Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system

Abstract

To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA) n film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N- diazeniumdiolate (NONOates), into the (Gel/TA) n film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA) n film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus .

A Hydrogen‐Bonded Extracellular Matrix‐Mimicking Bactericidal Hydrogel with Radical Scavenging and Hemostatic Function for pH‐Responsive Wound Healing Acceleration

“…[13] Tyrosine and serine amino acids with free hydroxyl groups are present in the structure of PSG that can create abundant physical (noncovalent) hydrogen bonding with TA to increase the strength of the final GelTA hydrogel. [30,37] Proline and 4-hydroxyproline sites are also present in the structure of gelatin for hydrophobic interactions with pentagallyol glucose of TA to further promote the stabilization of the hydrogel after the formation, [38][39][40] resulting in the fabrication of GelTA by a simple approach without adding any chemical crosslinker. In addition, gelatin has very low cost, low immunogenicity, and high biocompatibility, which are very important criteria for a raw material when the aim is the design of a tissue regenerative product with bench to bedside movement potential.…”

Polymeric Nitric Oxide Delivery Nanoplatforms for Treating Cancer, Cardiovascular Diseases, and Infection

“…The film with a roughness, porous structure enabled burst release of NO gas that can have bactericidal effects. [ 71 ]…”

“…The film with a roughness, porous structure enabled burst release of NO gas that can have bactericidal effects. [71] Although the postmodification method provided a feasible way to prepare polymeric NO donors, it remained challenging to synthesize NO-releasing polymers through the direct polymerization approach. Notably, the postmodification method generally led to incomplete chemical modification of functional groups, due to the steric hindrance effect.…”

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

“…The incorporation of bioactive molecules into multilayers is a broadly studied topic and a well-established means to prevent this loss of antimicrobial activity, with a variety of biomolecules including proteins and peptides 5 , or particles 6 , 7 and other small molecules 8 able to be embedded into multilayers. Such films are already investigated for a variety of applications including for medical research as a means to accelerate wound healing 9 or to lower infection rates due to biofilm growth on biomedical stents and other implanted devices 10 .…”