Development and Validation of a Quantitative HPLC/MS/MS Method for the Determination of Vancomycin in Blood Plasma

Петров, В. И.; Аникеев, Иван С.; Zayachnikova, T.E.; Стрыгин, А. В.; Доценко, А М; Стрыгина, А О

doi:10.19163/1994-9480-2022-19-4-128-134

Journal of VolgSMU

2022

DOI: 10.19163/1994-9480-2022-19-4-128-134

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Development and Validation of a Quantitative HPLC/MS/MS Method for the Determination of Vancomycin in Blood Plasma

В. И. Петров

Иван С. Аникеев

T.E. Zayachnikova

et al.

Abstract: Within the framework of this study, a quantitative HPLC-MS/MS (high-performance liquid chromatography with mass spectrometric detection) method for the determination of vancomycin was developed. The method for the quantitative determination of vancomycin in human plasma was validated using the chromato-mass spectrometric method. The method was validated in accordance with the requirements of the EAEU for bioanalytical methods. The validated analytical range of the technique was 1-100 pg/mL in plasma.

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2024

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Optimization of therapeutic drug monitoring of vancomycin in newborns using “Dried Blood Spot” method

Petrov,

Anikeev,

Zayachnikova

et al. 2024

Farm. farmakol. (Pâtigorsk)

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Therapeutic drug monitoring (TDM) is used to increase the individualization of pharmacotherapy, especially in patient groups with a high interindividual variability in pharmacokinetic (PK) parameters. One of these groups of patients is newborn children, for whom drug therapy, especially drugs with a narrow therapeutic range, causes a few difficulties or cannot be used in principle.The aim of the work was to develop and validate quantitative HPLC-MS/MS methods for the determination of vancomycin in “dried blood spot” samples using new protocols and comparison of the results obtained with the results in plasma samples using standard sample preparation methods.Materials and methods. To prepare stock and standard solutions of vancomycin and norvancomycin as an internal standard, dry portions of the corresponding certified standards of vancomycin (Servier, France) and norvancomycin (Augsburg, Germany, purity grade >95.0%) were used. A chromatographic separation of the components was carried out on a Poroshell 120 C18 column (4.6×50 mm, 2.7 µm). When developing conditions for a mass spectrometric detection of the desired substances using the multiple reaction monitoring (MRM) method, precursor ions and their corresponding product ions were determined.Results. A quantitative HPLC-MS/MS method for the determination of vancomycin in «dried blood spot» samples was developed and validated. A comparison was made between vancomycin concentrations in «dried blood spot» samples and plasma samples. Moreover, more than 95% of the calculated average concentrations are within the limits of d-2s and d+2s, which correspond to the values of –10.2 and 12.2. That confirms the suitability of the developed method for the analysis of patient samples.Conclusion. The results obtained make it possible for us to recommend the “dried blood spot” method for therapeutic monitoring of vancomycin, additional studies of PK in this group of patients with subsequent use of this drug in newborns and pediatric patients.

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Optimization of therapeutic drug monitoring of vancomycin in newborns using “Dried Blood Spot” method

Petrov,

Anikeev,

Zayachnikova

et al. 2024

Farm. farmakol. (Pâtigorsk)

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show abstract

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Development and Validation of a Quantitative HPLC/MS/MS Method for the Determination of Vancomycin in Blood Plasma

Cited by 1 publication

References 7 publications

Optimization of therapeutic drug monitoring of vancomycin in newborns using “Dried Blood Spot” method

Optimization of therapeutic drug monitoring of vancomycin in newborns using “Dried Blood Spot” method

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