Developing the cryomacroscope for cryopreservation applications

Feig, Justin S.G.; Williams, Alexander C.; Lin, Christopher C.; Rabin, Yoed

doi:10.1109/nebc.2012.6207019

Cited by 2 publications

(2 citation statements)

References 4 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While thermal expansion drives thermo-mechanical stress, potentially leading to fracture, in combination with surface tension it may also deform the free surface of the glass-forming material [13]. This deformed surface—the surface through which visualization is achieved—may result in a “magnifying glass” effect in larger samples, where the accumulated effect of contraction becomes significant.…”

Section: Resultsmentioning

confidence: 99%

A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators

et al. 2013

Self Cite

View full text Add to dashboard Cite

The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events in cryoprotective cocktails containing synthetic ice modulators (SIM)—compounds which hinder ice crystal growth. Cryopreservation by vitrification is a highly complex application, where the likelihood of crystallization, fracture formation, degradation of the biomaterial quality, and other physical events are dependent not only upon the instantaneous cryogenic conditions, but more significantly upon the evolution of conditions along the cryogenic protocol. Nevertheless, cryopreservation success is most frequently assessed by evaluating the cryopreserved product at its end states—either at the cryogenic storage temperature or room temperature. The cryomacroscope is the only available device for visualization of large-size specimens along the thermal protocol, in an effort to correlate the quality of the cryopreserved product with physical events. Compared with earlier cryomacroscope prototypes, the new Cryomacroscope-III evaluated here benefits from a higher resolution color camera, improved illumination, digital recording capabilities, and high repeatability in tested thermal conditions via a commercial controlled-rate cooler. A specialized software package was developed in the current study, having two modes of operation: (a) experimentation mode to control the operation of the camera, record camera frames sequentially, log thermal data from sensors, and save case-specific information; and (b) post-processing mode to generate a compact file integrating images, elapsed time, and thermal data for each experiment. The benefits of the Cryomacroscope-III are demonstrated using various tested mixtures of SIMs with the cryoprotective cocktail DP6, which were found effective in preventing ice growth, even at significantly subcritical cooling rates with reference to the pure DP6.

show abstract

Section: Resultsmentioning

confidence: 99%

A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fracture formation is often an indication of structural failure of larger specimens such as heart valves [1], and blood vessels [2,13]. A new device has been developed recently—the cryomacroscope—to investigate fracturing events during the processes of cryopreservation [4,15,20]. Unfortunately, the correlation of structural damage with the development of thermo-mechanical stress in cryopreserved specimens represents a largely unexplored area in cryopreservation research.…”

Section: Introductionmentioning

confidence: 99%

Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators

et al. 2014

Self Cite

View full text Add to dashboard Cite

This study provides thermal expansion data for blood vessels permeated with the cryoprotective cocktail DP6, when combined with selected synthetic ice modulators (SIMs): 12% polyethylene glycol 400, 6% 1,3-cyclohexanediol, and 6% 2,3-butanediol. The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. The current study is part of an ongoing effort to characterize thermo-mechanical effects on structural integrity of cryopreserved materials, where thermal expansion is the driving mechanism to thermo-mechanical stress. This study focuses on the lower part of the cryogenic temperature range, where the cryoprotective agent (CPA) behaves as a solid for all practical applications. By combining results obtained in the current study with literature data on the thermal expansion in the upper part of the cryogenic temperature range, unified thermal expansion curves are presented.

show abstract

Developing the cryomacroscope for cryopreservation applications

Cited by 2 publications

References 4 publications

A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators

A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators

Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators

Contact Info

Product

Resources

About