Development and Validation of a Novel Bioanalytical Method for Estimating Epigallocatechin 3 Gallate in Wistar Rat Plasma by RP-HPLC Employing Gradient Elution Techniques

Abstract

The goal of this study was to provide a new, easy, accurate, cost-effective, exact, sensitive, specific, robust, and rugged method for quantifying Epigallocatechin 3 gallate in wistar rat plasma using reverse phase high-performance chromatography (RP-HPLC). The stationary phase was a Zorbax SB C18 5µ (4.6*150) mm column, while the mobile phase was water with 0.1 percent formic acid (A) and acetonitrile (ACN) with 0.08 percent formic acid (B). The experiment was conducted at 30°C with a flow rate of 1.0 ml/min with PDA detectors at 274 nm. With an r 2 of 0.9999, the method was shown to be linear in the concentration range of 0.2-25 µg/ml. At the same retention time (Rt) of epigallocatechin 3 gallate, no interference of co-eluting peaks of endogenous chemicals from the biological matrix was found. The intraday and interday precision RSD (%) was found to be within acceptable limits (less than 2%). The overall mean recovery percentage was determined to be 96.92 %. The LOD and LOQ were determined to be 0.0682 ± 0.0011 µg/ml and 0.205 ± 0.004 µg/ml, respectively. In short-term and long-term stability tests, auto sampler, bench-top, and freeze-thaw stability tests were found to be stable. The developed approach reported was determined to be well within acceptable limits. As a result, in the future, this method can be successfully employed in clinical laboratories to estimate epigallocatechin 3 gallate alone or in conjunction with other analytes or markers in pharmacokinetic, bioequivalence, and therapeutic drug monitoring.

Extraction, isolation and chromatographic estimation of Aloe-Emodin and Physcion from Cassia Fistula root

“…The extracts were scanned at 434 and 286 nm in absorbance mode on a CAMAG HPTLC Densitometer (Scanner 3), as well as at 350 and 600 nm with a deuterium and tungsten lamp and a slit size of 6.0 x 0.45 macro. Colour and Rf values of the resolved bands were recorded (Mane et al, 2023).…”

Fabrication of microspheres and characterization of antimicrobial and anti-inflammatory activity isolated fraction from total alcoholic extract of Cassia Fistula (Linn.) in carrageenan-Type-IV induced inflammatory rats

“…Toxicological studies help decide whether new medication should be received for clinical utilization. Enhancing the pharmacological action of Aloe-emodin and Physcion with the features of micro formulation with long-lasting activity may be created (Mane et al, 2023). To do this, a fraction of aloe-emodin and physcion-loaded microspheres were made experimentally and subsequently characterised.…”