Development and differentiation of the erythroid lineage in mammals

Barminko, Jeffrey; Reinholt, Brad M.; Baron, Margaret H.

doi:10.1016/j.dci.2015.12.012

Cited by 43 publications

(30 citation statements)

References 192 publications

(270 reference statements)

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“…Developmental studies using chicken and Xenopus embryos, and the in vitro differentiation of mouse (Chung et al, 2002) and human (Choi et al, 2012) embryonic stem cells and induced-pluripotent cells, indicate that endothelial and blood cells emerge from a common cellular ancestor, the hemangioblast, that has dual vascular and hematopoietic potential (Choi et al, 1998;Faloon et al, 2000;Lancrin et al, 2009). During mammalian embryogenesis, yolk sacderived hematopoietic precursors generate embryonic or 'primitive' erythroid cells and macrophages (Barminko et al, 2016). In the mouse, primitive hematopoiesis is followed by a blood-producing process involving an 'endothelial to hematopoietic transition'.…”

Section: An Overview Of the Stem And Progenitor Cell Transitions Thatmentioning

confidence: 99%

Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond

et al. 2018

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Hemoglobin-expressing erythrocytes (red blood cells) act as fundamental metabolic regulators by providing oxygen to cells and tissues throughout the body. Whereas the vital requirement for oxygen to support metabolically active cells and tissues is well established, almost nothing is known regarding how erythrocyte development and function impact regeneration. Furthermore, many questions remain unanswered relating to how insults to hematopoietic stem/progenitor cells and erythrocytes can trigger a massive regenerative process termed 'stress erythropoiesis' to produce billions of erythrocytes. Here, we review the cellular and molecular mechanisms governing erythrocyte development and regeneration, and discuss the potential links between these events and other regenerative processes.

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Section: An Overview Of the Stem And Progenitor Cell Transitions Thatmentioning

confidence: 99%

Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond

et al. 2018

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“…Definitive hematopoiesis, however, later shifts to the fetal liver for expansion. Embryonic hematopoiesis can be divided into three stages or waves [89]. The yolk sac exhibits both primitive and definitive hematopoietic activity [2101112].…”

Section: Introductionmentioning

confidence: 99%

Regulation of the embryonic erythropoietic niche: a future perspective

2017

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The production of red blood cells, termed erythropoiesis, occurs in two waves in the developing mouse embryo: first primitive erythropoiesis followed by definitive erythropoiesis. In the mouse embryo, both primitive and definitive erythropoiesis originates in the extra-embryonic yolk sac. The definitive wave then migrates to the fetal liver, fetal spleen and fetal bone marrow as these organs form. The fetal liver serves as the major organ for hematopoietic cell expansion and erythroid maturation after mid-gestation. The erythropoietic niche, which expresses critical cytokines such as stem cell factor (SCF), thrombopoietin (TPO) and the insulin-like growth factors IGF1 and IGF2, supports hematopoietic expansion in the fetal liver. Previously, our group demonstrated that DLK1+ hepatoblasts support fetal liver hematopoiesis through erythropoietin and SCF release as well as extracellular matrix deposition. Loss of DLK1+ hepatoblasts in Map2k4−/− mouse embryos resulted in decreased numbers of hematopoietic cells in fetal liver. Genes encoding proteinases and peptidases were found to be highly expressed in DLK1+ hepatoblasts. Capitalizing on this knowledge, and working on the assumption that these proteinases and peptidases are generating small, potentially biologically active peptides, we assessed a range of peptides for their ability to support erythropoiesis in vitro. We identified KS-13 (PCT/JP2010/067011) as an erythropoietic peptide-a peptide which enhances the production of red blood cells from progenitor cells. Here, we discuss the elements regulating embryonic erythropoiesis with special attention to niche cells, and demonstrate how this knowledge can be applied in the identification of niche-derived peptides with potential therapeutic capability.

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“…Erythropoiesis is a complex, multi-step process coupling cell division with differentiation, starting from a heterogeneous population of haematopoietic stem cells 35 and resulting in mature reticulocytes. 36 Here we demonstrate that this can be modeled in an ex vivo culture system with erythroblasts passing through the expected stages of differentiation with appropriate expression of erythroid cell surface markers.…”

Section: Discussionmentioning

confidence: 99%

Modelling erythropoiesis in congenital dyserythropoietic anaemia type I (CDA-I)

Scott

Downes

Brown

et al. 2019

Preprint

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We employ and extensively characterise an ex vivo culture system to study terminal erythroid maturation of CD34 + progenitors from the peripheral blood of normal individuals and patients with Congenital Dyserythropoietic Anaemia type 1 (CDA-I). Using morphological analysis, FACS analysis and the proteomic approach CyTOF, we analysed patient-derived erythroblasts stage-matched with those from healthy donors during the expansion phase and into early differentiation. In patient cells, aspects of disordered erythropoiesis manifest midway through differentiation, including increased proliferation and changes in the DNA accessibility profile. We also show that cultured erythroblasts from CDA-I patients recapitulate the pathognomic feature of this erythroid disorder with up to 40% of the cells having abnormal 'spongy' chromatin morphology by electron microscopy, as well as upregulation of GDF15, a marker of ineffective erythropoiesis. In the tertiary phase of culture, patient cells show significantly less enucleation and there is persistence of earlier erythroid precursors. Furthermore, the enucleation defect appears to be more severe in patients with mutations in C15orf41, as compared to the other known causative gene CDAN1, indicating a genotype/phenotype correlation in CDA-I. Such erythroblasts are a valuable resource for investigating the pathogenesis of this disease and provide the opportunity for streamlining diagnosis for CDA-I patients and ultimately other forms of unexplained anaemia. Introduction:

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Development and differentiation of the erythroid lineage in mammals

Cited by 43 publications

References 192 publications

Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond

Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond

Regulation of the embryonic erythropoietic niche: a future perspective

Modelling erythropoiesis in congenital dyserythropoietic anaemia type I (CDA-I)

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