Development and characterization of 12 microsatellite markers from the deep‐sea hydrothermal vent siboglinid Riftia pachyptila

Abstract

Ecological processes at deep-sea hydrothermal vents on fast-spreading mid-ocean ridges are punctuated by frequent physical disturbance, often accompanied by a high occurrence of population turnover. To persist through local extinction events, sessile invertebrate species living in these geologically and chemically dynamic habitats depend on larval dispersal. We characterized 12 polymorphic microsatellite loci from one such species, the siboglinid tubeworm Riftia pachyptila. All loci conformed to Hardy-Weinberg expectations without linkage (mean H(E) = 0.9405, mean N(A) = 20.25). These microsatellites are being employed in the investigation of spatial and temporal population genetic structure in the eastern Pacific Ocean.

Panmixia in a Fragmented and Unstable Environment: The Hydrothermal Shrimp Rimicaris exoculata Disperses Extensively along the Mid-Atlantic Ridge

“…Both the mean number of alleles per locus (3.55–17.91) and gene diversity (0.66–0.71) are within the values found for other hydrothermal vent organisms (e.g. [12] , [50] ). However, in another hydrothermal vent shrimp species, Chorocaris sp, similar allelic richness (2–16) and higher variability in gene diversity (0.17–0.82) were detected using microsatellite markers, on the basis of a much smaller sample size [51] .…”

Genetic diversity and demographic instability in Riftia pachyptilatubeworms from eastern Pacific hydrothermal vents

“…Their mitochondrial haplotype diversities range from a minimum of 0.331 in R. pachyptila to a maximum of 0.960 in another annelid, with a mean of 0.615 across 11 species [summarized in Figure five of reference 15]. Examinations of nuclear allozymes, AFLPs and DNA microsatellites from limited portions of R. pachyptila 's range [ 24 , 28 , 29 ] suggests that its low COI variation may be anomalous, resulting perhaps from a mitochondrial selective sweep. Nevertheless, allozymes, AFLPs and microsatellites exhibit mutation rates that do not compare to mitochondrial DNA sequences, and they might experience different selective constraints.…”

Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents

“…Microsatellite markers have been developed for three other deep-sea hydrothermal-vent-endemic organisms (Riftia pachyptila: Fusaro et al 2008; Branchipolynoe seepensis: Daguin and Jollivet 2005; Bathymodiolus childressi: Carney et al 2006). I. nautilei microsatellite markers are being deployed on I. nautilei populations in the western Pacific to explore connectivity within and among Manus, Fiji, and Lau Basins.…”

“…Biomass-dominant taxa endemic to vent systems are key targets for understanding population structure and connectivity at vents (e.g., Hurtado et al 2004;Shank and Halanych 2007;Fusaro et al 2008). Commercial grade polymetallic sulfide deposits are often associated with hydrothermal vents, making some vents candidates for mining operations (Rona 2003).…”