Development and in Vitro Evaluation of Valsartan-Loaded Resealed Erythrocytes

Elhassan, Gamal O.; MOHAMED, JAMAL MOIDEEN MUTHU

doi:10.22159/ijap.2022.v14ti.57

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…The erythemal scores were recorded in control and test group for all the animals. The mean erythemal scores were found to be 0.00, which means that there was no edema or erythema on the skin of the shaved rats in the optimized formulation [32].…”

Section: Skin Irritationmentioning

confidence: 91%

Application of 32 Factorial Design of Loratadine-Loaded Nanosponge in Topical Gel system: In Vitro, Ex Vivo, and In Vivo Assessments

Sivadasan,

Venkatesan,

Mohamed

et al. 2024

Preprint

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Loratadine (LoR) is a highly lipophilic and practically insoluble in water, hence having a low oral bioavailability. As it is formulated as topical gel, it competitively binds with the receptors, thus reducing the side-effects. The objective of this study was to prepare LoR loaded nanosponge (LoR-NS) in gel for topical delivery. Nine different formulations of emulsion were prepared by solvent evaporation method with polyvinyl alcohol (PVA), ethyl cellulose (EC), and dichloromethane (DCM). Based on 32 Full Factorial Design (FFD), optimization was carried out by varying the concentration of LOR:EC ratio and stirring rate. The preparations were subjected for the evaluation of particle size (PS), in vitro release, zeta potential (ZP) and entrapment efficiency (EE). The results revealed that the NS dispersion was nanosized with sustained release profiles and significant PS. The optimized formulation was formulated and incorporated into carbopol 934P hydrogel. The formulation was then examined to surface morphological characterizations using scanning electron microscopy (SEM) which depicted spherical NS. Stability studies, undertaken for 2 months at 40 ± 2 ℃ and 75 ± 5% RH, concluded to the stability of the formulation. The formulation did not cause skin irritation. Therefore, the prepared NS hydrogel proved to be a promising applicant for LoR as a novel drug delivery system (NDDS) for safe, sustained and controlled topical application.

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Section: Skin Irritationmentioning

confidence: 91%

Application of 32 Factorial Design of Loratadine-Loaded Nanosponge in Topical Gel system: In Vitro, Ex Vivo, and In Vivo Assessments

Sivadasan,

Venkatesan,

Mohamed

et al. 2024

Preprint

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“…The slope of the linear part of the figure was used to compute the transdermal drug flow. The permeation kinetics was established as zero and first order, Korsmeyer-Peppas and Higuchi and as described by the previous method 23 .…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of a curcumin nanoemulsion gel for the effective treatment of mycoses

Al Fatease,

Alqahtani,

Khan

et al. 2023

Sci Rep

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Fungal infections of skin including mycoses are one of the most common infections in skin or skins. Mycosis is caused by dermatophytes, non-dermatophyte moulds and yeasts. Various studies show different drugs to treat mycoses, yet there is need to treat it with applied drugs delivery. This study was designed to prepare a bio curcumin (CMN) nanoemulsion (CMN-NEs) for transdermal administration to treat mycoses. The self-nanoemulsification approach was used to prepare a nanoemulsion (NE), utilizing an oil phase consisting of Cremophor EL 100 (Cre EL), glyceryl monooleate (GMO), and polyethylene glycol 5000 (PEG 5000). Particle size (PS), polydispersity index (PDI), zeta potential (ZP), Fourier transform infrared (FTIR) spectrophotometric analysis, and morphological analyses were performed to evaluate the nanoemulsion (NE). The in vitro permeation of CMN was investigated using a modified vertical diffusion cell with an activated dialysis membrane bag. Among all the formulations, a stable, spontaneously produced nanoemulsion was determined with 250 mg of CMN loaded with 10 g of the oil phase. The average droplet size, ZP, and PDI of CMN-NEs were 90.0 ± 2.1 nm, − 7.4 ± 0.4, and 0.171 ± 0.03 mV, respectively. The release kinetics of CMN differed from zero order with a Higuchi release profile as a result of nanoemulsification, which also significantly increased the flux of CMN permeating from the hydrophilic matrix gel. Overall, the prepared nanoemulsion system not only increased the permeability of CMN but also protected it against chemical deterioration. Both CMN-ME (24.0 ± 0.31 mm) and CMN-NE gel (29.6 ± 0.25 mm) had zones of inhibition against Candida albicans that were significantly larger than those of marketed Itrostred gel (21.5 ± 0.34 mm). The prepared CMN-NE improved the bioavailability, better skin penetration, and the CMN-NE gel enhanced the release of CMN from the gel matrix on mycotic patients.

