“…Thus, the human skin commensal Staphylococcus epidermidis , Cutibacterium acnes , and Malassezia furfur , and the transient pathogenic Staphylococcus aureus , grown over keratinocytes seeded on fibroblast-embedded fibrin matrix dermis in a Transwell ® insert, show that oil exceeding physiological levels leads to development of C. acnes or M. furfur , responsible for inflammatory acne or seborrheic dermatitis and pityriasis versicolor, respectively [ 62 ]. Moreover, the imbalance of C. acnes and peroxidized squalene treatment in a 3D model with a single epidermal layer is able to reproduce acne-prone skin, with an increase in inflammatory factors, decrease in claudin-1, and reduced epidermis integrity [ 63 , 64 ]. Although these models are a good initial approach to study cutaneous microbiome and pathologies, the addition of other microorganisms from the skin and sebaceous glands, flow, or other mechanical requirements for the model would more closely represent human skin, allowing permeability studies or simulating different pathologies caused by alterations in the microbiota.…”