Development and Optimisation of HILIC-LC-MS Method for Determination of Carbohydrates in Fermentation Samples

Abstract

Saccharides are the most common carbon source for Streptococcus thermophilus, which is a widely used bacterium in the production of fermented dairy products. The performance of the strain is influenced by the consumption of different saccharides during fermentation. Therefore, a precise measurement of the concentrations of saccharides in the fermentation media is essential. An 18-min long method with limits of quantitation in the range of 0.159–0.704 mg/L and with 13C labelled internal standards employing hydrophilic interaction chromatography coupled to mass spectrometric detection-(HILIC-LC-MS) allowed for simultaneous quantification of five saccharides: fructose, glucose, galactose, sucrose, and lactose in the fermentation samples. The method included a four-step sample preparation protocol, which could be easily applied to high-throughput analysis. The developed method was validated and applied to the fermentation samples produced by Streptococcus thermophilus.

Structural Elucidation and Activities of Cordyceps militaris-Derived Polysaccharides: A Review

“…Permethylation is an efficient method to improve the stability of glycans. Hydrophilic interaction chromatography coupled with mass spectrometric detection (HILIC-MS) is also used to sequence carbohydrates ( 40 , 41 ). Moreover, HPLC-MS was recently used to detect the metabolites of polysaccharides as well as their interaction with proteins ( 42 ).…”

Research of saccharides and related biocomplexes: A review with recent techniques and applications

“…HILIC on-line coupled with TOF-MS aids measurements of neutral saccharides and the determination of their quantitative profiles simultaneously with selective mass fragments [90,[177][178][179]. Separation is needed to realize a selective roadmap for the identification of the monosaccharides fructose, glucose, and galactose, the disaccharides sucrose and melibiose, and the oligosaccharides raffinose, mannotriose, stachyose, and verbascose [28,91,180].…”

Quantification methods for carbohydrate compounds in biologicals: a review

“…Масс-спектрометрический способ детектирования в тандеме с жидкостной или газовой хроматографией [27] обладает большей чувствительностью, однако не является оптимальным с экономической точки зрения и не встречается в оценке качества БЛП для определения вспомогательных веществ углеводной природы, зарегистрированных в Российской Федерации.…”