Development and application of microbioassays for routine testing and biomonitoring in Argentina, Chile and Colombia

Ronco, Alicia Estela; Castillo, G. F. Torres del; Diaz-Baez, M. C.

doi:10.1007/978-1-4615-4289-6_5

Cited by 8 publications

(2 citation statements)

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“…There is little information available in most developing countries on the chemicals used and discharged and the impact of those chemicals on water resources (Dutka et al, 1996). Approaches for environmental monitoring suitable to developing countries require adequate procedures for the examination of available analytical tools to obtain appropriate and reliable information (Ronco et al, 2000). Presented here are the responses of a core battery of simple, noncommercial, standardized toxicity tests in an international quality control exercise with blind samples of unknown chemicals that accompanied the examination of environmental samples by the WaterTox Network (Diaz-Baez et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Overview of results from the WaterTox intercalibration and environmental testing phase II program: Part 1, statistical analysis of blind sample testing

Ronco

Gagnon

Diaz-Baez

et al. 2002

Environmental Toxicology

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There is an urgent need to evaluate the presence of toxicants in waters used for human consumption and to develop strategies to reduce and prevent their contamination. The International Development Research Centre undertook an intercalibration project to develop and validate a battery of bioassays for toxicity testing of water samples. The project was carried out in two phases by research institutions from eight countries that formed the WaterTox network. Results for the first phase were reported in the special September 2000 issue of Environmental Toxicology. Phase II involved toxicity screening tests of environmental and blind samples (chemical solutions of unknown composition to participating laboratories) using the following battery: Daphnia magna, Hydra attenuata, seed root inhibition with Lactuca sativa, and Selenastrum capricornutum. This battery was also used to assess potential toxicity in concentrated (10x) water samples. Results are presented for a set of six blind samples sent to the participating laboratories over a 1-year period. Analyses were performed for each bioassay to evaluate variations among laboratories of responses to negative controls, violations of test quality control criteria, false positive responses induced by sample concentration, and variability within and between labs of responses to toxic samples. Analyses of the data from all bioassays and labs provided comparisons of false positive rates (based on blind negative samples), test sensitivities to a metal or organic toxicant, and interlaboratory test variability. Results indicate that the battery was reliable in detecting toxicity when present. However, some false positives were identified with a concentrated soft-water sample and with the Lactuca and Hydra (sublethal end-point) tests. Probabilities of detecting false positives for individual and combined toxic responses of the four bioassays are presented. Overall, interlaboratory comparisons indicate a good reliability of the battery.

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Section: Introductionmentioning

confidence: 99%

Overview of results from the WaterTox intercalibration and environmental testing phase II program: Part 1, statistical analysis of blind sample testing

Ronco

Gagnon

Diaz-Baez

et al. 2002

Environmental Toxicology

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“…Fish have been historically used to assess biological effects of xenobiotics in aquatic environments. Although most studies have been conducted using species of the Holartic Region, the assessment of the sensitivity to toxicants on Neotropical species is recent (Ronco et al 2000). The atherinid teleost Odonthestes bonariensis is a widespread and characteristic fish species from the meridional sector of the 'del Plata' basin.…”

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confidence: 99%

Sensitivity of the Neotropical Teleost Odonthestes bonariensis (Pisces, Atherinidae) to Chromium(VI), Copper(II), and Cadmium(II)

Carriquiriborde

Ronco

2002

Bulletin of Environmental Contamination and Toxicology

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WaterTox bioassay intercalibration network: Results from Argentina

et al. 2000

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A series of blind samples were analyzed by our laboratory within the WaterTox Network using a battery of tests. These tests were selected according to sensitivity, easiness to perform, robustness, low cost, and representativeness of several trophic levels and consisted of acute and ( ) subchronic toxicity with the nematode Panagrellus redivivus 96 h and the coelenterate Hydra attenuata ( ) ( ) 24, 48, 72, and 96 h , acute toxicity with the microcrustacean Daphnia magna 48 h and the vascular ( ) ( ) plants Allium cepa 72 h and Lactuca sativa 96 h , and genotoxic effects with a commercial kit based ( on the Ames Salmonella typhimurium test. Data from 30 blind samples 15 pure compounds, 2 types of ) mixtures and replicate samples added with positive controls have been analyzed using the 6 selected bioassays and 12 endpoints. Results show that all assays provide relevant and complementary information. Regarding the nongenotoxic effects, within the tested concentration intervals all samples showed toxicity with at least one of the bioassays from the battery and only three samples were toxic to all. A relative toxicity rank shows that H. attenuata and D. magna are the most sensitive organisms for acute effects. H. attenuata and P. redivivus are more sensitive for sublethal effects than the vascular plants tested, except for 3 of the samples. From the 24 samples tested for genotoxic effects using the Fluctuation test 37% were mutagenic; however, serious difficulties were encountered when was run the kit for mutagenicity. The major difficulties with H. attenuata, P. redivivus, and D. magna were associated with the maintenance of stock organism cultures. The inclusion of the EC estimation of 6 blind 50 ( ) samples tested with a core battery of bioassays H. attenuata, D. magna, and L. sativa selected in the first part of the exercise, improved the design of the experiment and data quality.

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Development and application of microbioassays for routine testing and biomonitoring in Argentina, Chile and Colombia

Cited by 8 publications

References 25 publications

Overview of results from the WaterTox intercalibration and environmental testing phase II program: Part 1, statistical analysis of blind sample testing

Overview of results from the WaterTox intercalibration and environmental testing phase II program: Part 1, statistical analysis of blind sample testing

Sensitivity of the Neotropical Teleost Odonthestes bonariensis (Pisces, Atherinidae) to Chromium(VI), Copper(II), and Cadmium(II)

WaterTox bioassay intercalibration network: Results from Argentina

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