Development and evaluation of an automated atlas-based image analysis method for microPET studies of the rat brain

Rubins, Daniel; Melega, William P.; Laćan, Goran; Way, Baldwin M.; Plenevaux, Alain; Luxen, André; Cherry, Simon R.

doi:10.1016/j.neuroimage.2003.07.011

Cited by 102 publications

(84 citation statements)

References 30 publications

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“…The plasma-free fraction was calculated as the ratio of activities (after correcting for decay) between the plasma filtrate and the spiked plasma, increased by correcting for the fraction of activity retained in the ultrafiltration device found for phosphate-buffered saline. 11 C]PBR28 rat brain template was generated by coregistration of six such scans to the cryo-image of Rubins et al 26 The template was then coregistered to each of the [ 11 C]PBR28 images in this study. The same transformation was applied to an eroded version of the corresponding brain atlas.…”

Section: C]pet28 Positron Emission Tomography Imagingmentioning

confidence: 99%

[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

Walker

Dinelle

Kornelsen

et al. 2015

J Cereb Blood Flow Metab

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Neuroinflammation in the aging rat brain was investigated using [11 C]PBR28 microPET (positron emission tomography) imaging. Normal rats were studied alongside LRRK2 p.G2019S transgenic rats; this mutation increases the risk of Parkinson's disease in humans. Seventy [ 11 C]PBR28 PET scans were acquired. Arterial blood sampling enabled tracer kinetic modeling and estimation of V T . In vitro autoradiography was also performed. PBR28 uptake increased with age, without differences between nontransgenic and transgenic rats. In 12 months of aging (4 to 16 months), standard uptake value (SUV) increased by 56% from 0.44 to 0.69 g/mL, whereas V T increased by 91% from 30 to 57 mL/cm 3 . Standard uptake value and V T were strongly correlated (r = 0.52, 95% confidence interval (CI) = 0.31 to 0.69, n = 37). The plasma free fraction, f p , was 0.21 ± 0.03 (mean ± standard deviation, n = 53). In vitro binding increased by 19% in 16 months of aging (4 to 20 months). The SUV was less variable across rats than V T ; coefficients of variation were 13% (n = 27) and 29% (n = 12). The intraclass correlation coefficient for SUV was 0.53, but was effectively zero for V T . These data show that [11 C]PBR28 brain uptake increases with age, implying increased microglial activation in the aged brain.

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Section: C]pet28 Positron Emission Tomography Imagingmentioning

confidence: 99%

[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

Walker

Dinelle

Kornelsen

et al. 2015

J Cereb Blood Flow Metab

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“…Further data processing and statistical analysis were performed by means of custom-made toolboxes implemented in statistical parametric mapping software SPM5 (Wellcome Department of Cognitive Neurology, London). All the individual [ 18 F]FDG lPET images were stereotactically normalized to a custommade [ 18 F]FDG template (average of 20 healthy rats), which was manually coregistered to a digital high-resolution cryosection-based atlas of rat brain (Toga et al 1995;Rubins et al 2003), using automated algorithms implemented in SPM5. All scans were analyzed after normalization to whole brain.…”

Section: Image Processing and Statistical Analysismentioning

confidence: 99%

Sequential [18F]FDG µPET whole-brain imaging of central vestibular compensation: a model of deafferentation-induced brain plasticity

