Isolation and characterization of microsatellite loci for Prunus mongolica (Rosaceae)

Abstract

Premise of the StudyMicrosatellite primers were developed in Prunus mongolica (Rosaceae), a relict flora endemic in arid areas of the Asian interior, to investigate the genetic diversity, phylogeography, population structure, and history of the species.Methods and ResultsFifty‐one microsatellite loci, including di‐, tri‐, and tetranucelotide repeats, were identified using transcriptome sequencing and bioinformatic screening. The number of alleles ranged from seven to 11 and the levels of observed and expected heterozygosity ranged from 0.545 to 1.000 and 0.600 to 0.989, respectively. Most of the primers also amplified in a group of congeneric species (P. triloba, P. davidiana, P. persica, P. cerasifera, and P. serrulata).ConclusionsThis set of microsatellite loci is useful for studying the genetic diversity of P. mongolica. In addition, they can also be used to investigate the population structure, phylogeography, and landscape genetic patterns of congeneric species.

Genetic diversity and population structure of the endangered orchid Pelatantheria scolopendrifolia (Orchidaceae) in Korea

“…Even though microsatellite markers were known to have species-specific characterization, there are many studies that successfully apply microsatellite markers into a closely related species. For example, this has been shown in Pinus resinosa (Pinaceae) [ 59 ], Allium sativum (Lilliaceae) [ 60 ], Artocarpus altilis (Moraceae) [ 61 ], and Prunus mongolica (Rosaceae) [ 62 ]. Therefore, these sets of microsatellite markers will likely prove useful in future population genetic studies related to the genus Pelatantheria as well as P .…”