Development and evaluation of live influenza (LIV) cold-adapted reassortant vaccines in cell culture

Kiseleva, Irina; Su, Qin; Toner, Timothy J.; Szymkowiak, C.; Kwan, W.-S; Kraiouchkine, Nikolai; Rudenko, Larisa; Shaw, Alan; Youil, Rima

doi:10.1016/j.ics.2004.02.158

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Co-infection step plays a key role in the classical genetic reassortment. Multiple studies have attempted to evaluate reassortment efficiencies of wild-type and cold-adapted viruses using different variants of crossing procedures, such as simultaneous [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] or successive [27][28][29] inoculation of two parental viruses. Co-infection procedures also differed by infectivity ratio of the viruses, temperature of incubation and culturing substrate (eggs or cell culture).…”

Section: Co-infection Proceduresmentioning

confidence: 99%

“…Co-infection procedures also differed by infectivity ratio of the viruses, temperature of incubation and culturing substrate (eggs or cell culture). Most of the recent studies describe reassortment of live parental viruses [11][12][13][14][15]20,[22][23][24][25][26]. However, the reassortment efficiency can be substantially improved if one of the parental viruses is inactivated prior to co-infection.…”

Section: Co-infection Proceduresmentioning

confidence: 99%

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New Methodological Approaches in The Development of Russian Live Attenuated Vaccine for Pandemic Influenza

Kiseleva¹,

Larionova²,

Федорова³

et al. 2015

Transl Biomed

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Avian influenza viruses remain a major pandemic threat. In response to this threat, a number of pandemic vaccines have been developed. The objective of this paper is to review and summarize data from preclinical and clinical evaluation of Russian live attenuated influenza vaccines (LAIVs) against pandemic influenza based on cold-adapted A/Leningrad/134/17/57 (H2N2) master donor virus (MDV). The described LAIVs consist of reassortant viruses of 6:2 and 7:1 genomic composition (6 MDV genes: 2 WT genes and 7 MDV genes: 1 WT gene, respectively). Despite the differences in their genomic composition (6:2 or 7:1), LAIV candidates of H5, H7 and H2 subtypes acquired temperature sensitivity, cold-adaptation, and attenuation for different animal models. In addition, they were safe and immunogenic for healthy adult volunteers. The collected data indicate that 7:1 reassortants carrying HA genes of potentially pandemic viruses and the remaining genes from the MDV might be preferable pandemic LAIV candidates.

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