Development and characterization of microsatellite DNA primers for ferruginous pygmy‐owls (Glaucidium brasilianum)

Abstract

Ten polymorphic microsatellite loci were isolated and characterized for the ferruginous pygmy owl (Glaucidium brasilianum). Observed and expected heterozygosity were 0.07–0.85 and 0.1–0.88, respectively. Allele numbers ranged from five to 16 per locus.

Evidence for recent population bottlenecks in northern spotted owls (Strix occidentalis caurina)

“…All owls were genotyped at 10 variable microsatellite loci (Table 2) developed for Mexican spotted owls (S. o. lucida; loci: 6H8, 15A6, 13D8, 4E10.2; Thode et al 2002), Lanyu scops owls (Otus elegans botelensis; loci: Oe3-7, Oe053, Oe128, Oe129, Oe149; Hsu et al 2003Hsu et al , 2006, and ferruginous pygmy-owls (Glaucidium brasilianum; locus: FEPO5; Proudfoot et al 2005). One of these loci (Oe128) and an additional microsatellite marker (Bb126) are diagnostic of spotted versus barred owls (Funk et al 2007) and were genotyped to assure that no barred owls or spotted owlbarred owl hybrids were included.…”

Microsatellite loci for distinguishing spotted owls (Strix occidentalis), barred owls (Strix varia), and their hybrids

“…2004), burrowing owls ( Athene cunicularia ; seven; Korfanta et al . 2002), and ferruginous pygmy owls ( Glaucidium brasilianum ; seven; Proudfoot et al . 2005).…”

Variation in DNA microsatellites of the ferruginous pygmy-owl (Glaucidium brasilianum)

“…Ten polymorphic microsatellite loci designed for pygmy-owls (Proudfoot et al 2005) and one locus (4E10.2) from the Mexican spotted owl (Thode et al 2002) were used to derive a genotype for each individual and to assess genetic variation within and between all populations. The 5¢-end of each forward primer was labeled with a fluorescent marker for genotyping on an ABI PRISM 377 automated DNA sequencer (Applied Biosystems, Foster City, California).…”

“…The 5¢-end of each forward primer was labeled with a fluorescent marker for genotyping on an ABI PRISM 377 automated DNA sequencer (Applied Biosystems, Foster City, California). PCR protocols used are described in Proudfoot et al (2005). PCR products were diluted in a mix of formamide and ROX 400 size standard, and genotypes were determined using Genotyper 2.5 (Applied Biosystems).…”

“…In this study, we examine patterns of genetic variation at 11 polymorphic microsatellite loci, 10 of which were isolated from the pygmy-owl (Proudfoot et al 2005) and one from the Mexican spotted owl (Strix occidentalis lucida, Thode et al 2002). Microsatellite markers exhibit high levels of genetic variation and have proven useful for detailed genetic studies within avian species (Barrowclough and Gutie´rrez 1990;Larson et al 2002;Pe´rez et al 2002;Csiki et al 2003).…”