Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies

Wan, Ying; Shi, Zhengwang; Peng, Gaochaung; Wang, Lijuan; Luo, Juncong; Ru, Yi; Zhou, Gaijing; Ma, Yuan; Song, Rui; Yang, Bo; Cao, Liyan; Tian, Hong; Zheng, Haixue

doi:10.1007/s00253-021-11706-z

Cited by 30 publications

(17 citation statements)

References 46 publications

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“…According to previous studies, P72 was expressed as insoluble inclusion bodies in the E. coli expression system ( Freije et al, 1993 ). Furthermore, Wan et al have developed a colloidal-gold dual immunochromatographic strip with truncated P72 using the E. coli expression system ( Wan et al, 2022 ). In addition, the truncated P72 is expressed independently in the baculovirus expression system for mAb production ( Heimerman et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies

et al. 2022

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African swine fever (ASF) is one of the highly contagious and lethal diseases among domestic pigs and wild boars. The capsid protein P72 of African swine fever virus (ASFV) is very important for the diagnosis and vaccine development. However, the epitope of the protein is not clear. In this study, capsid protein P72 was expressed in Sf9 cells along with its chaperone B602L. A total of ten monoclonal antibodies (mAbs) specific to P72 protein were developed by fusions between SP2/0 cells and spleen cells of mice immunized with the recombinant-P72&B602L proteins expressed in Sf9 cells. Four linear B cell epitopes 31SNIKNVNKSY40, 41GKPDP45, 56HLVHFNAH63 and 185ERLYE189 were identified. Biological information analysis illustrated that epitopes 31SNIKNVNKSY40, 41GKPDP45 and 185ERLYE189 were highly conserved within different ASFV strains. These findings may lead to a better understanding of the antibody-antigen interaction and provide new insights into the vaccine research and serological diagnosis of ASF.

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Section: Discussionmentioning

confidence: 99%

Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies

et al. 2022

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“…Previous studies expressed the p72 as insoluble inclusion bodies in Escherichia coli expression system (Freije et al 1993 ; Garcia et al 1998 ). Additionally, Wan et al expressed the truncated p72 through E.coli expression system (Wan et al 2022 ). Other reports produced the p72 alone in baculovirus expression system (Heimerman et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…During the review of our article, Wan et al have developed a colloidal-gold dual immunochromatographic strip with recombinant p30 and truncated p72 as described above. The sensitivity of the strip is 1:256, which is equivalent to a commercial ELISA kit (Wan et al 2022 ). Zhu et al have also utilized gold nanoparticle–labeled and acid-treated p72 to establish a lateral flow strip.…”

Section: Discussionmentioning

confidence: 99%

Development of a p72 trimer–based colloidal gold strip for detection of antibodies against African swine fever virus

Geng

Sun

et al. 2022

Appl Microbiol Biotechnol

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African swine fever virus (ASFV) causes a highly contagious and often lethal swine viral disease, and leads to tremendous economic losses to the swine industry. Unfortunately, there are no vaccines and effective antiviral agents available to prevent and control ASFV outbreaks. Therefore, it is necessary to develop simple and rapid strategies to monitor ASFV-infected pigs to restrain its spread. In the current study, ASFV capsid protein p72 was expressed along with its chaperone pB602L to form trimers in human embryonic kidney 293 (HEK293) cells. The p72 trimers were subsequently labeled with colloidal gold to develop a immunochromatographic strip. The strip showed high specificity to ASFV-positive serum and no cross-reactivity to other swine virus positive sera. Importantly, the strip showed a higher sensitivity of detecting ASFV antibodies in both positive standard serum and clinical serum samples than a commercial enzyme-linked immunosorbent assay (ELISA) kit. Taken together, these results demonstrate the strip as a reliable diagnostic tool against ASFV infection, which will be appropriate for application in prevention and control of ASFV. Key points • ASFV p72 trimers were successfully generated . • A colloidal gold strip was developed based on ASFV p72 trimers . • The strip is appropriate for detecting ASFV antibodies in the field .

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“…The wavelength of the colloidal gold-labeled rE2 particles was ~550 nm, indicating that the average size of the colloidal gold-labeled rE2 protein was ~44 nm, and thus suitable for use as a gold-labeled probe. If too large or not uniform, the gold particles tend to precipitate, leading to false-positive results ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

An Improved Immunochromatographic Strip Based on Plant-Derived E2 for Detection of Antibodies against Classical Swine Fever Virus

Sun

Yang

et al. 2022

Microbiol Spectr

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Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies

Cited by 30 publications

References 46 publications

Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies

Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies

Development of a p72 trimer–based colloidal gold strip for detection of antibodies against African swine fever virus

An Improved Immunochromatographic Strip Based on Plant-Derived E2 for Detection of Antibodies against Classical Swine Fever Virus

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