Development and characterization of erythrosine nanoparticles with potential for treating sinusitis using photodynamic therapy

Garapati, Chandrasekhar; Clarke, Brandon W.; Zadora, Steven; Burney, Charles; Cameron, Brent D.; Fournier, Ronald L.; Baugh, Reginald F.; Boddu, Sai H. S.

doi:10.1016/j.pdpdt.2015.01.005

Cited by 22 publications

(9 citation statements)

References 26 publications

(20 reference statements)

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“…The significantlydecreased value of viscosity measured in PLGA-ER solutions with respect to solutions PLGA-ND and PLGA-MB may hint at secondary, e.g. hydrophobic, interactions between the PS and the fibre-forming polymer, as indirectly observed in ER-loaded PLGA nanoparticles 16 . Such secondary interactions between ER and PLGA are expected to compromise the polymer chain entanglements leading to a decrease in solution viscosity, as observed previously with different polymer and additive formulations 37,38 .…”

Section: Characterisation Of Electrospinning Solutionsmentioning

confidence: 85%

“…MB has also been shown to reduce the viability of microorganisms in biofilms grown on discs of bovine dentin upon LED light activation 15 . Similarly, ER-loaded nanoparticles exhibited significantly higher antimicrobial efficacy against S. aureus cells than pure PS in solution 16 . Given their porous microarchitecture, large surface area and biomimetic fibrous characteristics, we suggest that further improvements in the delivery of PS could be achieved using fibrous scaffolds, especially to integrate sustained antimicrobial activity with soft tissue repair capability.…”

Section: Introductionmentioning

confidence: 92%

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Photodynamically Active Electrospun Fibers for Antibiotic-Free Infection Control

Contreras

Raxworthy

Wood

et al. 2019

ACS Appl. Bio Mater.

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Antimicrobial biomaterials are critical to aid in the regeneration of oral soft tissue and prevent or treat localised bacterial infections. With the rising trend in antibiotic resistance, there is a pressing clinical need for new antimicrobial chemistries and biomaterial design approaches enabling on-demand activation of antibiotic-free antimicrobial functionality following an infection that are environment-friendly, flexible and commercially-viable. This study explores the feasibility of integrating a bioresorbable electrospun polymer scaffold with localised antimicrobial photodynamic therapy (aPDT) capability. To enable aPDT, we encapsulated a photosensitiser (PS) in polyester fibres in the PS inert state, so that the antibacterial function would be activated on-demand via a visible light source. Fibrous scaffolds were successfully electrospun from FDA-approved polyesters, either poly( -caprolactone (PCL) or poly[(rac-lactide)-co-glycolide] (PLGA) with encapsulated PS (either methylene blue (MB) or erythrosin B (ER)). These were prepared and characterised with regards to their loading efficiency (UV-Vis spectroscopy), microarchitecture (SEM, porometry and BET (Brunauer-Emmett-Teller) analysis), tensile properties, hydrolytic behaviour (contact angle, dye release capability, degradability) and aPDT effect. The electrospun fibres achieved an ~100 wt.% loading efficiency of PS, which significantly increased their tensile modulus and reduced their average fibre diameter and pore size with respect to PS-free controls. In vitro, PS release varied between a burst release profile to limited release within 100 hours depending on the selected scaffold formulation, whilst PLGA scaffolds displayed significant macroscopic shrinkage and fibre merging following incubation in phosphate buffered saline solution. Exposure of

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Section: Characterisation Of Electrospinning Solutionsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 92%

Photodynamically Active Electrospun Fibers for Antibiotic-Free Infection Control

Contreras

Raxworthy

Wood

et al. 2019

ACS Appl. Bio Mater.

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“…The anti-biofilm effect on S. mutans , F. nucleatum , A. actinomycetemcomitans and P. gingivalis was well supported by the findings of SEM and CLSM images. Similarly, some recent studies have indicated the enhanced antimicrobial activity when pure drugs are incorporated into nanoparticle carriers [ 35 , 36 ]. Hence, nanotherapy may bring clinical benefits to the patients suffering from oral biofilm-induced infections and inflammation (e.g.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

Leung

Seneviratne

et al. 2016

Nanomaterials

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Scutellaria baicalensis (SB) is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX). Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB) and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB) as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX) of Nano-SB and Nano-CHX at 9:1 (w/w) ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S.) mutans, S. sobrinus, Fusobacterium (F.) nucleatum, and Aggregatibacter (A.) actinomycetemcomitans (MIC 50 μg/mL) at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P.) gingivalis (MIC 12.5 μg/mL) at 24 h that was supported by the findings of both scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM). This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.

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“…Garapati et al (48) investigated the role of erythrosine nanoparticles in PDT. They offer detailed and thorough methodology regarding the development of their erythrosine-loaded PLGA (biodegradable polymer) nanoparticle delivery system.…”

Section: Pre-clinical Studies (Laboratory)mentioning

confidence: 99%

Photodynamic Therapy as a New Treatment for Chronic Rhinosinusitis - A Systematic Review

Kaura

Shukla

Lamyman

et al. 2021

Turk Arch Otorhinolaryngol

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This review examines the latest evidence for photodynamic therapy (PDT) in treating chronic rhinosinusitis. MedLine, EMBASE and TRIP Database searches were conducted using the terms: "photodynamic" or "phototherapy" or "photo" and "sinusitis" or "rhinosinusitis," date range January 2000 to May 2020. A total of 192 records were initially identified, after duplicates and exclusions, 9 full papers and 3 abstracts were included. All study types including in-vitro, animal and human studies were evaluated. Whilst there is in-vitro evidence for the efficacy of PDT's bactericidal effect on drug resistant bacteria and biofilm viability, there are few clinical studies. PDT is a promising area of research, but larger, focused studies looking at the safety, delivery, efficacy, and patient selection are required before it can be considered a viable treatment for CRS.

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Development and characterization of erythrosine nanoparticles with potential for treating sinusitis using photodynamic therapy

Cited by 22 publications

References 26 publications

Photodynamically Active Electrospun Fibers for Antibiotic-Free Infection Control

Photodynamically Active Electrospun Fibers for Antibiotic-Free Infection Control

Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

Photodynamic Therapy as a New Treatment for Chronic Rhinosinusitis - A Systematic Review

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