Developing chick embryos express a protein which shares homology with the nuclear pore complex protein Nup88 present in human tumors

Abstract

Nup88 is a nuclear pore complex protein which is overexpressed in a variety of human tumors of the stomach, colon, liver, pancreas, breast, lung, ovary, uterus, prostate and kidney. A monoclonal antibody crossreacting with the yeast Candida albicans and Nup88 was used to investigate the expression of cross-reactive antigens in chick embryos, in an attempt to identify an experimental model for studying the role played by Nup88 during cell development and differentiation. All cells in the trilaminar embryo were labeled with the antibody, but as development advanced and organogenesis was completed, expression of the corresponding antigen became more restricted. Thus, some structures continued to be intensely labeled (skin epithelium, oropharyngeal endothelium, perichondral mesenchymal tissue), whereas others ( muscular tissue, vascular endothelium, respiratory endothelium, digestive tract mucosa, peripheral nerves, medullary white matter and the retinal axons) were more moderately stained. No immunoreactivity was observed in the medullary grey matter or cartilage. A specific band of 53 kDa observed by Western blotting of chick embryo extracts suggested that the chicken antigen recognized by the monoclonal antibody is the homologue of human Nup88, which is associated with the high proliferation and low differentiation of tumor cells. The present results indicate that the role of Nup88 in cell differentiation and organ development could be fruitfully investigated using the developing chick embryo as an experimental model.

Antifungal and Antitumor Activities of a Monoclonal Antibody Directed Against a Stress Mannoprotein of Candida albicans

“…The similarities shown by Mabs C7 and C6 suggested that Mab C7 might also react with Nup88. This possibility has been recently confirmed using a 27 kDa recombinant fragment from Nup88 obtained in Escherichia coli [58].…”

“…The diagnostic usefulness of Mab C7 was studied by means of immunohistochemistry in a series of ovarian cancers, showing a granular staining with marked perinuclear accumulation (Figure 11). However, Mab C7 showed a more diffuse cytoplasmic pattern in oral squamous cell carcinomas [58].…”

Nup88 Expression in Normal Mucosa, Adenoma, Primary Adenocarcinoma and Lymph Node Metastasis in the Colorectum

“…The polyclonal rabbit antiserum against Nup88 was the same employed in previous studies by our group [5][6][7][8][10][11][12] . The preparation, specificity and reliability of the rabbit polyclonal Nup88 antibody used in this study were as described previously [5][6][7][8][10][11][12] .…”

“…The preparation, specificity and reliability of the rabbit polyclonal Nup88 antibody used in this study were as described previously [5][6][7][8][10][11][12] .…”

Nup88 Expression is Associated With Myometrial Invasion in Endometrial Carcinoma

“…8 Subsequently, we studied Nup88-mRNA expression in breast cancer and showed it to be associated with all clinical and biological features defining a significantly more aggressive tumor phenotype. 9 From these results, we concluded that Nup88 could serve as a new biological marker of aggressiveness in breast cancer.…”