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“…MEM-NC3 was spherical with a crystalline surface and a PS in the nanometric range was confirmed (Figure 2b). The formation of an encircling crystal network by a thin polymer layer during hydration enables the system to transform from globular to spherical and achieve thermodynamic stability by lowering total free energy [32]. The stability of the spherical structure was demonstrated even when several mechanical stressors, such as sonication and ultracentrifugation, were used.…”

Section: Surface Morphology Of Mem-nc3 By Sem Depicts Smooth Spherica...mentioning

confidence: 99%

Preparation of Memantine-Loaded Chitosan Nanocrystals: In Vitro and Ex Vivo Toxicity Analysis

Saleh

Mohamed²,

Ruby³

et al. 2022

Crystals

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Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with unmet medical need, and is the leading cause of age-related dementia affecting millions of people worldwide. This work aims at developing small, high-drug loading capacity (DL) and -entrapment efficiency (EE) memantine hydrochloride (MEM)/chitosan nanocrystals (CS-NCs) to treat moderate to severe dementia associated with AD. MEM-loaded chitosan nanocrystals (MEM/CS-NCs, further abbreviated as MEM-NCs) were prepared by the ionic gelation method. Different formulations were prepared by varying the concentrations of CS and sodium tripolyphosphate (STPP). The prepared MEM-NCs formulations (n = 8) were evaluated for their particle size (PS), polydispersibility index (PDI), zeta potential (ZP), DL, EE and characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Furthermore, in vitro (i.e., release behavior, cytotoxicity) and ex vivo studies (i.e., histopathology) studies were carried out. The results show that the DL was over 92% and the EE was higher than 73%, while the particles were relatively small with nanometric PS (152.63 ± 12.95 to 310.23 ± 10.49 nm), uniform with acceptable PDI (0.336 ± 0.05 to 0.534 ± 0.02), and stable with positive ZP (23.8 ± 0.4 mV to 54.0 ± 0.5 mV). The optimal formulation (MEM-NC3) was selected mainly based on the PS (152.63 ± 12.95 nm), DL (98.44 ± 3.31%), and EE (78.7 ± 3.11%). Interestingly, it does not elicit any cytotoxic and tissue damage when examining at goat nasal mucosa. The selected formulation was subjected to surface morphological studies such as transmission electron microscopy (TEM), which revealed that the NCs were spherical in shape and small (100 nm). Interestingly, the selected formulation was able to sustain the drug release for up to 24 h with an initial burst release (86.51 %). We conclude that the prepared MEM-NCs represent a promising drug formulation for further in vivo studies (in animal models and in a clinical setting) to prevent and treat AD.

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Development and in Vitro Evaluation of Valsartan-Loaded Resealed Erythrocytes

Cited by 4 publications

References 12 publications

Application of 32 Factorial Design of Loratadine-Loaded Nanosponge in Topical Gel system: In Vitro, Ex Vivo, and In Vivo Assessments

Application of 32 Factorial Design of Loratadine-Loaded Nanosponge in Topical Gel system: In Vitro, Ex Vivo, and In Vivo Assessments

Preparation and characterization of a curcumin nanoemulsion gel for the effective treatment of mycoses

Preparation of Memantine-Loaded Chitosan Nanocrystals: In Vitro and Ex Vivo Toxicity Analysis

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