Developing of regenerated plants of winter bread wheat using the anther culture method

Abstract

Abstract. The purpose of the current study was to evaluate the effect of cultivation conditions and the genotype of winter bread wheat samples on the formation of embryogenic callus and regenerative capacity, to identify regenerated plants in order to develop new initial material for breeding new varieties. Scientific novelty. There has been estimated the share of influence of induction nutrient media with different composition and genotype on the amount of regenerated plants of winter wheat in anther culture; there have been identified the new samples with high regeneration potential. Methods. The objects of the study were the donor plants of winter bread wheat (Triticum aestivum L.) of F3 generation from breeding nurseries of the winter wheat department of the FSBSI “ARC “Donskoy”, namely 13 hybrid combinations developed in the laboratory for breeding and seed production of winter bread wheat of half-intensive type. There has been used a methodology of obtaining haploids in anther culture in vitro including such methods as sterilization during work with in vitro; preparation of nutrient media; selection and pretreatment of plant explants; determination of the stage of microspores’ development; isolation and planting of anthers on an induction nutrient medium; regeneration of plants from callus tissues. Results. There has been found the most favorable nutrient media for the induction of and rogenesis in vitro. There has been identified a maximum percentage of new formations from the anthers of winter bread wheat in the sample Vol’nitsa × Gerda. There have been developed the largest number of green haploid regenerated plants from pollen callus from the samples Vol’nitsa × Gerda, Kapitan × Vol’nyy Don. There has been revealed that the contribution of the genotype and nutrient medium into the number of new formations and the regeneration of green plants was statistically significant.