Development and Evaluation of a Loop-mediated Isothermal Amplification Combined with Au-nanoprobe Assay for Rapid Detection of Mycobacterium tuberculosis

Kaewphinit, Thongchai; Ckumdee, Jutturong; Chansiri, Kosum; Santiwatanakul, Somchai

doi:10.4103/ijmm.ijmm_15_333

Cited by 7 publications

(8 citation statements)

References 5 publications

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“…The search was done in December 2020, and we obtained 1,008 hints from four journal databases. We selected three experimental studies that best answer the clinical question; a study from Thailand which explains the development of LAMP with the addition of Au-NP as a novel strategy in diagnosing TB, and two studies (from Portugal and Thailand) that use LAMP with Au-Np to detect rifampicin and isoniazid resistance [12,13,15]. A flowchart of the selected article process can be seen in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

“…Notomi et al [14] first introduced a method to amplify DNA in an isothermic process called Loop-Mediated Isothermal Amplification (LAMP) in 2000. The method boasts simplicity and efficiency compared to PCR by requiring only a constant temperature of around 60-65 °C and has a comparable diagnostic accuracy, if not superior, to smear microscopy [12,15,81]. This method's high specificity is owed to four primers' DNA recognition at the start of the amplification and two at the next stage [14].…”

Section: Loop-mediated Isothermal Amplification and The Working Principlementioning

confidence: 99%

“…A study by Kaewphinit et al [15] compares LAMP and Au-Np with culture as a gold standard and acid-fast bacilli (AFB) smear test as a reference to evaluate the ability to detect M. tuberculosis infection. They found that the sensitivity was 98.2% in raw sputum samples but only 55.6% in culture-positive/smear-negative samples, while specificity was found to be 88.2% [15]. The study, however, did not evaluate its ability to detect antibiotic resistance.…”

Section: Effectivity Of Lamp and Au-np In The Detection Of Isoniazid And Rifampicin Resistancementioning

confidence: 99%

“…In exploring the advantages and applications, although studies on LAMP and Au-Np combination are scarce, the research by Kaewphinit et al [15] has at least shown that LAMP and Au-Np combination can be implemented for rapid detection of TB in a developing country, in this case, Thailand. On the other hand, studies using LAMP without Au-Np as a diagnostic tool are abundant and show the applicability of LAMP in different countries of different conditions.…”

Section: Crs and Culture As Referencementioning

confidence: 99%

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Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Habiburrahman

Ariq

Handayani

2021

J Infect Dev Ctries

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Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics. However, current efforts to detect resistances face challenges such as limited equipment, budget, and time. This evidence-based review investigated loop-mediated isothermal amplification, an alternative molecular diagnostic tool with promising performance and applicability in developing countries, and its use combined with Au-Nanoprobe to detect antibiotic resistance in tuberculosis. The literature search was conducted through four databases (Proquest, EBSCOhost, Scopus, and Pubmed) for useful articles on loop-mediated isothermal amplification and Au-Nanoprobe in detecting tuberculosis and tuberculosis resistance. After filtering the result with inclusion and exclusion criteria, the search produced three papers that best answer the clinical question. Loop-mediated isothermal amplification amplifies a target sequence, and Au-Nanoprobe responds to the DNA specific to the target mutant, producing an observable color change. Loop-mediated isothermal amplification and Au-Nanoprobe showed 100% sensitivity and specificity in detecting rifampicin and isoniazid resistance. Another study investigated its viability to detect tuberculosis and found 98.2% sensitivity and 88.2% specificity. Combining loop-mediated isothermal amplification and Au-Nanoprobe had a shorter time to get results and should also be relatively cheaper because it does not need a high temperature to work and requires less equipment. In conclusion, loop-mediated isothermal amplification and Au-Nanoprobe can be used as an efficient and accurate method to detect isoniazid and rifampicin-resistant tuberculosis strains. The new technology is promising for developing countries due to their high disease burden but facing several healthcare barriers.

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Section: Methodsmentioning

confidence: 99%

Section: Loop-mediated Isothermal Amplification and The Working Principlementioning

confidence: 99%

Section: Effectivity Of Lamp and Au-np In The Detection Of Isoniazid And Rifampicin Resistancementioning

confidence: 99%

Section: Crs and Culture As Referencementioning

confidence: 99%

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Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Habiburrahman

Ariq

Handayani

2021

J Infect Dev Ctries

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“…In a novel nanodiagnostic strategy, the LAMP-AuNP probe method, showed no cross reaction with other mycobacteria and offered a short total assay time of <1 h. Scientists have proved that this assay is highly sensitivity, takes relatively shorter analysis time, cost effective and has lack of requirement for a thermocycler and separate detection reagents. Therefore, the LAMP-AuNP MTB probe assay constitutes a safe and simple alternative to traditional PCR for the rapid and sensitive detection of MTB DNA (Kaewphinit et al 2017).…”

Section: B Based On Modified Nanoprobesmentioning

confidence: 99%

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

Bharti¹,

Verma²,

Dubey³

et al. 2021

IJAR

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Tuberculosis (TB) is an infectious and contagious disease caused by various strains of Mycobacterium and the foremost leading cause of death of animal and human population worldwide. Currently available conventional and modern diagnostic techniques are useful but they are neither rapid nor cost effective for early and accurate diagnosis, represents a cornerstone to eradicate TB worldwide by 2030. To overcome this awful situation an innovative strategy i.e. nanotechnology is helpful for early diagnosis of tuberculosis in short time with increased sensitivity and specificity through nano sized biomolecular interaction viz. enhanced visualization of fluorescent signals, visual colorimetric signals of amplified DNA product, nanofluidic technology, nuclear magnetic resonance technology (NMR) and prototype miniaturized device etc. Nanotechnology has introduced new paradigms for molecular diagnostics. However, some constraints of nanodiagnostic tool still there that make obstacle in its worldwide diagnostic use for TB.

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An inexpensive and rapid diagnostic method for detection of SARS-CoV-2 RNA by loop-mediated isothermal amplification (LAMP)

Prakash¹,

Priyatma²,

Aasarey³

et al. 2023

MethodsX

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Development and Evaluation of a Loop-mediated Isothermal Amplification Combined with Au-nanoprobe Assay for Rapid Detection of Mycobacterium tuberculosis

Cited by 7 publications

References 5 publications

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Prospects and Application of Nanotechnology for Diagnosis of Tuberculosis in Livestock: A Review

An inexpensive and rapid diagnostic method for detection of SARS-CoV-2 RNA by loop-mediated isothermal amplification (LAMP)

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