Development and characterization dual responsive magnetic nanocomposites for targeted drug delivery systems

Abstract

A drug delivery system based on dual responsive units was developed. An appealing pH- and thermo-responsive triblock terpolymer as the drug carrier was synthesized by RAFT polymerization of N-isopropyl acrylamide and methacrylic acid monomers using PEG-RAFT agent. The FeO magnetic nanoparticles were synthesized by co-precipitation of Fe salts. Synthesized samples were characterized by FT-IR, XRD, GPC, SEM and TEM. The dual responsive behaviour and self-assembly of the triblock terpolymers in aqueous solution were investigated using UV-vis transmittance and DLS. Based on the results of DLS and TEM, the average size of micelles was 170, 125 and 30 nm. The triblock terpolymer was used as a chemotherapy drug carrier and doxorubicin as a model drug. The release rate of the drug at two different temperatures (37 °C and 42 °C) and pHs (5.8 and 7.4) was studied. The in vitro cytotoxicity assay of free doxorubicin and drug-loaded magnetic nanoparticles was studied. The MTT assay exhibited that these polymers are biocompatible and no toxicity. As well, IC of the DOX-loaded triblock terpolymer in MTT test demonstrated that these systems could be suitable for the treatment of cancer.

Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the transfection of pro-BDNF into neural stem cells

“…Some of non-viral carriers were designed and developed to overcome the challenges of ineffective DNA-endosomal capture and release from the carrier vector into the nucleus, and represent a promising methods for gene delivery purposes [10]. Superparamagnetic iron oxide NPs (SPIONs) are widely used for biomedical applications, such as target gene delivery systems and gene therapy, It can be used also in cancer chemotherapy as drug carrier after coated with biocompatible polymers [11,12] SPIONs with unique magnetic properties are being actively investigated as magnetic resonance imaging contrast agents [12], It can be properly designed and engineered to reach their target tissue with less nonspecific cellular interactions [13,14]. In addition, SPIONs showed a high labeling efficiency in different mammalian cell types [15].…”

Thermo/pH dual-responsive micelles based on the host–guest interaction between benzimidazole-terminated graft copolymer and β-cyclodextrin-functionalized star block copolymer for smart drug delivery

“…In this equation, OD t and OD b are the optical density values of surviving cells treated with test group and blank control, respectively. The results represent the average values of three measurements [ 31 ].…”

Chemotherapy of Breast Cancer Cells Using Novel pH-ResponsiveCellulose-Based Nanocomposites

“…The relative cytotoxicity of nanocarriers, free DOX, and DOX-loaded nanocarriers were assessed against the MCF7 after 48 hours exposure time by MTT assay method based on the following method. 12,36 The MCF7 cells were cultured in Roswell Park Memorial Institute 1640 growth medium (RPMI 1640 medium; Gibco BRL Life Technologies) containing 10% fetal bovine serum, 100 U/mL penicillin and 100 µg/mL streptomycin and incubated at 37°C in an atmosphere of 5% CO2 and 95% air with more than 95% humidity in the incubator to allow the cells to grow and attach to the bottom of each well. Then the medium was changed with fresh culture growth medium having DOX and DOX-loaded nanocarriers with varying concentrations of the drugs (25, 50, 100 and 200 µg/mL).…”