Development and characterization of ten new microsatellite markers in a mangrove tree species <i>Bruguiera gymnorrhiza</i> (L.)

Islam, Md. Sajedul; Chen, Lian; Kameyama, Norikazu; Wu, Bingyun; Hogetsu, Taizo

doi:10.1111/j.1471-8286.2005.01127.x

Cited by 33 publications

(28 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations, combined with its observed low frequency in the field, were taken as evidence of its likely hybrid origin. Subsequent genetic characterization studies affirmed its status as a distinct hybrid entity (Ge 2001, Ge et al 2004, Pan et al 2005, Islam et al 2006, Zhou et al 2008.…”

mentioning

confidence: 99%

Bruguiera (Rhizophoraceae) in the Indo-West Pacific: a morphometric assessment of hybridization within single-flowered taxa

Duke¹,

Ge²

2011

blum - j plant tax and plant geog

View full text Add to dashboard Cite

mentioning

confidence: 99%

Bruguiera (Rhizophoraceae) in the Indo-West Pacific: a morphometric assessment of hybridization within single-flowered taxa

Duke¹,

Ge²

2011

blum - j plant tax and plant geog

View full text Add to dashboard Cite

“…For the three loci that significantly deviate from the Hardy-Weinberg expectation, the deviation is a heterozygote deficit, again consistent with inbreeding and drift in a small population. It is also possible that some of the deviation from Hardy-Weinberg expectations may result from the presence of null alleles for these loci (Islam et al 2006). Inbreeding and self fertilization have been previously reported in mangrove species (Maguire et al 2000;Chen et al 1996;Nunez-Farfan et al 2002), so this may prove to be an adaptation of mangroves for colonizing new habitats.…”

Section: Discussionmentioning

confidence: 93%

“…Ten microsatellite regions previously designed for B. gymnorrhiza (Islam et al 2006) were amplified via the polymerase chain reaction. PCR reactions were carried out in 25 ll volumes with *50 ng genomic DNA, 2 mM MgCl 2 , each dNTP at 10 mM, 19 Promega GoTaq flexi buffer B, 0.75 units Promega Taq, and 1 lmole of each primer.…”

Section: Molecular Genetic Analysis Of B Gymnorrhiza From the Kampongmentioning

confidence: 99%

Are mangroves in the tropical Atlantic ripe for invasion? Exotic mangrove trees in the forests of South Florida

et al. 2009

View full text Add to dashboard Cite

Two species of mangrove trees of IndoPacific origin have naturalized in tropical Atlantic mangrove forests in South Florida after they were planted and nurtured in botanic gardens. Two Bruguiera gymnorrhiza trees that were planted in the intertidal zone in 1940 have given rise to a population of at least 86 trees growing interspersed with native mangrove species Rhizophora mangle, Avicennia germinans and Laguncularia racemosa along 100 m of shoreline; the population is expanding at a rate of 5.6% year -1 .Molecular genetic analyses confirm very low genetic diversity, as expected from a population founded by two individuals. The maximum number of alleles at any locus was three, and we measured reduced heterozygosity compared to native-range populations. Lumnitzera racemosa was introduced multiple times during the 1960s and 1970s, it has spread rapidly into a forest composed of native R. mangle, A. germinans, Laguncularia racemosa and Conocarpus erectus and now occupies 60,500 m 2 of mangrove forest with stem densities of 24,735 ha -1 . We estimate the population growth rate of Lumnitzera racemosa to be between 17 and 23% year -1 . Populations of both species of naturalized mangroves are dominated by young individuals. Given the long life and water-dispersed nature of propagules of the two exotic species, it is likely that they have spread beyond our survey area. We argue that the species-depauperate nature of tropical Atlantic mangrove forests and close taxonomic relatives in the more species-rich Indo-Pacific region result in the susceptibility of tropical Atlantic mangrove forests to invasion by Indo-Pacific mangrove species.

show abstract

“…The PCR cycling conditions were as follows: 1 min at 94°C, followed by 29 cycles of 30 s at 94°C, 1 min at the annealing temperature for the primer pair, and 1 min at 72°C, followed by one cycle of 30 s at 94°C, 1 min at the annealing temperature for the primer pair, and 5 min at 72°C. To analyze the polymorphisms in the cpSSR loci of B. gymnorrhiza and K. candel, we employed the tailed primer method as reported by Schuelke (2000) and Islam et al (2006). In this method, fluorescence-labeled cpSSR marker primers are substituted with non-labeled primers tailed with the U19 sequence (5 0 -GGTTTTCCCAGTCACG ACG-3 0 ) at their 5 0 ends.…”

mentioning

confidence: 99%

Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species

et al. 2007

View full text Add to dashboard Cite

Chloroplast microsatellite (cpSSR) markers were developed for three ecologically and economically important tree species in the mangrove family, Rhizophoraceae: Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa. Noncoding regions of chloroplast DNA (cpDNA) from each species were separately amplified using universal chloroplast primers. Six, two, and three polymorphic cpSSR loci in B. gymnorrhiza, K. candel, and R. stylosa, respectively, were developed from amplified noncoding cpDNA regions. Characterization of 216, 156, and 253 individuals of B. gymnorrhiza, K. candel, and R. stylosa, respectively, collected from different natural mangrove populations (B. gymnorrhiza, 9; K. candel, 7; R. stylosa, 9) on Iriomote Island in Japan showed that these loci provide cpSSR markers with polymorphisms ranging from two to four alleles per locus and gene diversity between 0.027 and 0.480. These cpSSR markers will be useful for analyzing the maternal lineage distributions and population genetic structures of the three species. Several of these markers may also be useful in similar studies of other mangrove species.Keywords cpSSR Á Mangrove Á Maternal lineage Á Rhizophoraceae Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa are ecologically and economically important tree species of a widely distributed mangrove family, the Rhizophoraceae. Ecologically, these three species serve as important components of the coastal mangrove ecosystem, as they protect shorelines by controlling coastal erosion and reducing the damaging effects of tidal surges and cyclonic storms in the coastal areas, and maintain the food web of coastal marine ecosystems. These species are important economically to communities living adjacent to mangroves, because of their use as timber and fuelwood and as raw materials for cottage industries. Information on population genetics and mating systems can provide effective guidelines for ecological management and conservation of these species. In the present study, chloroplast microsatellite (cpSSR) markers were isolated from B. gymnorrhiza, K. candel, and R. stylosa in order to investigate their maternal lineages, population genetic structures, and mating systems.Genomic DNA was extracted from silica gel-dried leaves using a modified cetyltrimethyl ammonium bromide (CTAB) method . cpSSR regions were isolated from B. gymnorrhiza, K. candel, and R. stylosa by sequencing the noncoding regions of chloroplast DNA (cpDNA) following the method of Lian et al. (2003). Briefly, the cpDNA fragments of each species were amplified by PCR using 11 universal chloroplast primer pairs developed by Taberlet et al. (1991), Demesure et al.

show abstract

Development and characterization of ten new microsatellite markers in a mangrove tree species Bruguiera gymnorrhiza (L.)

Cited by 33 publications

References 15 publications

Bruguiera (Rhizophoraceae) in the Indo-West Pacific: a morphometric assessment of hybridization within single-flowered taxa

Bruguiera (Rhizophoraceae) in the Indo-West Pacific: a morphometric assessment of hybridization within single-flowered taxa

Are mangroves in the tropical Atlantic ripe for invasion? Exotic mangrove trees in the forests of South Florida

Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species

Contact Info

Product

Resources

About