Development and application of polymeric monolithic stationary phases for capillary electrochromatography

Hilder, Emily F.; Švec, František; Fréchet, Jean M. J.

doi:10.1016/j.chroma.2004.04.057

Cited by 212 publications

(115 citation statements)

References 130 publications

(222 reference statements)

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“…Up till now, both organic polymer [3][4][5] and silica-based [6][7][8][9][10] monoliths have been developed. Polymeric monolithic stationary phases have the advantage of simple polymerization procedure and easy tuning of porosity and surface chemistry, but they suffer from shrinking or swelling when exposed to different organic mobile phases, leading to lack of mechanical stability.…”

Section: Introductionmentioning

confidence: 99%

Hybrid organic-inorganic phenyl monolithic column for capillary electrochromatography

Liang

Zhang

et al. 2005

Electrophoresis

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A novel hybrid organic-inorganic silica-based monolithic column possessing phenyl ligands for reversed-phase (RP) capillary electrochromatography (CEC) is described. The monolithic stationary phase was prepared by in situ co-condensation of tetraethoxysilane (TEOS) with phenyltriethoxysilane (PTES) via a two-step catalytic sol-gel procedure to introduce phenyl groups distributed throughout the silica matrix for chromatographic interaction. The hydrolysis and condensation reactions of precursors were chemically controlled through pH variation by adding hydrochloric acid and dodecylamine, respectively. The structural property of the monolithic column can be easily tailored through adjusting the composition of starting sol solution. The effect of PTES/TEOS ratios on the morphology of the created stationary phases was investigated. A variety of neutral and basic analytes were used to evaluate the column performance. The CEC columns exhibited typical RP chromatographic retention mechanism for neutral compounds and had improved peak shape for basic solutes.

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Section: Introductionmentioning

confidence: 99%

Hybrid organic-inorganic phenyl monolithic column for capillary electrochromatography

Liang

Zhang

et al. 2005

Electrophoresis

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“…Organic polymer-based monolithic column prepared by in situ polymerization has been developing rapidly over the last two decades [23][24][25][26]. The advantages, including flexible synthesis, adjustable porosity, fast mass transport, variety of surface chemistry, pH stability and biocompatibility, make monolith an ideal alternative medium to the traditional silica stationary phases in microscale bio-analysis [24].…”

Section: Introductionmentioning

confidence: 99%

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Wang

Duan

et al. 2011

J of Separation Science

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To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr 41 -IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-coethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr 41 -IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr 41 -IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection. IntroductionIn recent years, protein phosphorylation has gained much attention because of its significant roles in cellular signal transduction processes [1,2]. However, the amount of phosphorylated proteins in eukaryotic cells often exists in substoichiometry level, which makes the identification of phosphorylation sites challenging [3]. To date, mass spectrometry (MS) analysis has become an effective and commonly used method for characterization of phosphoproteins [4]. To reduce the interference of non-phosphopeptides, selective enrichment strategies, such as immobilized metal ion affinity chromatography (IMAC) [5][6][7], metal oxide affinity chromatography (MOAC) [8,9], and ion exchange chromatography [10][11][12][13], are necessary before MS analysis.Recently, chip-based system has been introduced as a promising platform for phosphopeptide analysis, due to the benefits of low sample amount, high throughput, and easy integration [14]. Several microfluidic devices have been developed by integrating phosphopeptide enrichment with other functions, such as protein alkylation and reduction [15], protein digestion [16], peptide separation and on-line interface for MS instrument [17][18][19][20], which have shown great potentials in the field of phosphoproteome research. However, in these microchip devices, phosphopeptide enrichment components are usually fabricated by packing functionalized particles, such as TiO 2 or IMAC beads, into m...

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“…Monoliths are the fourth generation chromatography material [3], succeeding beads, porous particle-based columns and membrane adsorbers. In the last two decades, numerous articles describing preparation, properties and applications of monolithic columns were published [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. In contrast, this review focuses on the applied aspect of monoliths and describes recent applications of monoliths for the purification of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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Development and application of polymeric monolithic stationary phases for capillary electrochromatography

Cited by 212 publications

References 130 publications

Hybrid organic-inorganic phenyl monolithic column for capillary electrochromatography

Hybrid organic-inorganic phenyl monolithic column for capillary electrochromatography

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Monoliths in Bioprocess Technology

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