Development and optimization of a system for comprehensive two-dimensional liquid chromatography with UV and mass spectrometric detection for the separation of complex samples by multi-step gradient elution

Evaluation of hydrophilic interaction chromatography (HILIC) versus C18 reversed-phase chromatography for targeted quantification of peptides by mass spectrometry

“…Resolution between peptides and peak capacity of the chromatographic system were also calculated in order to evaluate the quality of the separation. The peak capacity in gradient mode is calculated as follows [28]:…”

Comprehensive liquid chromatography: Fundamental aspects and practical considerations—A review

“…Despite the longer regeneration times, these columns are considered beneficial in terms of analysis time for separations requiring less than 30,000 theoretical plates [71,88] as is the case for the second dimension in comprehensive LC [51,79,87,[89][90][91][92][93][94][95][96][97][98][99][100][101]. Obviously, a decrease of the particle size results in enhanced resolution, but places strict requirements on instrumentation as well as on stationary phase stability.…”

LC coupled to ESI, MALDI and ICP MS – A multiple hyphenation for metalloproteomic studies

“…The hyphenated systems should also exhibit minimal sample loss, minimal contamination by sample handling, sufficient repeatability and high throughput [6]. A range of multidimensional separations combining diverse chromatographic and electrophoretic techniques has been described and is routinely used [7]. Without doubt, liquid chromatography (LC) is the most frequently used separation technique for biological applications [7].…”

“…A range of multidimensional separations combining diverse chromatographic and electrophoretic techniques has been described and is routinely used [7]. Without doubt, liquid chromatography (LC) is the most frequently used separation technique for biological applications [7]. Combinations of chromatographic techniques, such as strong cation exchange chromatography-reversed phase liquid chromatography (RPLC), size-exclusion chromatography, RPLC-RPLC and hydrophilic interaction chromatography-RPLC in on-line or off-line arrangements have been used up to now [6].…”