Development and characterization of lutein-loaded SNEDDS for enhanced absorption in Caco-2 cells

Abstract

A self-nanoemulsifying drug delivery system (SNEDDS) has been developed for enhanced oral bioavailability of lutein. Its permeation enhancement has been evaluated using monolayers of Caco-2 cells. SNEDDS is composed of a mixture of Lexol® and Emulmetik® 900, Labrasol®, and Tween 80 as oil, surfactant and co-surfactant, respectively. Upon dilution of lutein-loaded SNEDDS with water, a nanoemulsion was obtained in <10 s with spherical droplets of 40-150 nm in diameter. The zeta potential was in the range of -19 to -32 mV. Increasing the ratio of surfactant to co-surfactant decreased the mean droplet size. Dissolution studies showed that lutein was released rapidly (<5 min) from SNEDDS into 0.1 N HCl and pH 6.8 phosphate buffer solution without any aggregation. In vitro studies using Caco-2 cells revealed that lutein-loaded SNEDDS showed shorter lag time and greater (2-fold) cellular accumulation compared with the lutein dispersion.

Development and Characterization of Indomethacin-Loaded Mucoadhesive Nanostructured Lipid Carriers for Topical Ocular Delivery

“…The shake-flask method was employed to evaluate the dissolution profile of IND-NLCs formulations [15]. IND is a poorly water-soluble drug.…”

Zinc‐loaded whey protein nanoparticles prepared by enzymatic cross‐linking and desolvation

“…Similarly, incubation of WPI-TG-zinc nanoparticles for 48 h showed significant decrease in the cellular viability compared to control (P < 0.05). However, the cell viability values of Caco-2 cells were more than 80% after incubation for 24 and 48 h within the concentration of test range, which were considered as non-toxic to Caco-2 cells (Niamprem et al, 2013).…”

Chitosan-sodium alginate-fatty acid nanocarrier composite: lutein bioavailability, absorption pharmacokinetics in diabetic rat and protection of retinal cells against H2O2 induced oxidative stress in vitro

“…In addition, since the size of the LNCs was 98 nm, it was found to easily cross the physiological (intestinal barrier and blood-retinal barrier) barriers. This could be due to the higher interfacial area of smaller particle size of LNCs, which led to maximum absorption of lutein with slow and controlled release compared to bulk materials and mixed micelles (Niamprem, Rujivipat, & Tiyaboonchai, 2014;Ranganathan et al, 2019). This could be the possible reason for the higher plasma and RPE levels of lutein from LNCs than the micellar lutein.…”

“…In the present study, LNCs displayed higher (79%) RPE-19 cell viability than micellar lutein, indicating greater cellular uptake and scavenging intracellular ROS. The higher e cacy of LNCs, the smaller particle size and slow and controlled lutein release from LNCs compared to micelles could be the reason for higher cell viability (Mukerjee & Vishwanatha, 2009;Niamprem et al, 2014).…”