Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid serotyping of foot-and-mouth disease virus

Madhanmohan, M.; Singanallur, Nagendrakumar Balasubramanian; Manikumar, Kankipati; Sambandam, Yuvaraj; Parida, Satya; Srinivasan, V.A.

doi:10.1016/j.jviromet.2012.08.015

Cited by 28 publications

(39 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, RT-LAMP was found suitable for FMDV serotyping at 63 °C for 60 min; this finding was also reported by Madhanmohan et al (9). In our study, the dilution), whereas, in an earlier study, Madhanmohan et al (9) reported it to be 1000 (10 -3 dilution) for serotype O and Asia-1 and 10,000 (10 -4 dilution) of serotype A. Moreover, the detection limit in our study for RT-LAMP and rRT-PCR (TaqMan) was up to 100,000 (10 -5 dilution) of FMDV serotypes A, O, and Asia-1 and this appears 100% comparable with TaqMan real-time PCR.…”

Section: Discussioncontrasting

confidence: 56%

“…Furthermore, the specificity of RT-LAMP was 100% with no cross-reactivity and this was also observed by Madhanmohan et al (9). In contrast to this, Dukes et al (14) evaluated RT-LAMP assay with TaqMan rRT-PCR and reported 81/98 (82.65%) and 94/98 (95.91%) positive by RT-LAMP and rRT-PCR, respectively.…”

Section: Discussionmentioning

confidence: 54%

“…It was further evaluated using field samples in Pakistan. FMDV 3D specific primers (8) and FMDV VP1 specific primers (9) were used in the RT-LAMP for amplification of FMDV RNA. Sequence details of 3D specific and VP1 specific primers are given in Tables 1 and 2, respectively.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Maryam¹,

Rasheed²,

Latif³

et al. 2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

Availability of a rapid and sensitive diagnostic technique is key in successful prevention and control of infectious diseases like foot-and-mouth disease (FMD). Existing conventional diagnostic tests for FMD are laborious and time-consuming with low sensitivity and specificity. Molecular-based techniques are costly and difficult, involving refined apparatus like a thermal cycler. In the present study, the technique of real-time loop-mediated isothermal amplification (RT-LAMP) was standardized for diagnosing FMDV and its serotypes, evaluated using field samples, and compared with the existing real-time PCR in Pakistan. RT-LAMP amplified the target 3D gene using specific primers at 65 °C for 60 min and the VP1 gene using serotype specific primers at 63 °C for 60 min. A total of 38 samples out of 50 were positive by RT-LAMP and identified serotypes were A (n = 15), O (n = 15), and Asia-1 (n = 8). The efficiency of RT-LAMP in this study was highest for serotype Asia-1 (80.9%) followed by serotype A (73.4%) and O (62.35).

show abstract

Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 54%

See 1 more Smart Citation

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

Maryam¹,

Rasheed²,

Latif³

et al. 2017

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

“…The targeted viral genomes belonged to all classes of RNA virus of the Baltimore Classification except Class VI, with 8.5% of the studies reporting amplification of RNA fragments of Class III virus (dsRNA), 68.1% of Class IV virus ((+) ssRNA), 21.3% of Class V virus ((−) ssRNA), and 2.1% of the three classes mentioned above. An animal specimen source accounted for 38.3% of the reports (Moscoso et al, 2005;Maw et al, 2006;Moscoso et al, 2006;Perozo et al, 2006;Purvis et al, 2006;Inoue et al, 2007;Narayanan et al, 2010;Abdelwhab et al, 2011;Kraus et al, 2011;Keeler et al, 2012;Linhares et al, 2012;Madhanmohan et al, 2013;Awad et al, 2014;Biswal et al, 2016;Foss et al, 2016;Jozwiak et al, 2016;Madhanmohan et al, 2016;Manswr et al, 2018). Among them, twelve reports isolated RNA from viruses that can cause disease in poultry or wild birds, like Newcastle virus disease, avian influenza, or infectious bursal disease (Table 1).…”

