2008
DOI: 10.1002/tox.20305
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Molecular multi‐effect screening of environmental pollutants using the MolDarT

Abstract: Molecular effect detection is a useful approach for ecotoxicological screening of chemicals. We show here the application of the molecular DarT (MolDarT), where the expression of selected target genes is detected in short-term (120 h) exposed developing zebrafish (Danio rerio), thus allowing subacute multi-effect compound screening. The genes metallothionein 2 (mt2), cytochrome P450 1A1 (cyp1a1), and recombination activation gene 1 (rag1) are used as endpoints that describe detoxification/metal toxicity (mt2),… Show more

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Cited by 27 publications
(7 citation statements)
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“…Nonylphenol, a xenoestrogen first detected in sewage of water treatment plants in 1984 [17] has been extensively studied as one of the most potent environmental chemicals (EDCs) [13], [45], [46]. There are accumulated data on negative effects of human exposure [47] and in animal models endocrine and reproductive effects of nonylphenol have been shown such as inhibited testicular growth [48], [49], lower semen volume [50] and lower fertility [51].…”
Section: Discussionmentioning
confidence: 99%
“…Nonylphenol, a xenoestrogen first detected in sewage of water treatment plants in 1984 [17] has been extensively studied as one of the most potent environmental chemicals (EDCs) [13], [45], [46]. There are accumulated data on negative effects of human exposure [47] and in animal models endocrine and reproductive effects of nonylphenol have been shown such as inhibited testicular growth [48], [49], lower semen volume [50] and lower fertility [51].…”
Section: Discussionmentioning
confidence: 99%
“…The assessment of multiple sublethal endpoints in the FET test is considered a potentially fruitful approach to discover suitable chronic toxicity predictors. Suggested endpoints include gene expression changes (Liedtke et al, 2008;Schiller et al, 2013;Weil et al, 2009), assessment of target organ toxicity and morphological abnormalities (Embry et al, 2010;Lammer et al, 2009;Nagel, 2002;Sipes et al, 2011;Volz et al, 2011), as well as behavioural alterations (Sloman and McNeil, 2012). The assessment of the capacity of various sublethal endpoints assessed in the FET test setup to predict chronic toxicity in FELS tests forms already an area of active research (Villeneuve et al, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…Nagel (2002) outlined these toxicity indicators as measured using an inverse light microscope based on “yes” or “no” responses to the presence of the four factors [ 60 ]. On the other hand, Hermsen et al [ 61 ] introduced a new model of embryotoxicity (ZET) using the general morphology score (GMS). A separate scoring list was established for teratogenic impact, which helped track slow progress, developmental delay, and teratogenicity.…”
Section: Zebrafish As a Suitable Alternative Animal Model For Toximentioning
confidence: 99%
“…However, the ZET, along with GMS, operates as an essential and useful test method for screening the chemicals’ embryotoxic properties in the compound groups studied [ 27 ]. Furthermore, the MolDarT was created with zebrafish eggs/larvae to develop a molecular mechanism test method [ 61 ]. It is based in theory on the DarT ( Danio rerio teratogenicity test), established by Nagel et al [ 60 ], which reveals and tracks the developmental impact and deformities of freshly fertilized zebrafish eggs within 48 h [ 62 ].…”
Section: Zebrafish As a Suitable Alternative Animal Model For Toximentioning
confidence: 99%