Development and characterization of monoclonal antibodies to spring viraemia of carp virus

Chen, Zhongyuan; Li, Hong; Li, Zhengqiu; Zhang, Qi-Ya

doi:10.1016/j.vetimm.2008.02.011

Cited by 44 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the indirect immunofluorescence assay and ELISA appear to crossreact with other rhabdoviruses (Way, 1991), leading to possible false-positive diagnoses. mAbs are also useful tools for detecting SVCV and studying the function of virus-specific proteins (Chen et al, 2008;Luo et al, 2014;Li et al, 2015). However, because the production of mAbs by traditional hybridoma technology is complicated and requires special skills, alternative methods which are simple and rapid for the production of mAbs are needed.…”

Section: U Ashraf and Othersmentioning

confidence: 99%

Spring viraemia of carp virus: recent advances

Ashraf

Lin

et al. 2016

Journal of General Virology

182

View full text Add to dashboard Cite

Section: U Ashraf and Othersmentioning

confidence: 99%

Spring viraemia of carp virus: recent advances

Ashraf

Lin

et al. 2016

Journal of General Virology

182

View full text Add to dashboard Cite

“…The results were representative of three independent experiments. SVCV was propagated in EPC cells using methods reported previously (44).…”

Section: Antiviral Effect Evaluationmentioning

confidence: 99%

IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish

Hong

et al. 2014

The Journal of Immunology

View full text Add to dashboard Cite

IFN regulatory factor (IRF) 10 belongs to the IRF family and exists exclusively in birds and fish. Most IRFs have been identified as critical regulators in the IFN responses in both fish and mammals; however, the role of IRF10 is unclear. In this study, we identified IRF10 in zebrafish (Danio rerio) and found that it serves as a negative regulator to balance the innate antiviral immune responses. Zebrafish IRF10 (DrIRF10) was induced by intracellular polyinosinic:polycytidylic acid in ZF4 (zebrafish embryo fibroblast-like) cells. DrIRF10 inhibited the activation of zebrafish IFN1 (DrIFN1) and DrIFN3 promoters in epithelioma papulosum cyprinid cells in the presence or absence of polyinosinic:polycytidylic acid stimulation through direct interaction with the IFN promoters, and this inhibition was also shown to block IFN signaling. Overexpression of DrIRF10 was able to abolish the induction of DrIFN1 and DrIFN3 mediated by the retinoic acid–inducible gene I–like receptors. In addition, functional domain analysis of DrIRF10 showed that either the DNA binding domain or the IRF association domain is sufficient for its inhibitory activity for IFN signaling. Lastly, overexpression of DrIRF10 decreased the transcription level of several IFN-stimulated genes, resulting in the susceptibility of host cells to spring viremia of carp virus infection. Collectively, these data suggest that DrIRF10 inhibits the expression of DrIFN1 and DrIFN3 to avoid an excessive immune response, a unique regulation mechanism of the IFN responses in lower vertebrates.

show abstract

“…Cell cultures and virus propagation were performed as described previously (Zhang et al 2001;Chen et al 2008). The initial titer of RGV was 10 5.5 TCID 50 ml -1 , and that of SVCV was 10 5.0 TCID 50 ml -1 .…”

Section: Chromosome Analysismentioning

confidence: 99%

“…Immunofluorescence microscopy examination was done as previously described (Chen et al 2008). In brief, the RGS cells were seeded onto glass coverslips in 6-well plates and fixed by paraformaldehyde at 36 h post-infection by RGV.…”

Section: Immunofluorescence Microscopymentioning

confidence: 99%

Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)

Chen

et al. 2012

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

A red-spotted grouper Epinephelus akaara skin (RGS) cell line was established and characterized. RGS cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25°C. Susceptibilities of RGS and grass carp ovary (GCO) cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS cells was 10 3.5 TCID 50 ml -1 , which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the cells (10 0.5 TCID 50 ml -1 ). The titers of RGV and SVCV in GCO were 10 6.0 TCID 50 ml -1 and 10 8.0 TCID 50 ml -1 , respectively, which were higher than those in RGS cells. The data may imply that RGS cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS cells showed that RGV could replicate in RGS cells. Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection.

show abstract

Development and characterization of monoclonal antibodies to spring viraemia of carp virus

Cited by 44 publications

References 39 publications

Spring viraemia of carp virus: recent advances

Spring viraemia of carp virus: recent advances

IFN Regulatory Factor 10 Is a Negative Regulator of the IFN Responses in Fish

Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)

Contact Info

Product

Resources

About