Development and Validation of a Method for Determination of Encapsulation Efficiency of CPT-11/DSPE-mPEG2000 Nanoparticles

Li, Chan; Li, Xianlei

doi:10.4172/2161-0444.1000368

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…To calculate it, we examined centrifuge method for separation of entrapped BBR from the micellar system. Also, we con rmed that centrifuge method compared to other methods (gel chromatography and dialysis method) is the most appropriate for separation of free drug form micellar system [33]. Drug release studies have shown that BBR was released in a declining trend from nano micelle formulations in SGF and SIF, and they are stable for at least 4 hours.…”

Section: Discussionmentioning

confidence: 69%

Anti-inflammatory Efficacy of Berberine Nanomicelle for Improvement of Cerebral Ischemia: Formulation, Characterization and Evaluation in Bilateral Common Carotid Artery Occlusion Rat Model

Azadi

Mousavi

Kazemi

et al. 2020

Preprint

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Backgraound: Berberine (BBR) is a plant alkaloid which possesses anti-inflammatory and anti-oxidant effects with low oral bioavailability. In this study, micelle formulation of BBR was investigated in order to improve therapeutic efficacy and examined its effect on secretion of inflammatory cytokines in cerebral ischemia in animal model. Material and Methods: Nano formulation was prepared by thin film hydration method, and characterized by particle size, zeta potential, morphology, encapsulation efficacy and drug release in Simulated Gastric Fluid (SGF) and Simulated Intestine Fluid (SIF). Then, rats were pretreated with drug (100 mg/kg) and nano drug (25, 50, 75, 100 mg/kg) for 14 days. Stroke induction was accomplished by Bilateral Common Carotid Artery Occlusion (BCCAO), TNF-α, IL-1ß, and MDA levels were measured in the brain and the anti-inflammatory effect of BBR formulations were examined. Result and discussion: Micelles were successfully formed with appropriate characteristics and smaller sizes than 20 nm. The PDI, zeta potential, encapsulation efficacy of micelles were 0.227, -22 mV, 81%, respectively. Also, the stability of nano micelles was higher in SGF as compared to SIF. Conclusion: Our clinical data show that treated groups in different doses (nano BBR 100, 75, 50 mg/kg, and BBR 100 mg/kg) successfully showed decreased levels of the inflammatory factors in cerebral ischemia compared with stroke group and treated group with nano BBR in dose of 25 mg/kg. Nano BBR formulation with lower dose can be a better candidate than conventional BBR formulation to reduce oxidative and inflammatory factors in cerebral ischemia.

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Section: Discussionmentioning

confidence: 69%

Anti-inflammatory Efficacy of Berberine Nanomicelle for Improvement of Cerebral Ischemia: Formulation, Characterization and Evaluation in Bilateral Common Carotid Artery Occlusion Rat Model

Azadi

Mousavi

Kazemi

et al. 2020

Preprint

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“…13,14 Determination of polydispersity indices and particle size The particle size mean diameter (Z. Ave (nm) and polydispersity indices of nanoparticulate lipospheres formulations (PDI) were determined with a Zetasizer nano-ZS (Malvern Instrument, Worceshtire, UK) fortified with a 10mw He-NE laser using wavelength of 633 nm and a backscattering angle of 173 0 at 25 o C. Before photon correlation spectroscopic (PCS) analysis, deionized water was used to dilute all samples to get a suitable scattering intensity. 15…”

Section: Preparation Of Nanoparticulate Lipospheresmentioning

confidence: 99%

Novel Capra hircus-Based Miconazole Nitrate-Loaded Mucoadhesive Nanogels for Enhanced Treatment of Oral Candidiasis

2021

TJNPR

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Fungal infection has been on the increase due to an increase in the use of steroids, HIV infection coupled with resistant to current treatment. The need for developing novel drug delivery system for its management and treatment is necessary. The delivery of a required amount of drug to the target site in the body to achieve the desired therapeutic effect and maintain optimum drug plasma concentrations is the goal of every drug delivery system. 1,2 This means that for drug delivery system to be effective, the delivery rate of the drug is dictated by the body's need. In administrating a drug, different factors must be considered, and these factors include the properties of the drug, the disease involved and the time for desired therapeutic outcome. 3 Thus, there is need to develop new, convenient, and non-parenteral dosage forms with administrative routes that allow a drug to be rapidly dissolved and absorbed into the systemic circulation to achieve optimum plasma concentration. The oral mucosal lining has rich blood supply and drug through this route has rapid onset of action, enhanced

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“…The encapsulation efficiency is a parameter regarded key step in characterizing the quality of the nanostructures (Li et al 2016) and it corresponds to the percentage of the concentration of encapsulated compound by the total concentration of the compound added in the formulation (Couvreur et al 2002). As the loading capacity, the encapsulation efficiency can be determined by ultracentrifugation, size exclusion chromatography or tangential filtration.…”

Section: Loading Capacity Encapsulation Efficiency and Release Profilementioning

confidence: 99%

“…As the loading capacity, the encapsulation efficiency can be determined by ultracentrifugation, size exclusion chromatography or tangential filtration. Another method frequently utilized to determine encapsulation efficiency is the dialysis method (Li et al 2016). The release kinetics provides critical information concerning the dosage form used to assess product safety and efficacy.…”

Section: Loading Capacity Encapsulation Efficiency and Release Profilementioning

confidence: 99%

Nanoencapsulation of carotenoids: a focus on different delivery systems and evaluation parameters

et al. 2018

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Different types of nanoparticles have been synthesized to protect carotenoids against exposition of external factors such as light, heat and oxygen; and processing conditions; to increase stability and to improve the bioavailability of nanoencapsulated carotenoid. The type of nanostructure synthesized (nanoemulsions, liposomes, solid lipid nanoparticles, nanostructured lipid carrier, and polymeric nanoparticles) influences on the synthesis and nanoparticles stability, which reflect in physic-chemical characteristics such as polydispersity index, zeta potential, and encapsulation efficiency. Different nanostructures can be used to improve stability of carotenoids; however, currently, polymeric nanocapsules are the nanostructure most utilized due to its stability during storage, high efficiency to encapsulate and to control the release of the carotenoid encapsulated. Due to these considerations, they have been focus of researchers for future studies regarding to application of carotenoids nanoencapsulated by food industries. The focus of this review is the presentation of different carotenoids delivery systems and the use of techniques to evaluate parameters that might limit the application of this innovative and potential technology in cosmetic, pharmaceutical and food industry.

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Development and Validation of a Method for Determination of Encapsulation Efficiency of CPT-11/DSPE-mPEG2000 Nanoparticles

Cited by 17 publications

References 13 publications

Anti-inflammatory Efficacy of Berberine Nanomicelle for Improvement of Cerebral Ischemia: Formulation, Characterization and Evaluation in Bilateral Common Carotid Artery Occlusion Rat Model

Anti-inflammatory Efficacy of Berberine Nanomicelle for Improvement of Cerebral Ischemia: Formulation, Characterization and Evaluation in Bilateral Common Carotid Artery Occlusion Rat Model

Novel Capra hircus-Based Miconazole Nitrate-Loaded Mucoadhesive Nanogels for Enhanced Treatment of Oral Candidiasis

Nanoencapsulation of carotenoids: a focus on different delivery systems and evaluation parameters

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