Zebrafish as a Successful Animal Model for Screening Toxicity of Medicinal Plants

Abstract

The zebrafish (Danio rerio) is used as an embryonic and larval model to perform in vitro experiments and developmental toxicity studies. Zebrafish may be used to determine the toxicity of samples in early screening assays, often in a high-throughput manner. The zebrafish embryotoxicity model is at the leading edge of toxicology research due to the short time required for analyses, transparency of embryos, short life cycle, high fertility, and genetic data similarity. Zebrafish toxicity studies range from assessing the toxicity of bioactive compounds or crude extracts from plants to determining the optimal process. Most of the studied extracts were polar, such as ethanol, methanol, and aqueous solutions, which were used to detect the toxicity and bioactivity. This review examines the latest research using zebrafish as a study model and highlights its power as a tool for detecting toxicity of medicinal plants and its effectiveness at enhancing the understanding of new drug generation. The goal of this review was to develop a link to ethnopharmacological zebrafish studies that can be used by other researchers to conduct future research.

Self‐assembly of methoxy poly(ethylene glycol)‐cholesterol micelles for controlled quercetin delivery with toxicity test inDanio reriomodel

“…For the former, zebrafish organs, tissues and especially the genome that can resemble some of those in human, which are interested to exert toxicological tests prior to clinical trials on human 56 . Concerning the latter, utilizing this cold‐blooded animal as testing model can exclude the time and effort‐consuming process for registration and license following animal welfare act in research, creating better chances for high through put screening, testing of toxicological experiments 57 . The immersion treatment of zebrafish in QCT, blank micelles and QCT‐M produced clear histological changes in gills, while intestine and liver only experienced mild alterations of tissues at high concentrations.…”

Comparative Toxicity Assessment of Kratom Decoction, Mitragynine and Speciociliatine Versus Morphine on Zebrafish (Danio rerio) Embryos

“…Morphological malformations such as spinal curvature, yolk oedema, and pericardial oedema are important sublethal parameters observed in zebrafish embryos when exposed to toxic chemicals (Chahardehi et al, 2020). An example, drugs such as alcohol and nicotine that are known to affect human fetal development have been reported to induce morphological defects in the zebrafish embryos (Lantz-McPeak et al, 2015).…”

Jackfruit Seed Extract Exposure on Zebrafish Embryos as Initial Screening Model for Covid-19 Treatment

“…This stage determines the cell movement during gastrulation [21]. Exposure to toxic substances at this stage can lead to malformation in embryos and larvae [22]. Observation on early embryo development (24 hpf) showed no effect of Jackfruit Seed Extract (JSE) and showed no coagulation of fertilized eggs.…”