Development and Application of a Rat Ovarian Gene Expression Database

Jo, Misung; Gieske, Mary C.; Payne, Charles E.; Wheeler-Price, Sarah E.; Gieske, Joseph B.; Ignatius, Ignatius V.; Curry, Thomas E.; Ko, CheMyong

doi:10.1210/en.2004-0407

Cited by 91 publications

(88 citation statements)

References 73 publications

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“…Most studies have not explicitly quantified A2M expression in crustacean ovary, although one study reports weak expression in ovary of kuruma shrimp [52]. A2M expression has been more thoroughly studied in mammalian ovaries, where it regulates protease activity necessary for ovulation and associated tissue remodeling [53,54]. Other studies of A2M expression in crustacean tissues have found that A2M transcripts are expressed most strongly in hemocytes [52,55,56] and that expression can be induced by microbial challenge [52,55,57].…”

Section: Discussionmentioning

confidence: 99%

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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Epizootic shell disease is a poorly understood condition that has significantly affected the American lobster fishery in New England (northeastern US) since the 1990s. Here we present the results of a study to identify changes in gene expression in lobsters exhibiting symptoms of epizootic shell disease. Suppressive subtractive hybridization (SSH) was used to compare gene expression between cDNA pools from diseased (symptomatic) and apparently healthy (asymptomatic) lobsters. Subsequently, quantitative real-time polymerase chain reaction (qPCR) was used to measure expression of nine genes that were differentially-expressed in the SSH analysis, in seven tissues (muscle, gill, heart, hepatopancreas, brain, branchiostegite, gonad) dissected from individual symptomatic and asymptomatic lobsters. Expression of arginine kinase (involved in cellular energetics) was significantly decreased in muscle of symptomatic lobsters. Expression of hemocyanin (a respiratory hemolymph protein involved in oxygen transport) was highest in hepatopancreas and showed highly variable expression with a trend toward higher expression in asymptomatic individuals. 2-Macroglobulin (involved in the innate immune system) was most highly expressed in the ovary, particularly of symptomatic lobsters. The ESTs produced through this study add to the fledgling field of crustacean genomics and revealed three genes that could be further evaluated in lobsters of varying shell disease severity, molt stage, and reproductive condition, for possible implication in epizootic shell disease.

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Section: Discussionmentioning

confidence: 99%

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Tarrant

Stegeman

Verslycke

2010

Fish & Shellfish Immunology

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“…mRNA expression of several complement components, including C1qb, C3, C4, C5r1, factor h, and C1 qbp, in the ovary of the superovulated immature rat (Jo et al 2004) (http://web5.mccs.uky.edu/kolab/rogedendo.aspx). Thus, it is clear that many immune factors, including complement, are expressed in the periovulatory ovary, strongly indicating that the expression of GPI-DAF Figure 5 GPI-DAF mRNA expression is not directly controlled by P4 or PG pathways.…”

Section: Discussionmentioning

confidence: 99%

“…aspx), which was previously developed in our laboratory. The procedures used are described in detail elsewhere (Jo et al 2004). The database provides the gene expression profiles of all genes and expressed sequence tags included in the Affymetrix Rat Expression 230A and 230B GeneChips.…”

Section: Rogedmentioning

confidence: 99%

“…Recently, we have generated rOGED, which provides genome-wide temporal mRNA expression profiles in the intact ovary, granulosa cell, and residual ovarian tissue (ovarian cell types excluding granulosa cell) simultaneously (Jo et al 2004) (http://web5.mccs.uky.edu/ kolab/rogedendo.aspx). The rOGED profile revealed that hCG dramatically, yet transiently, induced GPI-DAF mRNA expression in the ovary (Fig.…”

Section: Identification Of Gonadotropin-stimulated Daf Mrna Expressiomentioning

confidence: 99%

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Decay-accelerating factor in the periovulatory rat ovary

Gieske¹,

Na²,

Koo³

et al. 2005

Journal of Endocrinology

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One of the most prominent inflammatory reactions is the activation of the complement system. The activated complement system does not distinguish between pathogens and the host cell. In order to prevent autologous complement-mediated attack, host cells express a variety of both membrane-bound and fluid-phase complement regulatory proteins which control activity of the complement cascade by acting on convertase enzymes or the membrane-attack complex. Although the process of ovulation is facilitated by the inflammatory reaction, this reaction has the potential to cause serious damage to growing follicles, ovulated follicles, and other important ovarian tissues. This study was undertaken to characterize the expression and regulation of decay-accelerating factor (DAF), a complement regulator, as a potential mediator of ovarian tissue protection from ovulatory inflammation. DNA microarray and Northern blot analyses showed that an ovulatory gonadotropin stimulus dramatically yet transiently induced DAF mRNA expression in the immature rat ovary. Northern blot and PCR analyses revealed that of the three known DAF isoforms, glycosylphosphatidylinositol (GPI)-, soluble-, and transmembrane-(TM) DAF, GPI-DAF was the predominant form. In situ hybridization localized GPI-DAF mRNA expression in the theca-interstitial cells of the periovulatory ovary. Neither the anti-progestin RU486 nor the cyclooxygenase inhibitor indomethacin significantly inhibited human chorionic gonadotropin (hCG)-induced GPI-DAF mRNA expression in vivo. In vitro theca cell culture studies indicated that hCG induces GPI-DAF mRNA expression through the protein kinase A pathway. This study suggests that gonadotropin-induced GPI-DAF may be involved in the protection of ovarian tissues from the potential attack by the complement system activated by the inflammatory response associated with ovulation.

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“…Extensive in situ hybridization analyses for brain sections were performed using genes expressed in the brain, and the results are available from the database (http://www.genepaint.org) (8). Cellular localization of genes was determined using a GFP-fused reporter system (9,10).…”

Section: Introductionmentioning

confidence: 99%

Characterization of tissue-specific mbu-3 gene expression in the mouse central nervous system

Lee

Cho²,

Kim

2008

BMB Reports

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Mbu-3 is a novel mouse brain unigene that was identified by digital differential display. In this study, expression of the gene was chased through developmental stages and the protein product was identified in the brain. The cDNA sequence was 3,995-bp long and contained an ORF of 745 AA. Database searches revealed that the chicken SST273 gene containing LRR-and Ig-domain was an mbu-3 orthologue. Tissue specificity for the gene was examined in embryos and in brains at post-natal and adult stages. During the embryonic stages, mbu-3 was localized to the central nervous system in the brain and spinal cord. In the early post-natal stages, the gene was evenly expressed in the brain. However, with aging, expression was confined to specific regions, particularly the hippocampus.

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Development and Application of a Rat Ovarian Gene Expression Database

Cited by 91 publications

References 73 publications

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Altered gene expression associated with epizootic shell disease in the American lobster, Homarus americanus

Decay-accelerating factor in the periovulatory rat ovary

Characterization of tissue-specific mbu-3 gene expression in the mouse central nervous system

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