Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus

Jiang, Lixiang; Luo, Jing; Wu, Dong; Wang, Chengmin; Jin, Wen; Xia, Yuetong; Wang, Haijing; Ding, Hua; Jiang, Long; He, Hongxuan

doi:10.1016/j.jviromet.2015.03.013

Cited by 58 publications

(37 citation statements)

References 48 publications

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“…PDA was prepared from PCDA and DMPC as previously described by Jiang and co‐workers . Briefly, PCDA and DMPC (6: 4) were dissolved in chloroform and evaporated at 50°C.…”

Section: Methodsmentioning

confidence: 99%

Exosome aggregation mediated stop‐flow paper‐based portable device for rapid exosome quantification

et al. 2020

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Exosome quantification is important for estimation of informative messengers (e.g., proteins, lipids, RNA, etc.) involving physiological and pathological effects. This work aimed to develop a simple and rapid distance‐based paper portable device using exosome‐capture vesicles (polydiacetylene conjugated with antiCD81) for exosome quantification in cell cultures. This novel concept relied on distinct aggregation of exosomes and exosome‐capture vesicles leading to different solvent migration. Distances of the migration were used as signal readouts, which could be detected by naked eye. PDA‐antiCD81 as exosome‐capture vesicles were optimized (e.g., size, reaction ratio, and concentration) and the paper designs were investigated (e.g., diameter of sample reservoir and lamination layer) to enhance the solvent stop‐flow effects. Finally, exosome screening on three cell culture samples (COLO1, MDA‐MB‐231, and HuR‐KO1 subclone) was demonstrated. The method could linearly measure exosome concentrations in correlation with solvent migration distances in the range of 106–1010 particles/mL (R2 > 0.98) from the cell culture samples. The exosome concentration measurements by the developed device were independently assessed by nanoparticle tracking analysis. Results demonstrated no statistically significant difference (p > 0.05) by t‐test. This low‐cost and rapid device allows a portable platform for exosome quantification without the requirement of expensive equipment and expertise of operation. The developed device could potentially be useful for quantification of other biomarker‐related extracellular vesicles.

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“…PDA was prepared from PCDA and DMPC as previously described by Jiang and co‐workers . Briefly, PCDA and DMPC (6: 4) were dissolved in chloroform and evaporated at 50°C.…”

Section: Methodsmentioning

confidence: 99%

Exosome aggregation mediated stop‐flow paper‐based portable device for rapid exosome quantification

et al. 2020

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“…PDA vesicles are fabricated using 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) that form a lipid solution in chloroform, which is then rotoevaporated, sonicated, cooled and stored at 4°C for crystallization of lipid membranes, then UV irradiated at 254 nm for 5 min to attain blue PDA solution [156,158]. For example the H5N1 virus is detected with high specificity using PDA by causing the color of the assay to change from blue to red [158]. The sensitivity of the bioassay is 0.53 copy per lL, which is comparable to RT-PCR.…”

Section: Polymerized Polydiacetylenementioning

confidence: 99%

Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

102

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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“…[ 56 , 57 , 58 ]: sialic acid was grafted on β-glucoside and sialic and lactose moieties were grafted on a glucoside chain for insertion in the PDA layer. Different other types of virus receptor were grafted onto PDA moieties: antibodies [ 59 , 60 ] and peptides [ 61 ].…”

Section: Pda Vesicle Based Assays For Detection Of Influenza Virusmentioning

confidence: 99%

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Lebègue

Farre

Jose

et al. 2018

Sensors

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Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed.

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Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus

Cited by 58 publications

References 48 publications

Exosome aggregation mediated stop‐flow paper‐based portable device for rapid exosome quantification

Exosome aggregation mediated stop‐flow paper‐based portable device for rapid exosome quantification

Colorimetric biosensors for point-of-care virus detections

Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

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