Development and application of ovine reproductive technologies: an Indian experience

Naqvi, S. M. K.; Joshi, Anil; Das, G. C.; Mittal, J. P.

doi:10.1016/s0921-4488(00)00200-5

Cited by 43 publications

(29 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The average length of the estrous cycle was comparable with observations made on other breeds [27], [28]. The differences in length of the cycle between the studies could be due to phase of the breeding season age of the sheep groups and breed differences [29].…”

Section: Discussionsupporting

confidence: 80%

“…This method predicts the estrous cycle according to the proportion of three cells types observed in the vaginal smear: epithelial cells, cornified cells and leukocytes [18]. The average progesterone levels between days of estrous cycle for both groups were within the range reported in previous studies [32], [28], [35], [19], [36]. However, there are some other studies [37], [33] in which higher progesterone concentration have been reported between days compared with the present study.…”

Section: Discussionsupporting

confidence: 75%

See 1 more Smart Citation

Reproductive Performances of Indigenous Ewes in Bangladesh

Zohara¹,

Azizunnesa²,

Islam³

et al. 2014

IOSRJAVS

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 75%

Reproductive Performances of Indigenous Ewes in Bangladesh

Zohara¹,

Azizunnesa²,

Islam³

et al. 2014

IOSRJAVS

View full text Add to dashboard Cite

“…It should be noted here that success of MOET in sheep could be variable just as it can in other farm animals. Factors such as breed, season, age, body condition and certain genetic differences all combine to produce variability in results (Armstrong and Evans 1983, Callesen et al 1996, Singh et al 1997, Delligiannis 1998, Cognie 1999, Naqvi et al 2001.…”

Section: ευχαριστιεςmentioning

confidence: 99%

“…Overcoming such difficulties is therefore important not only as a method of increasing the number of elite animals in a herd but also from an economical and practical point of view provided that limitations are understood. For example, although, cryopreservation has become an integral part of the commercial embryo transfer industry its application in sheep embryos is based on comparatively few studies (Boundy et al 1985, McKelvey and Simm 1995, Ishwar and Memon 1996 and the process continues to be improved and simplified (McGinnis et al 1993, Naqvi et al 2001. To date the information on the success rate of such cryopreservation techniques is scarce, particularly when applied to the large-scale movement of sheep embryos συστήματος των θηλυκών ατόμων (Armstrong and Evans G 1983, McKelvey και συν.…”

Section: Introductionmentioning

confidence: 99%

The use of multiple ovulation, embryo cryopreservation and embryo transfer techniques to facilitate movement of genetic material between countries

Stamataris

Deligiannis

Lainas

et al. 2018

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

The objective of the study was to evaluate the use multiple ovulation and embryo transfer techniques in an indigenous Greek dairy breed of sheep. We stimulated selected donor ewes of the Karagouniko breed to produce large numbers of embryos after the induction of multiple ovulations by gonadotropin treatment (superovulatory response). A total of 50 Greek Karagouniko ewes were synchronised into oestrus using progestagen pessaries and superovulated for embryo transfer using ovine FSH. Six days following laparoscopic insemination with fresh semen ewes were flushed surgically and embryos collected. Subsequently, the embryo recovery, along with embryo cryopreservation, embryo survival and quality were assessed. The Karagouniko donor ewes achieved a mean ovulation rate of 11.9 (SE. 0.89). The ova Recovery rate was 80,9% and 87,6% of the ova recovered being fertilised. A total of 327 (77,5%) of the viable embryos were assessed as being of sufficient quality for cryopreservation. The embryos ranged from late morulae to expanded blastocyst and were frozen via a 3-step process in 1.5 M ethylene glycol following repeated washing and trypsination. Cryopreserved embryos were frozen and then transported to Scotland, UK. There, embryos were thawed rapidly and re-hydrated via a 2 step sucrose/ethylene glycol gradient. A total of 92.4% of embryos frozen remained suitable for transfer semi-surgically into synchronized Scottish Blackface ewes. 183 embryos were transferred in total with a 66.1% survival rate. The survival rate of frozen thawed blastocysts(75%) was significantly greater than (P<0.01) that for morulae (48%). It was concluded that MOET could be successfully applied in Greek dairy breeds of sheep as a means for genetic improvement. Frozenembryos could be a successful medium for the transportation of ovine genetic material from and to Greece, however, most likely the choice of embryonic stage for cryopreservation is crucial.

show abstract

“…MOET is commercially used in sheep industry and depends on the surgical recovery of embryos, where the principal disadvantage is the limited number of times a female donor can be used (Naqvi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Relaxant effect of the essential oil of Croton nepetifolius on ovine cervix

Pereira¹,

Melo²,

Morais³

et al. 2012

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Abstract:We investigated the in vitro effect of the essential oil of Croton nepetifolius Baill., Euphorbiaceae (EOCN), on spontaneous or induced contractions of circular and longitudinal muscles from the ovine cervix during the luteal phase of the estrous cycle. The relaxant effect of EOCN was expressed as a percentage of the contraction recorded before the addition of the oil and calculated relative to the preparations exposed only to the vehicle. The IC50 (concentration of oil required to produce a 50% maximal reduction in muscle contraction) for relaxation of spontaneous contractions in circular and longitudinal muscles was signifi cantly lower than the IC50 for blockade of K + -induced contraction (27.19 µg mL -1 versus 262.72 µg mL -1 and 40.92 µg mL -1 versus 222.47 µg mL -1 , respectively). Interestingly, there was a high degree of selectivity in the action of EOCN on cervix layers concerning the inhibition of acetylcholine-induced contraction in circular (IC50 277.10 µg mL -1 ) and longitudinal (IC50 52.56 µg mL -1 ) muscles. In conclusion, EOCN is able to relax ovine cervix during the luteal phase. This work opens the perspective of applying EOCN in ovine embryo transfer.

show abstract

Development and application of ovine reproductive technologies: an Indian experience

Cited by 43 publications

References 17 publications

Reproductive Performances of Indigenous Ewes in Bangladesh

Reproductive Performances of Indigenous Ewes in Bangladesh

The use of multiple ovulation, embryo cryopreservation and embryo transfer techniques to facilitate movement of genetic material between countries

Relaxant effect of the essential oil of Croton nepetifolius on ovine cervix

Contact Info

Product

Resources

About