Development and characterization of EST-SSR markers for <i>Pinus thunbergii</i>

Mukasyaf, Aziz Akbar; Tamura, Miho; Yamaguchi, Rimi; Teshima, Kenjiro; Watanabe, Atsushi

doi:10.1080/13416979.2021.1964152

Cited by 3 publications

(1 citation statement)

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“…DNA analysis was conducted using seven EST-SSR markers listed in Table 1, which include Pt_ESTSSR_36750, Pt_ESTSSR_38962, Pt_ESTSSR_34351, Pt_ESTSSR_27622, Pt_ESTSSR_24700, Pt_ESTSSR_34853, and Pt_ESTSSR_33385. The genotyping processes for these EST-SSR markers were carried out according to the identical protocols as described in our earlier studies [6], ensuring consistency and comparability of the genetic data obtained. The genetic data were analyzed by Structure, a software widely used for assessing individual-based genetic structure through Bayesian methods.…”

Section: Methodsmentioning

confidence: 99%

Biogeography: A Case in Spatial Distribution Pattern of 21 Pinus thunbergii Populations in Japan

Mukasyaf

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Biogeography is study of spatial distribution of organisms that has always been a source of fascination for naturalists, ecologists, and scientists. Evaluating genetic differentiation at various spatial scales provides valuable biogeographical insights relate to genetic structure of the species within the populations. The Expressed Sequence Tag - Simple Sequence Repeat, EST-SSR, marker used in these studies to gain insights into the efficiency of this marker. The objective of these studies was to provide a deeper understanding of the genetic diversity, structure, and phylogeographic history of this species, thus filling an important knowledge gap in the field of forest genetics and biogeography. According to historical context that cultivation and domestication of P. thunbergii have been taking place for centuries. Consequently, the spatial distribution pattern of 21 P. thunbergii populations cannot be divorced from the influence of human activities. It is provides valuable information about the factors that have led to the observed distribution pattern. Genetic differentiation has revealed distinct differences between two predecessor clusters, West-South Ward and East-North Ward. These clusters may hold insights into the biogeographical history of P. thunbergii in Japan, shedding light on its evolutionary past and how it has adapted to its environment over time.

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Section: Methodsmentioning

confidence: 99%