Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds

Xu, Ronald X.; Allen, David W.; Huang, Jiwei; Gnyawali, Surya; Melvin, J. Stuart; Elgharably, Haytham; Huang, Kun; Bergdall, Valerie; Litorja, Maritoni; Rice, Joseph P.; Hwang, Jeeseong; Sen, Chandan K.

doi:10.1364/boe.3.001433

Cited by 34 publications

(27 citation statements)

References 17 publications

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“…7 TegadermÔ, an oxygen permeable adhesive dressing, was used to seal a reservoir beneath the TCOM probe. The reservoir contained buffered saline solution for measurement of dissolved oxygen.…”

Section: Study Visitsmentioning

confidence: 99%

Standardized Approach to Quantitatively Measure Residual Limb Skin Health in Individuals with Lower Limb Amputation

Rink

Wernke

Powell

et al. 2017

Advances in Wound Care

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Objective: (1) Develop a standardized approach to quantitatively measure residual limb skin health. (2) Report reference residual limb skin health values in people with transtibial and transfemoral amputation. Approach: Residual limb health outcomes in individuals with transtibial (n = 5) and transfemoral (n = 5) amputation were compared to able-limb controls (n = 4) using noninvasive imaging (hyperspectral imaging and laser speckle flowmetry) and probe-based approaches (laser doppler flowmetry, transcutaneous oxygen, transepidermal water loss, surface electrical capacitance). Results: A standardized methodology that employs noninvasive imaging and probebased approaches to measure residual limb skin health are described. Compared to able-limb controls, individuals with transtibial and transfemoral amputation have significantly lower transcutaneous oxygen tension, higher transepidermal water loss, and higher surface electrical capacitance in the residual limb. Innovation: Residual limb health as a critical component of prosthesis rehabilitation for individuals with lower limb amputation is understudied in part due to a lack of clinical measures. Here, we present a standardized approach to measure residual limb health in people with transtibial and transfemoral amputation. Conclusion: Technology advances in noninvasive imaging and probe-based measures are leveraged to develop a standardized approach to quantitatively measure residual limb health in individuals with lower limb loss. Compared to able-limb controls, resting residual limb physiology in people that have had transfemoral or transtibial amputation is characterized by lower transcutaneous oxygen tension and poorer skin barrier function.

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Section: Study Visitsmentioning

confidence: 99%

Standardized Approach to Quantitatively Measure Residual Limb Skin Health in Individuals with Lower Limb Amputation

Rink

Wernke

Powell

et al. 2017

Advances in Wound Care

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“…For example, a homogeneous liquid phantom incorporating porcine blood at a range of Hb saturation levels was implemented for quantitative evaluation of an HRI system. 12 Phantoms with submillimeter channel geometry and capability for varying saturation levels have been fabricated using components such as straight, thinwalled glass capillary tubes embedded in silicone, resin, or polyurethane. 1,2 However, phantoms incorporating biomimetic vascular geometries may enable a more thorough understanding of the effect of tissue morphology on oximetry measurement accuracy, as well as other tissue and device parameters (e.g., wavelength) that influence device performance.…”

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confidence: 99%

Rapid prototyping of biomimetic vascular phantoms for hyperspectral reflectance imaging

et al. 2015

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Abstract. The emerging technique of rapid prototyping with three-dimensional (3-D) printers provides a simple yet revolutionary method for fabricating objects with arbitrary geometry. The use of 3-D printing for generating morphologically biomimetic tissue phantoms based on medical images represents a potentially major advance over existing phantom approaches. Toward the goal of image-defined phantoms, we converted a segmented fundus image of the human retina into a matrix format and edited it to achieve a geometry suitable for printing. Phantoms with vessel-simulating channels were then printed using a photoreactive resin providing biologically relevant turbidity, as determined by spectrophotometry. The morphology of printed vessels was validated by xray microcomputed tomography. Channels were filled with hemoglobin (Hb) solutions undergoing desaturation, and phantoms were imaged with a near-infrared hyperspectral reflectance imaging system. Additionally, a phantom was printed incorporating two disjoint vascular networks at different depths, each filled with Hb solutions at different saturation levels. Light propagation effects noted during these measurements-including the influence of vessel density and depth on Hb concentration and saturation estimates, and the effect of wavelength on vessel visualization depth-were evaluated. Overall, our findings indicated that 3-D-printed biomimetic phantoms hold significant potential as realistic and practical tools for elucidating light-tissue interactions and characterizing biophotonic system performance. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Further research is necessary to optimize the material system and the engineering design of the phantom FDM technique for freeform fabrication of 3-D tissue-simulating phantoms with high productivity and fidelity. In the future, the proposed phantom FDM technique can potentially be used in combination with the digital tissue phantoms 25 to establish a traceable standard for calibration and performance validation of many biomedical optical devices. …”

Section: Resultsmentioning

confidence: 99%

Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging

et al. 2015

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Abstract. Biomedical optical devices are widely used for clinical detection of various tissue anomalies. However, optical measurements have limited accuracy and traceability, partially owing to the lack of effective calibration methods that simulate the actual tissue conditions. To facilitate standardized calibration and performance evaluation of medical optical devices, we develop a three-dimensional fuse deposition modeling (FDM) technique for freeform fabrication of tissue-simulating phantoms. The FDM system uses transparent gel wax as the base material, titanium dioxide (TiO 2 ) powder as the scattering ingredient, and graphite powder as the absorption ingredient. The ingredients are preheated, mixed, and deposited at the designated ratios layerby-layer to simulate tissue structural and optical heterogeneities. By printing the sections of human brain model based on magnetic resonance images, we demonstrate the capability for simulating tissue structural heterogeneities. By measuring optical properties of multilayered phantoms and comparing with numerical simulation, we demonstrate the feasibility for simulating tissue optical properties. By creating a rat head phantom with embedded vasculature, we demonstrate the potential for mimicking physiologic processes of a living system. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds

Cited by 34 publications

References 17 publications

Standardized Approach to Quantitatively Measure Residual Limb Skin Health in Individuals with Lower Limb Amputation

Standardized Approach to Quantitatively Measure Residual Limb Skin Health in Individuals with Lower Limb Amputation

Rapid prototyping of biomimetic vascular phantoms for hyperspectral reflectance imaging

Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging

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