Development and initial characterization of a chemically stabilized elastin-glycosaminoglycan-collagen composite shape-memory hydrogel for nucleus pulposus regeneration

Mercuri, Jeremy; Addington, Caroline P.; Pascal, Richard; Gill, Sanjitpal S.; Simionescu, Dan T.

doi:10.1002/jbm.a.35104

Cited by 18 publications

(17 citation statements)

References 50 publications

(58 reference statements)

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“…To fabricate elastin‐based porous scaffolds (hydrogels) with desirable properties for tissue engineering, various methods including gas foaming and freeze‐drying have been employed ( Figure ) …”

Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

“…Freeze‐drying is another technique for the fabrication of highly porous elastin‐based composite scaffolds . Freezing a dispersion or solution results in the formation of ice crystals, which are subsequently removed by freeze‐drying to leave pores inside the material.…”

Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

“…Pore size is inversely related to the freezing rate To illustrate, a tropoelastin‐collagen solution frozen at ‐ 80 °C and freeze‐dried produces porous disc‐shaped or tubular scaffolds . Likewise, an elastin‐glycosaminoglycan‐collagen composite hydrogel has been manufactured by gelation at 37 °C, followed by lyophilization and cross‐linking with 1‐ethyl‐3‐(3 dimethylaminopropyl)carbodiimide/ N ‐hydroxysuccinimide (EDC/NHS) . Similarly, silk‐elastin hydrogels have been made by freeze‐drying genipin‐cross‐linked samples, and further stabilizing them via methanol immersion .…”

Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

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Fabricated Elastin

Yeo

Aghaei-Ghareh-Bolagh

Brackenreg

et al. 2015

Adv Healthcare Materials

103

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The mechanical stability, elasticity, inherent bioactivity, and self-assembly properties of elastin make it a highly attractive candidate for the fabrication of versatile biomaterials. The ability to engineer specific peptide sequences derived from elastin allows for precise control of these physicochemical and organizational characteristics, and further broadens the diversity of elastin-based applications. Elastin and elastin-like peptides can also be modified or blended with other natural or synthetic moieties, including peptides, proteins, polysaccharides and polymers, to augment existing capabilities or confer additional architectural and biofunctional features to compositionally pure materials. Elastin and elastin-based composites have been subjected to diverse fabrication processes, including heating, electrospinning, wet spinning, solvent casting, freeze-drying, and cross-linking, for the manufacture of particles, fibers, gels, tubes, sheets and films. The resulting materials can be tailored to possess specific strength, elasticity, morphology, topography, porosity, wettability, surface charge and bioactivity. This extraordinary tunability of elastin-based constructs enables their use in a range of biomedical and tissue engineering applications such as targeted drug delivery, cell encapsulation, vascular repair, nerve regeneration, wound healing, and dermal, cartilage, bone and dental replacement.

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Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

Section: Composite Elastin‐based Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Fabricated Elastin

Yeo

Aghaei-Ghareh-Bolagh

Brackenreg

et al. 2015

Adv Healthcare Materials

103

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“…Hence, these are frequently included in possible tissue engineering treatment approaches (Priyadarshani et al, 2015), e.g. agarose (Awad et al, 2004;Bougault et al, 2012;Iwata et al, 2013;Luo et al, 2015;Mauck et al, 2006), collagen (Borde et al, 2015;Calderon et al, 2010;Mercuri et al, 2014;Omlor et al, 2012;Tsaryk et al, 2015), alginate (Bron et al, 2011;Duggal et al, 2009;Xu et al, 2008;Zeng et al, 2015a), chitosan (Naqvi and Buckley, 2015;Ngoenkam et al, 2010), and combinations of alginate and chitosan have been investigated (Shao , 2007). Although natural hydrogels generally offer good cytocompatibility, they often possess inferior mechanical properties (Schutgens et al, 2015).…”

Section: Reinforced Hydrogels For Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Frauchiger¹,

Tekari²,

Wöltje³

et al. 2017

eCM

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The degeneration of the intervertebral disc (IVD) within the spinal column represents a major pain source for many patients. Biological restoration or repair of the IVD using "compressive-force-resistant" and at the same time "cytocompatible" materials would be desirable over current purely mechanical solutions, such as spinal fusion or IVD implants. This review provides an overview of recent research on the repair of the inner (nucleus pulposus = NP) and the outer (annulus fibrous = AF) parts of the IVD tissue. Many studies have addressed NP repair using hydrogel-like materials. However, only a few studies have so far focused on AF repair. As the AF possesses an extremely low self-healing capacity and special attention to shear-force resistance is essential, special repair designs are required. In our review, we stated the challenges in IVD repair and highlighted the use of composite materials such as silk biomaterials and fibrin cross-linked reinforced hydrogels. We elaborated on the origin of silk and its many in tissue engineering. Furthermore, techniques such as electrospinning and 3D printing technologies allow the fabrication of versatile and functionalised 3D scaffolds. We summarised the research that has been conducted in the field of regenerative medicine over the recent years, with a special focus on the potential application and the potential of combining silk and reinforced -and thus mechanically tailored -hydrogels for IVD repair.

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“…Native caprine NP and AF cells successfully migrated into 3.0 % collagen type I scaffolds, which was dependent on collagen digestion by the migrating cells (Bron et al, 2012). Human adiposederived stromal cells seeded on elastin-glycosaminoglycancollagen I composite hydrogels attained an NP-like morphology (Mercuri et al, 2014). Gelatin, mostly derived from collagen I, is often used in composite hydrogels to improve biomechanical properties.…”

Section: Collagen and Gelatinmentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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Development and initial characterization of a chemically stabilized elastin-glycosaminoglycan-collagen composite shape-memory hydrogel for nucleus pulposus regeneration

Cited by 18 publications

References 50 publications

Fabricated Elastin

Fabricated Elastin

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

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