Section: Resultsmentioning

confidence: 99%

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

View full text Add to dashboard Cite

A B S T R A C TBackgroundDetection and characterization of viral RNA pathogens from fieldwork are challenging due to the instability of the RNA molecule. FTA cards® have proved useful for sample storage and latter identification of pathogens with importance for agricultural, animal and human health: however, for optimal handling, processing, and biosafety measures are not well-established.ObjectiveThis systematic review aims to summarize the reported effectiveness of FTA cards® for storage and transport of viral RNA, as well as the conditions for their handling and use in downstream processes. Finally, the biosafety measures required to protect researchers and clinical lab workers are considered.MethodsWe performed a systematic review following the PRISMA statement. We searched MEDLINE (PubMed), Scopus and Web of Science using the keywords "FTA cards" AND "RNA". Articles were screened by title and abstract, and after examination of inclusion and exclusion criteria, relevant information was extracted. The quality of the studies was assessed, and the evidence was qualitatively summarized.ResultsA total of 175 records were retrieved, and 11 additional documents were found by checking references of the eligible articles. A total of 47 articles were included. Samples from animals accounted for 38.3% of the publications, which identified viruses that cause disease in poultry, wild birds, suids, or bovids. Three different methods for RNA extraction were reported. Other factors that vary across reports include the size of RNA amplicon, storage temperature, and duration of storage. Only fourteen articles tested the inactivation of the virus on the FTA card®, and in one case, the virus remained infective.ConclusionFTA cards® could be a suitable option for RNA virus storage and transport for fieldwork in areas where proper conditions for RNA preservation are difficult to achieve. Three different protocols have been used for RNA detection from this matrix. Biospecimens in the form of dried blood spots should be considered potentially infectious unless specifically treated to inactivate viral pathogens. technology consists of filter paper impregnated with a patented chemical mixture that contains chemical denaturants and a free radical scavenger. The chemical mixture lyses cells and organelles, prevents overgrowth of bacteria, and denatures proteins. The DNA is captured in the matrix and remains tightly bound while proteins and inhibitors are washed. This keeps DNA stable during long-term storage at room temperature (GE Healthcare, 2019).

show abstract

“…A number of RNA detection assays targeting the FMDV genome have been developed using reverse transcription loop‐mediated isothermal amplification (RT‐LAMP), with some detecting single serotypes and others multiple (Chen et al., ; Madhanmohan et al., ; Yamazaki et al., ; Ding et al., ; Kasanga et al., ). RT‐LAMP was faster, simpler, more cost‐effective and at least as sensitive and specific as RT‐PCR.…”

Section: Introductionmentioning

confidence: 99%

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Knight-Jones

Robinson

Charleston

et al. 2016

Transbound Emerg Dis

View full text Add to dashboard Cite

SummaryThis study assessed knowledge gaps in foot-and-mouth disease (FMD) research in the field of diagnostics. The study took the form of a literature review combined with research updates collected in 2014 from 33 institutes from around the world. Findings were used to identify priority areas for future FMD research.Molecular and genetic technologies, including sequencing, are developing at an increasing rate both in terms of capability and affordability. These advances potentiate progress in many other fields of research, from vaccine development to epidemiology. The development of RT-LAMP represents an important breakthrough allowing greater use and access to molecular diagnostics. It is now possible to determine virus serotype using PCR, although only for certain virus pools, continued progress is needed to cover the global spectrum of FMD viruses. Progress has also been made in the development of pen-side rapid diagnostics, some with the ability to determine serotype. However, further advances in pen-side serotype or strain determination would benefit both FMD-free countries and endemic countries with limited access to wellresourced laboratories.Novel sampling methods that show promise include air sampling and baited ropes, the latter may aid sampling in wildlife and swine. Studies of infrared thermography for the early detection of FMD have not been encouraging, although investigations are ongoing. Multiplex tests have been developed that are able to simultaneously screen for multiple pathogens with similar clinical signs. Crucial for assessing FMDV freedom, tests exist to detect animals that have been infected with FMDV regardless of vaccination status; however, limitations exist, particularly when testing previously vaccinated animals. Novel vaccines are being developed with complementary DIVA tests for this purpose. Research is also needed to improve the current imprecise approaches to FMD vaccine matching.The development of simple, affordable tests increases access to FMD diagnostics, greatly benefiting regions with limited laboratory capacity.

show abstract

Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid serotyping of foot-and-mouth disease virus

Cited by 28 publications

References 30 publications

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

One-step real-time loop-mediated isothermal amplification (RT-LAMP): evaluation and its application for the detection of foot-and-mouth-disease virus and its serotypes

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Contact Info

Product

Resources

About