Development and economic evaluation of an eco-friendly biocatalytic synthesis of emollient esters

Serrano‐Arnaldos, Mar; Montiel, M.C.; Ortega‐Requena, Salvadora; Máximo, Fuensanta; Bastida, Jaume

doi:10.1007/s00449-019-02243-1

Cited by 16 publications

(34 citation statements)

References 28 publications

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“…The kinetic model previously detailed has been used to describe the enzymatic synthesis of the four main components of spermaceti. For this purpose, the experimental conversion data obtained during the synthesis of the four cetyl esters by varying biocatalyst concentration, 18,19 have been fitted to Eqn (22) using the software CurveExpert Basic 1.40.…”

Section: Resultsmentioning

confidence: 99%

“…This may be explained by the fact that, in this last case, the reaction step may have more influence due to the lower activity of the biocatalyst, which justifies that the optimum amount selected for Lipozyme ® TL IM during the experimental optimization was twice the quantity chosen for the other immobilized enzymes. 18,19 Additionally, the model can be easily checked by comparing the values of the calculated and experimental conversions. As can be seen in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Experimental data for the modelling of the enzymatic synthesis of 20 g cetyl laurate, myristate, palmitate and stearate, or the spermaceti analogue in a solvent-free medium, have been described by the authors in previous works. 18,19 The process was performed in a jacketed Parr 5101 series tank reactor, equipped with an overhead four-bladed impeller, under low pressure and inert wileyonlinelibrary.com/jctb N 2 atmosphere conditions. In order to promote mass transfer while safeguarding the biocatalyst from mechanical breakdown, all the assays were performed at 350 rpm.…”

Section: Experimental Datamentioning

confidence: 99%

“…( immobilized on a cationic silicate. In particular, the results from biocatalyst concentration and process temperature optimization 18,19 were used to validate the kinetic modelling proposed for the lipase-based esterification process. Experimental conversion (X) was calculated based on acid value measurements.…”

Section: Experimental Datamentioning

confidence: 99%

“…From the integration of Eqn (18) with the initial condition shown in Eqn (19), the variation of C A along the time is obtained (Eqn 20): www.soci.org M Serrano-Arnaldos et al…”

Section: A ð9þmentioning

confidence: 99%

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A simplified kinetic model to describe the solvent‐free enzymatic synthesis of wax esters

Serrano‐Arnaldos

Murcia

Ortega‐Requena

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: The use of biotechnological processes at industrial scale is a promising tool to replace conventional synthesis as an efficient and eco-friendly technology. For that purpose, the kinetic modelling of an in-lab optimized enzymatic process prior to scaling-up is of great utility. RESULTS: In this work, a kinetic model for the solvent-free synthesis of cetyl laurate, myristate, palmitate and stearate using different commercial immobilized lipases has been developed. In order to describe the esterification process of the cetyl esters separately and as a mixture similar to natural spermaceti, a pseudo-first-order kinetic has been proposed and tested. A relation between the inverse values of the kinetic constant and the amount of biocatalyst has been observed. The effect of temperature on the reaction rate can be accurately described by the Arrhenius equation except for immobilized Thermomyces lanuginosus, which appears to be less resistant to temperatures above 70°C.CONCLUSION: Low deviations between experimental and predicted values (R 2 ≥ 0.99) indicate that this pseudo-first-order kinetic model can be considered valid for the data range studied. In addition, the reaction rate of spermaceti can be successfully predicted through a weighted average of the kinetic constants obtained during the synthesis of each cetyl ester. This simple but accurate kinetic model for describing the solvent-free enzymatic biosynthesis of wax esters from spermaceti may contribute to extending the application of lipases as industrial catalysts.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Experimental Datamentioning

confidence: 99%

Section: Experimental Datamentioning

confidence: 99%

“…From the integration of Eqn (18) with the initial condition shown in Eqn (19), the variation of C A along the time is obtained (Eqn 20): www.soci.org M Serrano-Arnaldos et al…”

