Development and demonstration of a cleaner process to produce diosgenin from Dioscorea zingiberensis based on physical separation

Pan, Cenxuan; Zhao, Yangsheng; Liu, Guihua; Dou, Guangyu; Ru, Zhen-guang; Zhu, Kesong

doi:10.1016/j.jclepro.2013.12.074

Cited by 12 publications

(2 citation statements)

References 14 publications

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“…Metode ekstraksi yang dipakai menggunakan campuran enzim dan ultrafiltrasi agar diperoleh kadar diosgenin optimal. Kelebihan metode tersebut dibanding metode konvensional ialah mengurangi penggunaan bahan kimia sehingga mengurangi pencemaran air (Liu et al 2010;Zhu et al 2010;Wei et al 2013;Pan et al 2014).…”

Section: Kuantifikasi Diosgenin Dengan Spektrofotometri Uv-visunclassified

Karakterisasi Kuantitatif Diosgenin dengan Spetrofotometri UV-Vis pada Koleksi Umbi Dioscorea spp. di Indonesia

Haryati¹,

Dwiatmini²,

Diantina³

et al. 2020

BPN

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<p>Dioscorea spp. merupakan tanaman umbi-umbian yang termasuk dalam kategori pangan fungsional karena mengandung senyawa bioaktif, di antaranya ialah diosgenin. Senyawa ini merupakan senyawa golongan steroid saponin digunakan sebagai bahan baku dalam sintesis obat golongan steroid. Sumber utama senyawa diosgenin adalah Dioscorea spp. Indonesia sebagai salah satu center of origin Dioscorea berpeluang untuk menjadi produsen diosgenin dunia karena mempunyai beragam plasma nutfah Dioscorea. Oleh sebab itu, perlu dikaji potensi diosgenin dari Dioscorea spp. lokal Indonesia. Dalam penelitian ini dilakukan karakterisasi kuantitatif senyawa diosgenin menggunakan metode spektrofotometri Ultra Violet-Visible (UV-Vis) pada 26 aksesi Dioscorea spp. lokal Indonesia, terdiri atas 10 aksesi D. alata, 7 aksesi D. esculenta, dan 9 aksesi D. hispida. Validasi metode spektrofotometri UV-Vis ditentukan berdasarkan nilai korelasi linear, LOD, dan LOQ. Ekstraksi diosgenin dilakukan dengan refluks dan<br />hidrolisis pada saat yang bersamaan dengan pelarut isopropanol 70% dan H2SO4 20%. Metode UV-Vis yang digunakan dalam penelitian ini cukup sensitif dan akurat dengan nilai korelasi linear 0,996, LOD 2,604 μg, dan LOQ 7,890 μg. Kandungan diosgenin tertinggi diperoleh dari aksesi D. hispida dengan kode II sebesar 0,294%. D. hispida dapat dimanfaatkan sebagai kandidat sumber potensial senyawa diosgenin.</p>

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Section: Kuantifikasi Diosgenin Dengan Spektrofotometri Uv-visunclassified

Karakterisasi Kuantitatif Diosgenin dengan Spetrofotometri UV-Vis pada Koleksi Umbi Dioscorea spp. di Indonesia

Haryati¹,

Dwiatmini²,

Diantina³

et al. 2020

BPN

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“…Though pretreatment of DZW tubers could promote the extraction of diosgenin effectively (Mi et al, 2016;Pan et al, 2014;Wang et al, 2008). The disogenin production still performed based on acid hydrolysis, which could not meet the demand of environmental protection.…”

Section: Optimization Of the Fermentation Conditions Through Responsementioning

confidence: 99%

A cleaner production of diosgenin from Dioscorea Zingiberensis Wright biotransformed by Lactobacillus casei

2017

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The effect of 24 probiotics on disogenin production had been conducted in our preliminary work. Consequently, Lactobacillus casei was selected as the fermentation strain with high potential in biotransformation and effects of main parameters on biotransformation were verified through single factor variable experiments. The fermentation conditions were optimized using response surface methodology, which would be helpful to enhance biotransformation efficiency and achieve a high-valued utilization of DZW in diosgenin production. Single factor variable experiments indicated that biotransformation was highly depend on temperature. The conversion rate of saponin reached to (22.1 ± 0.6) %, (15.5 ± 0.3) % and (17.0 ± 0.4) % under each optimal condition of 43°C, 24 hours and 4% inoculum size respectively. According to results of single factor variable experiments, fermentation conditions were optimized through response surface methodology. The 32.4% of conversion rate and 3.95 µg/mL of diosgenin concentration were predicted to be optimal value under the condition of 42°C, 27 hours, 3% inoculum size. The verification results were (32.8 ± 0.9)% of conversion rate and (4.01±0.02) µg/mL of diosgenin concentration under the same conditions, which were close to the predicted values. The conversion rate increased by 41.9% compared to the initial value. Feasibility of converting saponin to diosgenin through probiotics by biotransformation was verified in this paper and the results could be the reference for a cleaner production of diosgenin.

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Identification and a phased pH control strategy of diosgenin bio-synthesized by an endogenous Bacillus licheniformis Syt1 derived from Dioscorea zingiberensis C. H. Wright

Wang

Pan

et al. 2021

Appl Microbiol Biotechnol

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Development and demonstration of a cleaner process to produce diosgenin from Dioscorea zingiberensis based on physical separation

Cited by 12 publications

References 14 publications

Karakterisasi Kuantitatif Diosgenin dengan Spetrofotometri UV-Vis pada Koleksi Umbi Dioscorea spp. di Indonesia

Karakterisasi Kuantitatif Diosgenin dengan Spetrofotometri UV-Vis pada Koleksi Umbi Dioscorea spp. di Indonesia

A cleaner production of diosgenin from Dioscorea Zingiberensis Wright biotransformed by Lactobacillus casei

Identification and a phased pH control strategy of diosgenin bio-synthesized by an endogenous Bacillus licheniformis Syt1 derived from Dioscorea zingiberensis C. H. Wright

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