Developing an ultra non-fouling SU-8 and PDMS hybrid microfluidic device by poly(amidoamine) engraftment

Qin, Yubo; Yeh, Po-Ying; Hao, Xingkai; Cao, Xudong

doi:10.1016/j.colsurfb.2015.01.042

Cited by 32 publications

(16 citation statements)

References 46 publications

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“…All procedures were based on well‐established protocols reported elsewhere (Qin, Yeh, Hao, & Cao, ; Yeh, Zhang, Lin, & Cao, ). A photograph of the resulting microfluidic device and its inner surface modification procedures are shown in Figure S1a–d.…”

Section: Methodsmentioning

confidence: 99%

“…1-(3-dimethylaminopropyl)-3-ethylcarbodiimide was obtained from Alfa Aesar (Ward Hill, MA). 2.2 | Fabrication and surface modification of PDMS microfluidic device with E. coli O157:H7 capturing aptamers All procedures were based on well-established protocols reported elsewhere (Qin, Yeh, Hao, & Cao, 2015;Yeh, Zhang, Lin, & Cao, 2012). A photograph of the resulting microfluidic device and its inner surface modification procedures are shown in Figure S1a…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, highly branched dendrimers were immobilized on the inner surfaces of the PDMS microchannels and the capturing aptamers were consequently conjugated to the dendrimer coated PDMS surfaces via functional groups of the PAMAM dendrimers. The benefits of using the PAMAM dendrimers were twofold as we have demonstrated in our previous work (Qin et al, 2015;Qin, Yang, Zhang, & Cao, 2017): (1) the dendrimers are shown to render modified surfaces nonfouling to minimize nonspecific bindings, thus reducing background signals, and (2) the immobilized multifunctional dendrimers can provide multiple binding sites for subsequent conjugating multiple copies of E. coli O157:H7 capturing aptamers to significantly increase capturing surface areas and probabilities. Buffer solutions spiked with E. coli O157:H7 cells at different concentrations (i.e., 10 2 , 10 3 , 10 4 , and 10 5 cells/mL) were injected into the dendrimer-aptamer modified PDMS devices at a flow rate of 0.05 mL/hr for 1 hr.…”

Section: Dendrimer-aptamer-rca Detection Systemmentioning

confidence: 98%

“…PDMS microfluidic channels were successfully prepared and their inner surfaces were modified with E. coli O157:H7 capturing aptamers as reported in our previous studies(Qin et al, 2015;Yeh et al, 2012).…”

mentioning

confidence: 99%

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Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Jiang

Qiu

et al. 2019

Journal of Food Safety

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Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained from the AFM study are combined with those from the conventional electrophoresis method to optimize RCA reactions for rapid and sensitive detection. We show that there appears to be an inhomogeneous RCA initiation phase in early to mid‐stage of the RCA reaction where some chains grow faster while others grow slower or remain dormant, an observation that has not been reported in the literature. Furthermore, we demonstrate that the RCA can significantly enhance detection signals by up to 100‐fold. We also show that the Escherichia coli O157:H7 detection with the RCA can be carried out in different food matrices with excellent detection sensitivities and specificities. In conclusion, these results suggest that a microfluidic device in combination with RCA signal enhancement is a simple and robust approach to sensitive whole‐cell detection in food samples. Practical applications The current research has exciting potentials for applications in sensitive detections of food samples for food safety inspections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dendrimer-aptamer-rca Detection Systemmentioning

confidence: 98%

mentioning

confidence: 99%

See 2 more Smart Citations

Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Jiang

Qiu

et al. 2019

Journal of Food Safety

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“…The photoresist SU-8 has several attractive properties like good thermal and chemical stability and the possibility to create a broad range of layer thicknesses by spin coating and outstanding high aspect ratios. SU-8 can be used as optical waveguide; 1 as encapsulation or insulation material; 2 and as bulk material for the fabrication of lab-on-a-chip systems, microreactors, or sensors. 1,[3][4][5] Microsystems offer, due to their small size, the possibility of handling and analysis of small fluid volumes; hence, their application for biological and medical analysis is attractive.…”

Section: Introductionmentioning

confidence: 99%

Development of a process for the manufacturing of SU-8 100 for the use in cell culture

Blume

Mielke

Kohnert

et al. 2018

Journal of Bioactive and Compatible Polymers

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SU-8 100 is a negative-tone photoresist which is used for the manufacturing of structures with high thickness and aspect ratio. The use of SU-8 100 systems in cell culture is difficult due to its cytotoxicity resulting from leachables. In this article, the production process of non-cytotoxic SU-8 100 foils is described. The aim is to create a polymer scaffold with integrated sensors in order to enable online measurements of cell growth and viability within a three-dimensional cell culture. This scaffold could be a useful tool for testing biological effects of pharmaceutical substances. Therefore, the scaffold material must not have a negative influence on the cell growth and viability. Among the biocompatibility tests described in DIN EN ISO 10993-5, the neutral red uptake method was chosen to evaluate the cytotoxicity of polymer foils. The production process of SU-8 100 was altered in such a way that non-cytotoxic SU-8 100 foils can be manufactured.

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Breaking The Permeance‐Selectivity Tradeoff for Post‐Combustion Carbon Capture: A Bio‐Inspired Strategy to Form Ultrathin Hollow Fiber Membranes

Hillman,

Wang,

Liang

et al. 2023

Advanced Materials

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Thin film composite (TFC) hollow fiber membranes with ultrathin selective layer are desirable to maximize the gas permeance for practical applications. Herein we propose a bio‐inspired strategy to fabricate sub‐100‐nm membranes via a tree‐mimicking polymer network, term as PDMS‐PEG, with amphipathic components featuring multi‐functionalities. The hydrophobic polydimethylsiloxane (PDMS) brushes act as the roots that can strongly cling to the gutter layer, the PDMS crosslinkers function as the xylems to enable fast gas transport, and the hydrophilic ethylene‐oxide moieties (brushes and mobile molecules) resemble tree leaves that selectively attract CO2 molecules. As a result, a ∼ 27 nm‐thick PDMS‐PEG selective layer can be attached onto the hollow fiber supported PDMS gutter layer through simple dip‐coating method without any modification. Furthermore, a CO2 permeance of ∼ 2700 GPU and a CO2/N2 selectivity of ∼ 21 that is beyond the permeance‐selectivity upper bound for hollow fiber membranes is achieved. Our bio‐inspired concept can potentially open the possibility of scalable hollow fiber membranes production for commercial applications in post‐combustion carbon capture and beyond.This article is protected by copyright. All rights reserved

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Developing an ultra non-fouling SU-8 and PDMS hybrid microfluidic device by poly(amidoamine) engraftment

Cited by 32 publications

References 46 publications

Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Development of a process for the manufacturing of SU-8 100 for the use in cell culture

Breaking The Permeance‐Selectivity Tradeoff for Post‐Combustion Carbon Capture: A Bio‐Inspired Strategy to Form Ultrathin Hollow Fiber Membranes

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