Section: A ð9þmentioning

confidence: 99%

See 3 more Smart Citations

A simplified kinetic model to describe the solvent‐free enzymatic synthesis of wax esters

Serrano‐Arnaldos

Murcia

Ortega‐Requena

et al. 2021

J of Chemical Tech & Biotech

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Enzymatic production of wax esters by esterification using lipase immobilized via physical adsorption on functionalized rice husk silica as biocatalyst

Machado

Sabi

Hirata

et al. 2023

Biotech and App Biochem

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The present study consists of developing an enzymatic process for the production of wax esters (lauryl stearate and cetyl stearate) by esterification in a heptane medium. Lipase from Thermomyces lanuginosus (TLL) immobilized via interfacial activation on silica particles from rice husks functionalized with triethoxy(octyl)silane (TLL–Octyl–SiO2) was used as biocatalyst. Maximum immobilized protein loading of around 22 mg g−1 (that corresponds to an immobilization yield of ≈55%) of support was observed using an initial protein loading of 40 mg g−1 of Octyl–SiO2. Its hydrolytic activity (olive oil emulsion hydrolysis) was of 620 U g−1 of biocatalyst. The effect of certain factors on the cetyl estearate production was evaluated using a central composite rotatable design (CCDR). Under optimal conditions (64°C, 21% of mass of biocatalyst per volume of reaction mixture, 170 rpm, and stoichiometric acid:alcohol molar ratio 1 mol L−1 of each reactant), maximum acid conversion percentage of 91% was observed after 60 min of reaction. Lauryl stearate was also produced under such conditions, and an acid conversion of 93% after 60 min of reaction was also achieved. Free lipase exhibited acid conversion of only 15%–20% for both reaction mixtures. After nine successive esterification batches, TLL–Octyl–SiO2 retained 85%–90% of its original activity. These results show the promising use of the prepared biocatalyst in wax esters production due to its high catalytic activity and reusability.

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Development of an industrial sustainable process for wax esters production: enzyme immobilization, process optimization, and plant simulation

Montiel

Serrano‐Arnaldos

Yagüe

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND: As a result of the ban on whaling, there has been a shortage of spermaceti, a natural product with applications in cosmetics and pharmaceuticals. A green and sustainable process for the biocatalytic synthesis of a wax ester mixture analogue to natural spermaceti is presented in this paper.RESULTS: Immobilization of Candida antarctica lipase B by covalent binding to Purolite® Lifetech™ ECR8285 allowed for an immobilized derivative with 124.5 mg protein/g support (69.3% protein immobilization yield) that maintains 100% of its enzymatic activity after 12 months of cold storage and presents negligible loses of activity after 9 consecutive reaction cycles. The optimization of the synthesis process in a batch reactor resulted in conditions that, at 70 °C, 350 rpm, and 1.25% w/w of biocatalyst, achieved a conversion of 97% after 1 h of reaction. The simulation of a spermaceti production plant was carried out using the process simulation software aspenONE suite v10. The plant was designed for a continuous operation during 9 h per day, with a reactor of 20.25 L of working volume and a residence time of 1 h. The production of this plant would be 173.25 kg spermaceti/day, with a product purity of 99.55%. CONCLUSION:The main novelty of this work is the design of a spermaceti production plant, using the most sustainable methodologies and resulting in a product with exceptional characteristics and minimal waste generation. Moreover, a new lipase immobilized derivative is also described. The good values of sustainability indicators point to the viability of its industrial implementation.

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Development and economic evaluation of an eco-friendly biocatalytic synthesis of emollient esters

Cited by 16 publications

References 28 publications

A simplified kinetic model to describe the solvent‐free enzymatic synthesis of wax esters

A simplified kinetic model to describe the solvent‐free enzymatic synthesis of wax esters

Enzymatic production of wax esters by esterification using lipase immobilized via physical adsorption on functionalized rice husk silica as biocatalyst

Development of an industrial sustainable process for wax esters production: enzyme immobilization, process optimization, and plant simulation

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