Development and application of oxidative stress biomarkers

Abstract

Oxidative stress may cause a wide variety of free radical reactions to produce deleterious modifications in membranes, proteins, enzymes, and DNA. Reactive Oxygen Species (ROS) generated by myeloperoxidase (MPO) can induce lipid peroxidation and also play an important role in the generation of reactive chlorinating and brominating species. As the universal biomarkers, chemical, and immunochemical approach on oxidatively modified and halogenated tyrosines has been carried out. As amido-type adduct biomarkers, chemical, and immunochemical evaluation of hexanoyl- and propanoyl-lysines, hexanoyl- and propanoyl-dopamines and phospholipids were prepared and developed for application of evaluation of novel antioxidative functional food factors. We have also involved in application of oxidatively modified DNAs such as 8-hydroxy- and 8-halogenated deoxyguanosines as the useful biomarkers for age-related diseases using both in vitro and in vivo systems. Application of these oxidative stress biomarkers for novel type of functional food development and recent approach for development of novel evaluation systems are also discussed.

Selenium attenuates diclofenac‐induced testicular and epididymal toxicity in rats

“…Moreover, an elevated level of NO—a reactive free radical—is known to induce nitrosative stress that subsequently damages cellular lipids, nucleic acids and proteins after diminishing the antioxidant defence mechanisms (Moldogazieva, Mokhosoev, Feldman, & Lutsenko, 2018; Taysi, Tascan, Ugur, & Demir, 2019). Increased MPO activity is associated with the excessive free radical generation and oxidative stress (Ndrepepa, 2019; Osawa, 2018). The current study showed that diclofenac administration alone increased MPO activity and NO level in testes and epididymis of treated rats, thus implying the involvement of nitrosative and oxidative stress in the toxicity.…”

The chronic effects of cyanide on oxidative stress indices in the domestic chicken (Gallus domesticus L.)

“…Some of its biotoxic effects could be mediated through the attack of generative reactive oxygen species on some target organs such as the liver, kidney, heart, and brain. When the effects of free radicals are not mitigated in cells, oxidative stress occurs (He et al, 2017;Osawa, 2018). Some of the biomarkers of oxidative stress are catalase, malondialdehyde, and superoxide dismutase (Okolie & Iroanya, 2003;Petkovic et al, 2018).…”

Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study

“…(3,4) While WBCs are essential for immunity, they are also major endogenous oxidants that may cause chronic inflammation. (5)(6)(7)(8) WBCs are related to various disorders, including arteriosclerosis, (9)(10)(11) neurodegenerative diseases, (12) autoimmune diseases, (13) and cancer, (14,15) but the relationships between each disease and their ability to produce ROS and hROS are not well understood. (9)(10)(11)(12)(13)(14)(15) We have developed monitoring systems for ROS (CL-O 2…”