Development and characterization of polymorphic microsatellite markers in Momordica charantia (Cucurbitaceae)

Abstract

These markers will be useful to study population ecology and population differentiation among M. charantia species and its related species.

Genome-Wide Analysis of Simple Sequence Repeats in Bitter Gourd (Momordica charantia)

“…Most SSRs developed by FIASCO (Wang et al, 2010) and from SSR-enriched fragments (Ji et al, 2012) showed motifs that were consistent with our results. Inconsistencies between our markers and those generated in previous studies may be owing to the differences among individual genomes.…”

“…The genome-based SSR development is far more than the efficiency of traditional methods, including FIASCO (Wang et al, 2010; Guo et al, 2012) and SSR-enriched fragments or libraries (Ji et al, 2012; Saxena et al, 2015). Most SSRs developed by FIASCO (Wang et al, 2010) and from SSR-enriched fragments (Ji et al, 2012) showed motifs that were consistent with our results.…”

“…To validate the predicted motifs, we selected 27 primer pairs for SSR markers developed in two previous studies (Wang et al, 2010; Ji et al, 2012) and aligned these primers to the ‘Dali-11’ reference genome. To further validate the amplification of the unique set of SSR primers developed in this study, 50 primer pairs were randomly selected from 50 unassembled scaffolds (Supplementary Table S3).…”

“…However, since the whole genome sequence was previously unavailable, the development of large-scale SSR markers for bitter gourd has been limited. Only a small proportion of all potential bitter gourd SSR markers, including 26 developed using the fast isolation by AFLP of sequence-containing repeats (FIASCO) method (Wang et al, 2010; Guo et al, 2012), 171 developed from SSR-enriched fragments or libraries (Ji et al, 2012; Saxena et al, 2015), and 50 derived from transcriptomic data (Dhillon et al, 2016), have been reported publicly. This limitation has hindered the efficient genetic improvement of bitter gourd varieties via molecular marker assisted (MAS) approaches as well as many other types of research on the species.…”

Isolation and Characterization of Novel Microsatellite Markers in Chayote [<i>Sechium edule</i> (Jacq.) Sw.]

“…In the present study, 9 out of 10 newly designed microsatellite markers also detected low level of heterozygosity (Ho = 0.00 to 0.20), except for Sed 01 (Ho = 0.85). Other genetic diversity studies on Cucurbitaceae using microsatellite markers demonstrated moderate to high heterozygosity (0.49 -0.75 [28], 0.26 -0.79 [29], 0.00 -1.00 [30], and 0.325 -0.867 [31]). Although individual species in the Cucurbitaceae family are generally cross-pollinated, chayote is known to be self-compatible [32] and a single plant of chayote shows a good fruit set and does not demonstrate clear inbreeding symptoms [33].…”