Characterization of tissue-specific mbu-3 gene expression in the mouse central nervous system

Abstract

Mbu-3 is a novel mouse brain unigene that was identified by digital differential display. In this study, expression of the gene was chased through developmental stages and the protein product was identified in the brain. The cDNA sequence was 3,995-bp long and contained an ORF of 745 AA. Database searches revealed that the chicken SST273 gene containing LRR-and Ig-domain was an mbu-3 orthologue. Tissue specificity for the gene was examined in embryos and in brains at post-natal and adult stages. During the embryonic stages, mbu-3 was localized to the central nervous system in the brain and spinal cord. In the early post-natal stages, the gene was evenly expressed in the brain. However, with aging, expression was confined to specific regions, particularly the hippocampus.

<i>Ccdc33, </i>a Predominantly Testis-Expressed Gene, Encodes a Putative Peroxisomal Protein

“…The human CCDC33 gene is located on 15q24.1, spans a region of 99.8 kb and consists of 20 exons. Genes such as UBL7 , SEMA7A , CYP11A1 , STRA6 , ISLR and ISLR2 are present in this syngenic region in both mouse and human [Pasterkamp et al, 2003;Hsu et al, 2004;Katayama et al, 2005;Kim et al, 2008;Lee et al, 2008]. Interestingly, in mouse the neighbouring gene Cyp11a1 was demonstrated to be important for sex organ development.…”