Development and maturation of the immune system in zebrafish, Danio rerio: a gene expression profiling, in situ hybridization and immunological study

Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation

“…In past several years, extensive studies have been performed on the distribution of IgM-producing cells in lymphoid tissues in zebrafish (Lam et al, 2004), cod (Stenvika et al, 2001), spotted wolffish (Anarhichas minor) and channel catfish (Patrie-Hanson and Ainsworth 2001). However, there has been little information available on the distribution of IgD-and IgT/IgZproducing cells in lymphoid tissues in any species of fish, and their gene expression in response to pathogenic infection has seldom been reported.…”

Regard à travers le danio pour mieux comprendre les interactions hôte/pathogène

“…Alors que la réponse adaptative n'est fonctionnelle qu'après 4 à 6 semaines de développement, la branche innée est la seule composante fonctionnelle au stade embryonnaire précoce, avec la présence dans la circulation sanguine de macrophages actifs dès 25 hpf (heures post-fécondation) et de granulocytes dès 30 hpf (Figure 1). Il apparaît dès lors possible de découpler les deux systèmes, et d'en étudier la contribution propre au cours d'un processus infectieux [10]. Le zebrafish offre aujourd'hui de nombreux outils pour des études de biologie cellulaire, moléculaire et génétique, dont les informations sont regroupées dans des bases de données publiques 1 .…”

Molecular multi‐effect screening of environmental pollutants using the MolDarT

“…Sequences for b-actin forward primer and probe are from Trant et al (2001); primer sequences for rag1 are from Lam et al (2004).…”

“…Developmental rag1 expression has been described using real-time PCR (Lam et al, 2004). These authors describe an induction of developmentally expressed rag1 between 24 and 72 hpf, when transcripts are first detected, and a steady increase in rag1 transcript abundance until Fig.…”

“…Rag1 is crucial for acquired immunity, since it is involved in V(D)J recombination of antibody and T-cell receptor genes (Abbas et al, 1994). The zebrafish rag1 gene has been cloned (Willett et al, 1997a) and its expression in the developing zebrafish has been characterized (Willett et al, 1997b;Lam et al, 2004), with the first transcripts detected at the third day past fertilization (dpf). Lack of functional rag1 genes leads to complete absence of mature B-and Tcells, both in mice (Mombaerts et al, 1992) and zebrafish (Wienholds et al, 2002).…”