Development and application of LC/HRPS for quantification of adenine nucleotides, creatine phosphate, and creatine in sturgeon spermatozoa

Fedorov, P. S.; Grabic, Roman; Fedorová, Ganna; Cosson, Jacky; Boryshpolets, Sergii; Dzyuba, Borys

doi:10.17221/4/2016-cjas

Cited by 8 publications

(10 citation statements)

References 30 publications

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“…In fish, creatine phosphate (CrP) is the main high-energy compound that was characterized in spermatozoa of several fish species as a complement of ATP [74,79,85]. In the last decades, many studies have demonstrated the decrease of the ATP concentration inside the fish sperm cells during the motility period [39, 67,74,76,79,85,90,91,93,100]. A more restricted number of studies have investigated the concentration of ATP related compound such as ADP, AMP, CrP, and others [74,79,85,100].…”

Section: Other Energetic Molecules Assisting Atp Maintenancementioning

confidence: 99%

“…In the last decades, many studies have demonstrated the decrease of the ATP concentration inside the fish sperm cells during the motility period [39, 67,74,76,79,85,90,91,93,100]. A more restricted number of studies have investigated the concentration of ATP related compound such as ADP, AMP, CrP, and others [74,79,85,100]. All these compounds are part of an intracellular network under control of different enzymes that are able to transfer high-energy phosphate bonds from one to another (see Figure XX in Chapter 1 of Cosson [21]), for example, the equilibrium ATP< = >ADP + Pi is catalyzed by enzymes called ATPases.…”

Section: Other Energetic Molecules Assisting Atp Maintenancementioning

confidence: 99%

“…A full evaluation of the storage of energy in fish sperm cells needs the determination not only of the internal content of ATP but also that of other energetic compounds that are able to exchange high-energy phosphate bonds able to be transferred to ADP and allow to reconstitute the intracellular ATP store. Such evaluation was established in case of sturgeon sperm [100,128] by the use of liquid chromatography combined to HPRS or in case of turbot or sea bass sperm where the adenine nucleotides' energetic balance was determined by H + -NMR and 31 P-NMR analysis, [74,79] as well as in trout by 31 P-NMR [89,90]. All these results clearly point out to the fact that ATP level can be rescued by the CrP generated by the mitochondrial metabolism.…”

Section: Evaluation Of Energetic Compounds Concentrationmentioning

confidence: 99%

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Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

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For reproduction, most fish species adopt external fertilization: their spermatozoa are delivered in the external milieu (marine-or freshwater) that represents both a drastic environment and a source of signals that control the motility function. This chapter is an updated overview of the signaling pathways going from external signals such as osmolarity and ionic concentration and their membrane reception to their transduction through the membrane and their final reception at the flagellar axoneme level. Additional factors such as energy management will be addressed as they constitute a limiting factor of the motility period of fish spermatozoa. Modern technologies used nowadays for quantitative description of fish sperm flagella in movement will be briefly described as they are more and more needed for prediction of the quality of sperm used for artificial propagation of many fish species used in aquaculture. The chapter will present some applications of these technologies and the information to which they allow access in some aquaculture species.Biological Research in Aquatic Science 2 the current knowledge with respect to (1) membrane reception of the activation signal and its transduction through the spermatozoon plasma membrane via the external membrane components such as ion channels or aquaporins; (2) cytoplasmic trafficking of the activation signal; (3) final steps of the signaling, including signal transduction to the axonemal machinery, and activation of axonemal dynein motors and regulation of their activity; (4) signaling involved in guidance processes that control the sperm/egg approach and meeting; and (5) pathways supplying energy for the short flagellar motility period of fish spermatozoa. For each step in this signaling process, quantitative methods were developed to evaluate the quality of the sperm samples that are used for aquaculture propagation of many fish species. These methods as well as examples of their usefulness for application to fish artificial reproduction are presented in the last part of this chapter. Fish sperm structure and spermatogenesisThe main traits of the flagellar mechanics of spermatozoa and the main factors that regulate their motility have been described in details in a recent review chapter by Cosson et al. [1]. Structure of fish spermatozoa: a brief presentationCompared to mammalian spermatozoa, the structure of a fish spermatozoon is qualified as "simple sperm", mostly because the flagellum structure does not include additional columns flanking the motor part (axoneme) that are present in mammal sperm. In teleost fish, spermatozoa generally have no acrosome (in contrast to chondrostean such as sturgeon), and the impenetrable chorion presents a micropyle that gives access to the membrane of the oocyte. Spermatozoa often show a spherical nucleus with homogenous, highly condensed chromatin, a nuclear fossa, a midpiece of variable size with or without a cytoplasmic channel, and one or two long flagella [2]. Moreover, fish spermatozoa can be classified into two forms, a...

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Section: Other Energetic Molecules Assisting Atp Maintenancementioning

confidence: 99%

Section: Other Energetic Molecules Assisting Atp Maintenancementioning

confidence: 99%

Section: Evaluation Of Energetic Compounds Concentrationmentioning

confidence: 99%

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Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

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“…We found cryopreservation to be associated with a 27.5% lower creatine kinase activity black-lip pearl oyster spermatozoa compared to fresh. It was also shown that enzyme activity, including creatine kinase activity, was involved in flagellar mobility and thus represents an indicator of sperm quality, as reported in sea urchin Shapiro 1985, 1989;Wallimann et al 1986;Tombes et al 1987;Wallimann and Hemmer 1994;van Dorsten et al 1997;Wallimann et al 2011), rainbow trout O. mykiss (Saudrais et al 1998) and sturgeon A. ruthenus (Fedorov et al 2017). Cryopreservation has been reported to affect enzyme activity in marine fish.…”

Section: Discussionmentioning

confidence: 84%

“…Motile spermatozoa should present a high mitochondrial respiration rate, allowing high ATP production. It was demonstrated in sea urchin Shapiro 1985, 1989;Tombes et al 1987;Walliman et al 2011;Ingermann et al 2011) that creatine-phosphate stores and creatine kinase activity are related the flagellar motility (Saudrais et al 1998;Dzyuba et al 2017;Fedorov et al 2017). van Dorsten et al (1997 showed that, in sea urchin sperm, creatine kinase activity is related to energy metabolism (O 2 consumption and ATP production).…”

Section: Discussionmentioning

confidence: 99%

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Demoy-Schneider¹,

Schmitt²,

Pennec

et al. 2018

Aquaculture

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High quality of sperm is essential to a high fertilization rate, especially post-cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O 2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O 2 consumption was 4.8 compared to 44 nanomol min −1 , ATP content was 0.72 nmol/10 9 spermatozoa in the activating medium compared to 4.54 nmol/10 9 spermatozoa, and creatine kinase activity was 9.06 × 10 −5 IU mg −1 protein compared to 12.5 × 10 −5 IU mg −1 protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process. Highlights ► Quality of the frozen/thawed black-lip pearl oyster Pinctada margaritifera spermatozoa. ► Progress to an efficient cryoconservation method for P. margaritifera spermatozoa. ► Preservation of P. margaritifera through the durable development of the pearl oyster culture in French Polynesia.

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Energetics of Fish Spermatozoa

Kholodnyy

Boryshpolets

Dzyuba

et al. 2020

Cryopreservation of Fish Gametes

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Development and application of LC/HRPS for quantification of adenine nucleotides, creatine phosphate, and creatine in sturgeon spermatozoa

Cited by 8 publications

References 30 publications

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Energetics of Fish Spermatozoa

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