Development and use of detection methods specific for Cucumber vein yellowing virus (CVYV)

Martínez‐García, Belén; Marco, Cristina F.; Goytia, Elisa; López‐Abella, D.; Serra, María Teresa; Aranda, Miguel A.; López‐Moya, Juan José

doi:10.1007/s10658-004-2491-7

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…We tested 135 C. melo and 12 wild relative accessions of the germplasm collection of Estación Experimental "La Mayora"- CSIC (Málaga, Spain) for their susceptibility to MNSV strains Mα5 (MNSV-Mα5, avirulent on melons of nsv/nsv genotype) [33] and 264 (MNSV-264, virulent on melons of the nsv/nsv genotype) [26] and to Cucumber vein yellowing virus (CVYV) [34]. Accessions were from different geographical origins: 3 from Africa, 7 from America, 17 from Central Asia, 90 from Europe (4 from Central Europe, 74 from Spain and 13 from other southern European regions), 3 from the Far East and India, 12 from Middle East and the remaining 14 from unknown origins (see Additional file 1).…”

Section: Resultsmentioning

confidence: 99%

“…Presence or absence of virus symptoms was recorded for each test plant at 7, 15 and 25 days after inoculation. Then, in two symptomatic plants per accession and in all asymptomatic plants or with no clear symptoms, presence of CVYV or MNSV was analysed by molecular hybridisation in tissue prints of cross sections of petioles from young leaves [45] using probes decribed in [33,34]. Ten plants per accession and virus combination were normally used for inoculations.…”

Section: Methodsmentioning

confidence: 99%

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EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

et al. 2007

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Background: Translation initiation factors of the 4E and 4G protein families mediate resistance to several RNA plant viruses in the natural diversity of crops. Particularly, a single point mutation in melon eukaryotic translation initiation factor 4E (eIF4E) controls resistance to Melon necrotic spot virus (MNSV) in melon. Identification of allelic variants within natural populations by EcoTILLING has become a rapid genotype discovery method.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

et al. 2007

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“…JF737858); CVYV (accession no. AY290865) (Martínez‐García et al ., ); CMV Fny‐strain (accession no. D10538) (Rizzo and Palukaitis, ); and CGMMV (accession no.…”

Section: Methodsmentioning

confidence: 99%

Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

Chandrasekaran

Brumin

Wolf

et al. 2016

Molecular Plant Pathology

739

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Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement in plant traits in addition to those provided by classical breeding. Here, we demonstrate the development of virus resistance in cucumber (Cucumis sativus L.) using Cas9/subgenomic RNA (sgRNA) technology to disrupt the function of the recessive eIF4E (eukaryotic translation initiation factor 4E) gene. Cas9/sgRNA constructs were targeted to the N' and C' termini of the eIF4E gene. Small deletions and single nucleotide polymorphisms (SNPs) were observed in the eIF4E gene targeted sites of transformed T1 generation cucumber plants, but not in putative off-target sites. Non-transgenic heterozygous eif4e mutant plants were selected for the production of non-transgenic homozygous T3 generation plants. Homozygous T3 progeny following Cas9/sgRNA that had been targeted to both eif4e sites exhibited immunity to Cucumber vein yellowing virus (Ipomovirus) infection and resistance to the potyviruses Zucchini yellow mosaic virus and Papaya ring spot mosaic virus-W. In contrast, heterozygous mutant and non-mutant plants were highly susceptible to these viruses. For the first time, virus resistance has been developed in cucumber, non-transgenically, not visibly affecting plant development and without long-term backcrossing, via a new technology that can be expected to be applicable to a wide range of crop plants.

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“…Additionally, mosaic-inducing viruses, such as Cucumber mosaic virus (CMV, genus Cucumovirus) and potyviruses Watermelon mosaic virus (WMV), Papaya ringspot virus (PRSV), and Zucchini yellow mosaic virus (ZYMV), have also become widespread in cucurbit crops in Spain (3,24). Emerging viruses such as Cucumber vein yellowing virus (CVYV, genus Ipomovirus) (26) and Cucurbit aphid-borne yellows virus (CABYV) (18) may become important problems.…”

mentioning

confidence: 99%

Cucurbit aphid-borne yellows virus Is Prevalent in Field-Grown Cucurbit Crops of Southeastern Spain

et al. 2007

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Despite the importance of field-grown cucurbits in Spain, only limited information is available about the impact of disease on their production. During the 2003 and 2004 growing seasons, systematic surveys were carried out in open field melon (Cucumis melo) and squash (Cucurbita pepo) crops of Murcia Province (Spain). The fields were chosen with no previous information regarding their sanitation status, and samples were taken from plants showing viruslike symptoms. Samples were analyzed using molecular hybridization to detect Beet pseudo-yellows virus (BPYV), Cucurbit aphid-borne yellows virus (CABYV), Cucumber mosaic virus (CMV), Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Melon necrotic spot virus (MNSV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). We collected 924 samples from 48 field plots. Out of these, almost 90% were infected by at least one of the viruses considered, usually CABYV, which was present in 83 and 66% of the melon and squash samples, respectively. In the case of melon, CYSDV, BPYV, and WMV followed CABYV in relative importance, with frequencies of around 20 to 30%, while in squash, CVYV and BPYY showed frequencies between 28 and 21%. The number of multiple infections was very high, 66 and 56% of the infected samples of melon and squash, respectively, being afflicted. CABYV was present in all multiple infections. The high incidence of CABYV in single and multiple infections suggests that this virus may well become an important threat for cucurbit crops in the region. Restriction fragment length polymorphism (RFLP) analysis revealed that CABYV isolates can be grouped into two genetic types, both of which seemed to be present during the 2003 epidemic episode, but only one of the types was found in 2004.

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Development and use of detection methods specific for Cucumber vein yellowing virus (CVYV)

Cited by 16 publications

References 20 publications

EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

Cucurbit aphid-borne yellows virus Is Prevalent in Field-Grown Cucurbit Crops of Southeastern Spain

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