications of GTSeq genotype error rates and the establishment of a reference sample database may help to increase consistency among laboratories. Similar approaches have been successful for microsatellite panels (Seeb et al, 2007 ; Stott et al, 2010 ) and have begun to be used for GTSeq panels (Bohling et al, 2021 ; Hayward et al, 2022 ). However, the appropriate use of positive and negative controls should help account for batch effects in future studies.…”
Section: Discussionmentioning
confidence: 99%
“…We were unable to identify the reason for the lower sequencing quality obtained from the OMNRF laboratory; however, we predict that it is likely associated with slight differences TA B L E 3 Among-laboratory genotype congruence statistics for individuals with a genotype rate greater than 50% for all types of markers (All), microhaplotypes (mhaps), and single nucleotide polymorphisms (SNPs). (Seeb et al, 2007;Stott et al, 2010) and have begun to be used for GTSeq panels (Bohling et al, 2021;Hayward et al, 2022). However, the appropriate use of…”
Section: Interjurisdictional Collaborationmentioning
confidence: 99%
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