Development and Evaluation of Paclitaxel-Loaded Aerosol Nanocomposite Microparticles and Their Efficacy Against Air-Grown Lung Cancer Tumor Spheroids

Guzmán, Elisa A. Torrico; Sun, Qihua; Meenach, Samantha A.

doi:10.1021/acsbiomaterials.9b00947

Cited by 21 publications

(8 citation statements)

References 61 publications

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“…From this study, it was observed that the fine particle fraction (FPF) was 50.86 ± 2.80%, which is suitable for effective delivery to the lungs [9]. These particles would likely settle in the lower region of lung airways due to sedimentation (0.5-5 µm), whereas Brownian motion (0.5 µm) would cause stage 7 particles to settle in the alveoli [25]. However, the concentration of drug retained in the pre-separator is substantial with almost 22%, indicating scope for reducing this loss further by decreasing the size of the PLMs.…”

Section: Discussionmentioning

confidence: 94%

“…The drugs can be administered directly to the lung through oral inhalation, limiting systemic exposure, making it a promising noninvasive delivery route [7]. There are reports of DPIs of PTX prepared by spray-drying methods for lung cancer [22,25]. These systems have been found to be effective in the delivery of the drug through this system.…”

Section: Introductionmentioning

confidence: 99%

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Development of Paclitaxel Proliposomal Dry Powder Inhaler (PTX-PLM-DPI) by Freeze-Drying Method for Lung Cancer

Borah,

Saikia,

Bharali

et al. 2024

DDC

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Despite various efforts, a successful selective delivery system for chemotherapeutic agents for lung cancer is still lacking. Dry powder inhaler (DPI) systems based on proliposomes (PLMs) could be a potential system for the efficient delivery of paclitaxel to lungs. PLM-based DPI prepared with a freeze-drying method can therefore be an alternative. Paclitaxel-loaded PLM-based DPI (PTX-PLM-DPI) powders were prepared using the method of thin film deposition on a carrier followed by freeze drying. These were prepared using soya phosphatidylcholine (SPC) and cholesterol as the lipids and mannitol as the carrier. The reconstituted liposomes were evaluated in terms of size, morphology, drug entrapment, release and cytotoxicity. The DPI powders were evaluated for their flow property, surface topography, dose uniformity and in vitro lung deposition. Stable and free-flowing PTX-PLM-DPI powder was obtained that could be reconstituted into homogenous liposomal vesicles < 200 nm as confirmed by TEM and SEM studies. The liposomes showed drug entrapment of 92.64 ± 1.4% and diffusion-controlled release of up to 28% in 24 h. These liposomes showed better dose-dependent cytotoxicity in A549 cells in comparison to paclitaxel suspension with IC50 values of 46 ± 0.87 ng/mL and 154.9 ± 3.64 ng/mL, respectively. In vitro lung deposition studies of the PTX-PLM-DPI showed sufficient deposition with the fine particle fraction (FPF) of 50.86 ± 2.8% of particles with an aerodynamic diameter less than 5 µ. Hence, it canbe concluded that PLM-based DPI prepared by freeze drying can be a promising, stable, safe and free-flowing system for the enhanced lung delivery of paclitaxel.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of Paclitaxel Proliposomal Dry Powder Inhaler (PTX-PLM-DPI) by Freeze-Drying Method for Lung Cancer

Borah,

Saikia,

Bharali

et al. 2024

DDC

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“…A Millipore water system (Direct Q @ UV) provided high purity of water that used for all experiments. A549 human lung adenocarcinoma epithelial cells were obtained and cultured as previously described …”

Section: Methodsmentioning

confidence: 99%

“…A549 human lung adenocarcinoma epithelial cells were obtained and cultured as previously described. 54 Preparation of [ML P ] and [ML P -DOX].…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Radiofrequency and Near-Infrared Responsive Core–Shell Nanostructures Using Layersome Templates for Cancer Treatment

Pan

Jakaria

Meenach

et al. 2019

ACS Appl. Bio Mater.

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We report a multifunctional nanotherapeutic platform based on liposomes loaded with drug and iron oxide nanoparticles (IONs) coated with a gold nanoshell synthesized using a polyelectrolyte (layersome) soft templating technique. IONs and gold nanoshells were used to provide combined hyperthermia and triggered drug release via radio frequency (RF) or near-infrared (NIR) stimulation. IONs and the anticancer drug doxorubicin (DOX) were coencapsulated inside liposomes composed of zwitterionic phosphatidylcholine, anionic phosphatidylglycerol, and cholesterol lipids. Coating the magneto-liposomes with positively charged poly-l-lysine enriched the interface with gold anions to form a dense gold nanoshell and protected the structure against deformation and DOX cargo release during shell formation. After modification with thiol-terminated polyethylene glycol, intracellular delivery and release of DOX from the nanostructures was examined in A549 human lung cancer cells. The nanostructures retained their DOX cargo and remained in the cytosol after cellular uptake. Only when triggered by RF or NIR stimuli did the nanostructures release DOX, which then entered the cell nucleus. Compared to the single photothermal therapy or radio frequency treatment, the carriers with combined DOX and RF or NIR stimulation displayed higher therapeutic effect on A549 cells.

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“…Nanocarriers have been developed for PTX, as the hydrophobicity of PTX reduces its bioavailability. Recently, paclitaxel-loaded aerosol nanoparticles were synthesized and delivered to the lungs through a dry powder inhaler for treating NCSLC [79]. The size and morphology of the nanoparticles made them accessible to the distal region of the lungs.…”

Section: Paclitaxel-loaded Aerosol Nanoparticles For Drug Delivery In Lung Cancermentioning

confidence: 99%

Recent Advances and Implication of Bioengineered Nanomaterials in Cancer Theranostics

et al. 2021

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Cancer is one of the most common causes of death and affects millions of lives every year. In addition to non-infectious carcinogens, infectious agents contribute significantly to increased incidence of several cancers. Several therapeutic techniques have been used for the treatment of such cancers. Recently, nanotechnology has emerged to advance the diagnosis, imaging, and therapeutics of various cancer types. Nanomaterials have multiple advantages over other materials due to their small size and high surface area, which allow retention and controlled drug release to improve the anti-cancer property. Most cancer therapies have been known to damage healthy cells due to poor specificity, which can be avoided by using nanosized particles. Nanomaterials can be combined with various types of biomaterials to make it less toxic and improve its biocompatibility. Based on these properties, several nanomaterials have been developed which possess excellent anti-cancer efficacy potential and improved diagnosis. This review presents the latest update on novel nanomaterials used to improve the diagnostic and therapeutic of pathogen-associated and non-pathogenic cancers. We further highlighted mechanistic insights into their mode of action, improved features, and limitations.

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Development and Evaluation of Paclitaxel-Loaded Aerosol Nanocomposite Microparticles and Their Efficacy Against Air-Grown Lung Cancer Tumor Spheroids

Cited by 21 publications

References 61 publications

Development of Paclitaxel Proliposomal Dry Powder Inhaler (PTX-PLM-DPI) by Freeze-Drying Method for Lung Cancer

Development of Paclitaxel Proliposomal Dry Powder Inhaler (PTX-PLM-DPI) by Freeze-Drying Method for Lung Cancer

Radiofrequency and Near-Infrared Responsive Core–Shell Nanostructures Using Layersome Templates for Cancer Treatment

Recent Advances and Implication of Bioengineered Nanomaterials in Cancer Theranostics

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