et al. 2014

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Unilateral inner ear damage is followed by a rapid behavioural recovery due to central vestibular compensation. In this study, we utilized serial [(18)F]Fluoro-deoxyglucose ([(18)F]FDG)-µPET imaging in the rat to visualize changes in brain glucose metabolism during behavioural recovery after surgical and chemical unilateral labyrinthectomy, to determine the extent and time-course of the involvement of different brain regions in vestibular compensation and test previously described hypotheses of underlying mechanisms. Systematic patterns of relative changes of glucose metabolism (rCGM) were observed during vestibular compensation. A significant asymmetry of rCGM appeared in the vestibular nuclei, vestibulocerebellum, thalamus, multisensory vestibular cortex, hippocampus and amygdala in the acute phase of vestibular imbalance (4 h). This was followed by early vestibular compensation over 1-2 days where rCGM re-balanced between the vestibular nuclei, thalami and temporoparietal cortices and bilateral rCGM increase appeared in the hippocampus and amygdala. Subsequently over 2-7 days, rCGM increased in the ipsilesional spinal trigeminal nucleus and later (7-9 days) rCGM increased in the vestibulocerebellum bilaterally and the hypothalamus and persisted in the hippocampus. These systematic dynamic rCGM patterns during vestibular compensation, were confirmed in a second rat model of chemical unilateral labyrinthectomy by serial [(18)F]FDG-µPET. These findings show that deafferentation-induced plasticity after unilateral labyrinthectomy involves early mechanisms of re-balancing predominantly in the brainstem vestibular nuclei but also in thalamo-cortical and limbic areas, and indicate the contribution of spinocerebellar sensory inputs and vestibulocerebellar adaptation at the later stages of behavioural recovery.

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“…should be enough to register rat brains since their anatomy is quite similar provided the animals are within a certain weight range [19]. In our study, we created a template by registering and averaging all the images from group A to a single reference image.…”

Section: Statistical Parametric Mapping Rigid Body Transformationsmentioning

confidence: 99%

Detection of Visual Activation in the Rat Brain Using 2-deoxy-2-[18F]fluoro-d-glucose and Statistical Parametric Mapping (SPM)

et al. 2008

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Purpose: This study was designed to assess changes in brain glucose metabolism in rats after visual stimulation. Materials and methods: We sought to determine whether visual activation in the rat brain could be detected using a small-animal positron emission tomography (PET) scanner and 2-deoxy-2-[ 18 F]fluoro-D-glucose (FDG). Eleven rats were divided into two groups: (a) five animals exposed to ambient light and (b) six animals stimulated by stroboscopic light (10 Hz) with one eye covered. Rats were injected with FDG and, after 45 min of visual stimulation, were sacrificed and scanned for 90 min in a dedicated PET tomograph. Images were reconstructed by a threedimensional ordered subset expectation maximization algorithm (1.8 mm full width at half maximum). A region-of-interest (ROI) analysis was performed on 14 brain structures drawn on coronal sections. Statistical parametric mapping (SPM) adapted for small animals was also carried out. Additionally, the brains of three rats were sliced into 20-μm sections for autoradiography. Results: Analysis of ROI data revealed significant differences between groups in the right superior colliculus, right thalamus, and brainstem (p≤0.05). SPM detected the same areas as the ROI approach. Autoradiographs confirmed the existence of hyperactivation in the left superior colliculus and auditory cortex. Conclusions: To our knowledge, this is the first report that uses FDG-PET and SPM analysis to show changes in rat brain glucose metabolism after a visual stimulus.

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Development and evaluation of an automated atlas-based image analysis method for microPET studies of the rat brain

Cited by 102 publications

References 30 publications

[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

Sequential [18F]FDG µPET whole-brain imaging of central vestibular compensation: a model of deafferentation-induced brain plasticity

Detection of Visual Activation in the Rat Brain Using 2-deoxy-2-[18F]fluoro-d-glucose and Statistical Parametric Mapping (SPM)

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Development and evaluation of an automated atlas-based image analysis method for microPET studies of the rat brain

Cited by 102 publications

References 30 publications

[11C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

[11C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

Sequential [18F]FDG µPET whole-brain imaging of central vestibular compensation: a model of deafferentation-induced brain plasticity

Detection of Visual Activation in the Rat Brain Using 2-deoxy-2-[18F]fluoro-d-glucose and Statistical Parametric Mapping (SPM)

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[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat

[¹¹C]PBR28 PET Imaging is Sensitive to Neuroinflammation in the Aged Rat