Development and characterization of genic SSR markers for mungbean (Vigna radiata (L.) Wilczek)

Gupta, Sudhir Kumar; Bansal, Ravindra; Gopalakrishna, T.

doi:10.1007/s10681-013-0993-0

Cited by 55 publications

(42 citation statements)

References 37 publications

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“…A similar result was reported in Tsukazaki et al, (AT 25.6%, GC 0.5%) [10] and, based on transcriptome sequencing, the same result that the AT repeat motif was more common than GC, has been obtained in many plant species, such as bulb onion, radish, mung bean, pear, Levant cotton, and so on [16,17,[25][26][27]. Although a large number of the AT motif emerged from the transcriptome sequences, it has not typically been used to develop molecular markers because of its self-complementary nature, which leads to the formation of dimmers [28].…”

Section: Frequency and Distribution Of Genic Ssrs In The Welsh Onion supporting

confidence: 87%

“…In contrast to other plants, the Na and PIC values for the Welsh onion were lower than those reported in pear buds [36], 9.42 vs. 0.75, respectively. However, the values were higher than those for sweet potato (2.94 and 0.35, respectively) [25], cowpea (3.06 and 0.53, respectively) [37], and mung bean (3 and 0.34, respectively) [16].…”

Section: Validating the Effectiveness Of Genic Ssr Markers Among 22 Amentioning

confidence: 65%

“…In recent years, a large number of genomic SSR markers have been developed in the Welsh onion [14,15], but genic SSR markers have largely been ignored until recently; Tsukazaki et al, described transcriptome shotgun assembly (TSA)-derived markers in Japanese bunching onion [10]. Genic SSRs have distinct advantages compared to genomic SSRs in terms of reducing the time and cost of developing SSR markers, and they are more useful as genetic markers because they are part of the transcriptome and represent the variation in the expressed portion of the genome [16]. Recently, hundreds of genic SSR markers, even SNPs (single nucleotide polymorphisms), have been developed by analyzing large amounts of ESTs in the bulb onion (A. cepa L.) cDNA libraries [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.)

et al. 2015

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Transcriptome analysis is an efficient way to explore molecular markers in plant species, for which genome sequences have not been published. To address the limited number of markers published for the Welsh onion, this study found 6486 loci of genic simple sequence repeats (SSR), which consisted of 1-5 bp repeat motifs, based on next-generation sequencing (NGS) technology and the RNA-Seq approach. The most abundant motif was mononucleotide (52.33%), followed by trinucleotide (31.96%), and dinucleotide (14.57%). A total of 2525 primer pairs were successfully designed, and 91 out of 311 tested primers were polymorphisms. Overall, 38 genic SSR markers were randomly selected to further validate the degree of genetic diversity, and 22 genic SSR markers (57.89%) showed high levels of polymorphism. The average polymorphism information content (PIC) value and the number of alleles (Na) were 0.63 and 5.27, respectively, and the unweighted pair-group method with arithmetic average (UPGMA) cluster analysis grouped

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Section: Frequency and Distribution Of Genic Ssrs In The Welsh Onion supporting

confidence: 87%

Section: Validating the Effectiveness Of Genic Ssr Markers Among 22 Amentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

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Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.)

et al. 2015

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“…Malone et al (2007), comentam que o PIC está relacionado com o número de alelos, que, por sua vez, está diretamente associado à divergência genética e ao número de genótipos em estudo. Gupta et al (2014) a identificação de genótipos superiores com base na divergência genética é a estratégia mais adequada para iniciar um programa de melhoramento. Sendo importante ressaltar que é mais efetivo realizar cruzamentos entre genótipos altamente divergentes, no entanto com um bom potencial produtivo.…”

Section: Resultsunclassified

Genetic variability in early-cycle erect cowpea analysed with RAPD and ISSR markers

Dias

Bertini

Silva

et al. 2015

Revista Ciência Agronômica

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RESUMO -ABSTRACT -Knowledge of genetic variability is of fundamental importance in the identification of superior genotypes when starting a breeding program for crops in general. The aim was to evaluate genetic variability in 38 genotypes of early-cycle, erect cowpea using RAPD and ISSR markers. The material came from the Germplasm Bank of the Federal University of Ceará and the cowpea breeding program of Embrapa Meio Norte. The DNA extractions were carried out following the Doyle and Doyle protocol. Ten RAPD primers generated 71 polymorphic bands (88.75%) with 7.1 markers per primer, whereas nine ISSR primers produced 47 polymorphic bands (75.81%) with 5.22 markers per primer. With cluster analysis, the EC-748 genotype was seen to be distinct from the others, and the AU 94-MOB-816 and UCR 95-701 genotypes showed the highest degree of similarity among those under evaluation. The RAPD and ISSR markers were efficient in detecting polymorphism between genotypes of early-cycle, erect cowpea.

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“…Using simple sequence repeat markers (SSR) the genetic relationship among 20 mung bean genotypes was studied and among that 78% genic SSR markers showed polymorphism and the number of alleles ranged from two to six with average polymorphic information content (PIC) value of 0.34. The wild and cultivated genotypes were separated clearly into groups with cluster analysis (Gupta et al, 2014). To study molecular diversity among 21 mung bean genotypes, Bharti et al (2013) used sequence-related amplified polymorphism (SRAP) markers and out of 29 SRAP primers six primers were more useful in fingerprinting mung bean genotypes.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

Alghamdi

Al-Faifi

Migdadi

et al. 2017

IJAB

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Grain legumes are grown for their high proteins and biological nitrogen fixation ability. In this study, 3 mung bean, 4 lentil and 20 faba bean genotypes from the Kingdom of the Saudi Arabia were evaluated for genetic diversity. There were significant differences in the morphological and molecular characteristics in the tested mung bean (Vigna radiata L.), lentil (Lens culinaris Medik.) and faba bean (Vicia faba L.) genotypes. The sequence-related amplified polymorphism (SRAP) and amplified fragments length polymorphism (AFLP) markers exhibited a considerable genetic diversity among the tested genotypes of all three legume crops. The genotypes differed for plant height, primary branches, pod length, pods per plant, grain per pod and 100-grain weight. All the tested genotypes of mung bean, lentil and faba bean differed in the number of alleles. Maximum number of alleles per primer combination was 112 in mung bean, 72 in lentil and 126 in faba bean.Polymorphism percentage of all the tested genotypes of the mung bean and lentil was 100% and in faba bean, it ranged from 97 to 100%. Existence of genetic diversity of these tested mung bean, lentil and faba bean genotypes offers opportunities to exploit favourable alleles for use in the breeding program aimed at yield improvement.

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Development and characterization of genic SSR markers for mungbean (Vigna radiata (L.) Wilczek)

Cited by 55 publications

References 37 publications

Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.)

Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.)

Genetic variability in early-cycle erect cowpea analysed with RAPD and ISSR markers

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

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