Development and characterization of integrating sphere for photometry and radiometry measurement

Wibawa, Bambang Mukti; Mujahid, Abdul Al; Mindara, Jajat Yuda; Panatarani, Camellia; Joni, I Made; Siregar, Rustam E.

doi:10.1063/1.4820312

Cited by 3 publications

(3 citation statements)

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“…Thus BaSO 4 was combined with Polyethylene Glycol (PEG) or white paint (Nippon Paint Vinilex White). Addition of the PEG or white paint aim to strengthen the mechanical property of the coating without reducing the reflecting characteristic of the BaSO 4 [1]. In addition, binding material was added to enhance BaSO 4 durability.…”

Section: Integrating Sphere Coatingmentioning

confidence: 99%

“…The efficacy can be measured more accurate using an IS since it capable on collecting all light rays produced by LED. Thus the accuracy of the efficacy measurement determined by the quality of the IS such as size, reflectance of its interior coating and port area (LED and sensor entry ports) [1,2]. Hanselaer, et al [3] reported the development of typical hemispherical radiation of high-power LEDs to increase the accuracy of the flux determination using a custom-made integrating sphere by the use of an external spectral irradiance standard and an optimized spectral irradiance detection head was combined with a very particular port geometry and a minimized baffle area.…”

Section: Introductionmentioning

confidence: 99%

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Integrating sphere design for characterization of LED efficacy

Mujahid¹,

Panatarani²,

Maulana³

et al. 2016

AIP Conference Proceedings

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Abstract. The integrating sphere (IS) is one of the most important device in characterization of illuminance of a light source, such as CFL, LED etc. to obtain their efficacy. IS is a hollowed sphere with its interior covered with a diffuse white reflective coating where its accuracy of the measurement is highly affected by reflectance of its interior coating. This paper report the preparation of inner surface coating of the IS with inner diameter of 25 cm attempt to create a durable and highly reflective interior coating by combining BaSO 4 with a binding material (either Polyethylene Glycol (PEG) or white paint). The various inner surface coating mixture vary in weight % ratio of BaSO 4 :PEG or BaSO 4 :white paint were investigated. The results show that the inner surface coating mixture of BaSO 4 :PEG (99.8:0.2) has highest reflectance compared to others mixture. The IS with best mixture was calibrated with white LED and resulted an average sphere multiplier (M) was 8.7, and average reflectance ( ) was 0.90. The result of the relative error of luminescence measurement using calibrated M and is 6.7 %. It is concluded that the developed IS produced lower allowed error compared to the commercially available IS. However at lower wavelength shows lower intensity compare to the available datasheet of the LED under investigation.

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Section: Integrating Sphere Coatingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrating sphere design for characterization of LED efficacy

Mujahid¹,

Panatarani²,

Maulana³

et al. 2016

AIP Conference Proceedings

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“…QDs and dyes can be further characterised using emission, excitation, and PLQY which can be measured on a spectrometer with an integrating sphere 136 . A laser excitation source is directed into an integrating sphere which allows for collection of light from all directions.…”

Section: Emission Excitation Plqymentioning

confidence: 99%

Comparison of quantum dots and dyes for Förster resonance energy transfer based aptamer sensors

Mare

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Bio-sensing has enabled the detection and identification of many analytes, including molecules, viruses, diseases, cancers, pollutants, and pesticides. One type of biosensor involves the use of fluorescent dyes capable of Fӧrster Resonance Energy Transfer (FRET) which, although effective in signalling, can be sensitive to environmental changes such as pH, temperature, and ionic concentrations due to their organic nature. In contrast, inorganic quantum dots are resistant to these environmental changes while also producing a controlled and specific signal through its tuneable band gap and narrow emission. Aptamer-based quantum dot biosensors are therefore a promising alternative to organic dye sensors due to the specificity of aptamers and valued properties of the quantum dots. A combination of green and red fluorophores with attached complementary aptamers allows a FRET signal to be observed when there is no target present, and no FRET signal observed when the target is present.Here, the selectivity and specificity of novel InP/Zns QD-thrombin aptamer conjugates are investigated and compared with Cyanine3 (Cy3) and Cyanine5 (Cy5) in response to a thrombin protein target. Firstly, the fluorophores are synthesised and characterised. Secondly, four systems are tested for their suitability for FRET, QD-QD, QD-dye, dye-QD, and dye-dye. Thirdly, the suitable systems are tested with the thrombin target for detection through FRET interruption, finding that some detection is observed at higher nM concentrations of thrombin within the QD-QD and QD-dye systems, particularly with lower concentrations of fluorophore. Fourthly, interactions between the green donor QD and thrombin protein are investigated as a possible reason behind the lack of detection in some systems. Finally, the QD-dye system is used with a second target, ATP, and a second dye, Texas Red, to demonstrate the applicability of the overall system type with a range of targets. While the biosensor systems tested were not reliable for thrombin detection, knowledge was gained about the differences between QDs and dyes within the systems tested, and the use of non-toxic InP/ZnS QDs within a FRET-based aptamer system shows promise for future diagnostic use.

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